FastQCFastQC Report
Thu 26 May 2016
SRR1544792_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544792_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1277819
Sequences flagged as poor quality0
Sequence length52
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGCT64230.5026533491832568TruSeq Adapter, Index 13 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGCTT57680.4513941332849175TruSeq Adapter, Index 16 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGCT46790.36617079570737326TruSeq Adapter, Index 13 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24660.19298507848138116No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC22380.17514217584806613No Hit
TCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGCT19200.1502560221752846TruSeq Adapter, Index 13 (95% over 22bp)
ACCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGC18180.14227367099722263TruSeq Adapter, Index 16 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGC15770.12341340988042906TruSeq Adapter, Index 16 (95% over 21bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA15100.11817010077327071No Hit
GCCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGC14680.11488325028818636TruSeq Adapter, Index 13 (95% over 21bp)
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC14310.11198769152751681No Hit
AGCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGC14200.11112684973380424TruSeq Adapter, Index 16 (95% over 21bp)
ACTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGCT13260.10377056531480594TruSeq Adapter, Index 16 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACCC206.31172E-446.011
CATATAA206.31172E-446.014
CGTATAT301.8615683E-646.029
TAAGTCG206.31172E-446.021
TCGATCA600.046.016
GTTACCC206.31172E-446.024
AATGCGC206.31172E-446.08
CTAACGG600.046.01
CGTAGTC206.31172E-446.031
CGTAGCA253.417372E-546.023
TGCGAAT206.31172E-446.021
CCATATA206.31172E-446.013
CGAATAT1700.046.014
CTCGCTA206.31172E-446.025
TCGTATA301.8615683E-646.028
TCCAATC206.31172E-446.032
GCGATAC405.6115823E-946.08
CATCGAT206.31172E-446.036
TGCTACA301.8615683E-646.018
ACCGTAA206.31172E-446.029
Produced by FastQC (version 0.11.5)