##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1544792_1.fastq.gz File type Conventional base calls Encoding Illumina 1.5 Total Sequences 1277819 Sequences flagged as poor quality 0 Sequence length 52 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.890239540967855 34.0 33.0 34.0 31.0 34.0 2 33.203593779713714 34.0 33.0 34.0 31.0 34.0 3 33.25288479823825 34.0 33.0 34.0 31.0 34.0 4 36.5463880252211 37.0 37.0 37.0 35.0 37.0 5 36.54592395323594 37.0 37.0 37.0 35.0 37.0 6 36.6596881091923 37.0 37.0 37.0 35.0 37.0 7 36.62982707253531 37.0 37.0 37.0 35.0 37.0 8 36.51776268782981 37.0 37.0 37.0 35.0 37.0 9 38.31230088142374 39.0 39.0 39.0 37.0 39.0 10 38.102354089272424 39.0 39.0 39.0 35.0 39.0 11 37.39085973835105 39.0 35.0 39.0 35.0 39.0 12 37.27666672666473 39.0 35.0 39.0 35.0 39.0 13 37.25283706064787 39.0 35.0 39.0 35.0 39.0 14 38.52764436903818 41.0 35.0 41.0 35.0 41.0 15 38.596420932855125 41.0 35.0 41.0 35.0 41.0 16 38.588915957580845 41.0 35.0 41.0 35.0 41.0 17 38.67026002900254 41.0 36.0 41.0 35.0 41.0 18 38.65327170749535 40.0 36.0 41.0 35.0 41.0 19 38.567877766725964 40.0 35.0 41.0 35.0 41.0 20 38.480936658478235 40.0 35.0 41.0 35.0 41.0 21 38.41802399244337 40.0 35.0 41.0 35.0 41.0 22 38.38896275607109 40.0 35.0 41.0 35.0 41.0 23 38.354038404500166 40.0 35.0 41.0 35.0 41.0 24 38.30226190094215 40.0 35.0 41.0 35.0 41.0 25 38.277171492989225 40.0 35.0 41.0 35.0 41.0 26 38.21473620285815 40.0 35.0 41.0 35.0 41.0 27 38.20344430627499 40.0 35.0 41.0 35.0 41.0 28 38.229134955733166 40.0 35.0 41.0 35.0 41.0 29 38.22980562974882 40.0 35.0 41.0 35.0 41.0 30 38.14751854527128 40.0 35.0 41.0 35.0 41.0 31 38.08176431873372 40.0 35.0 41.0 35.0 41.0 32 38.01934624543852 40.0 35.0 41.0 35.0 41.0 33 37.93602615080852 40.0 35.0 41.0 35.0 41.0 34 37.845514114283795 40.0 35.0 41.0 35.0 41.0 35 37.77951337395985 40.0 35.0 41.0 35.0 41.0 36 37.7063613860805 40.0 35.0 41.0 35.0 41.0 37 37.64225919320342 40.0 35.0 41.0 35.0 41.0 38 37.60786934612805 40.0 35.0 41.0 35.0 41.0 39 37.51639238421091 40.0 35.0 41.0 34.0 41.0 40 37.43891192727609 39.0 35.0 41.0 34.0 41.0 41 37.42260132303558 39.0 35.0 41.0 34.0 41.0 42 37.34242251836919 39.0 35.0 41.0 34.0 41.0 43 37.35402510058154 39.0 35.0 41.0 34.0 41.0 44 37.31793704742221 39.0 35.0 41.0 34.0 41.0 45 37.306652194090084 39.0 35.0 41.0 34.0 41.0 46 37.26616915228213 39.0 35.0 41.0 34.0 41.0 47 37.18850009273614 39.0 35.0 41.0 34.0 41.0 48 37.19686121430343 39.0 35.0 41.0 34.0 41.0 49 37.14023817144682 38.0 35.0 41.0 34.0 41.0 50 37.053977910799574 38.0 35.0 41.0 34.0 41.0 51 36.97999716704791 38.0 35.0 41.0 34.0 41.0 52 36.73545470837419 37.0 35.0 40.0 34.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 1.0 11 0.0 12 1.0 13 2.0 14 5.0 15 11.0 16 16.0 17 47.0 18 69.0 19 140.0 20 312.0 21 484.0 22 880.0 23 1334.0 24 2395.0 25 3789.0 26 5541.0 27 6625.0 28 6897.0 29 7605.0 30 9240.0 31 11470.0 32 14994.0 33 21678.0 34 64465.0 35 270263.0 36 49477.0 37 72895.0 38 123950.0 39 597534.0 40 5699.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 33.11744464591621 21.81412234440089 24.42169039590114 20.64674261378176 2 27.48307858937768 23.886246800211925 26.2080153761996 22.422659234210794 3 27.64358645473263 25.17422264029569 25.642911867799747 21.539279037171934 4 26.622080278975346 26.992868317030815 23.098967850689338 23.286083553304497 5 24.950638548965074 34.974280394954214 20.242929554185686 19.832151501895027 6 86.25564340489538 2.9168450304777127 7.896971323794684 2.9305402408322303 7 87.12564142495924 2.609289735087677 5.703624691759944 4.561444148193132 8 76.51036649165492 5.455154446756544 11.11393710689855 6.920541954689983 9 51.0244408636904 20.75583474654861 14.253896678637584 13.965827711123408 10 42.47589056040018 20.544537215364617 23.92686288120618 13.05270934302902 11 40.59049051547989 17.846580775524544 23.64035908058966 17.922569628405903 12 20.837458200261537 34.71508875670185 26.47542414066468 17.97202890237193 13 14.609893889510172 36.58170679885023 29.093322293689482 19.715077017950115 14 14.045181672834728 24.757888245518338 42.94661450487119 18.25031557677574 15 16.31123030726574 18.323721904275956 45.55887805706442 19.806169731393883 16 16.28344859483229 21.518227542398417 27.639595279143602 34.558728583625694 17 19.845064128800715 23.235998212579403 31.796287267602064 25.12265039101782 18 24.503392107958952 27.141637430653326 27.47212242109407 20.882848040293656 19 28.537922820055112 22.742188056367922 24.876684413050675 23.843204710526294 20 21.329546672885595 29.852428239054202 26.829464892915194 21.98856019514501 21 20.412045837477766 24.17955907683326 26.096418976396503 29.311976109292477 22 18.73426518153197 28.651397420135403 24.187854461390852 28.42648293694177 23 19.354306048039668 22.05938399726409 37.14422778186895 21.442082172827295 24 19.584933390409752 22.373826027003823 32.9498152711769 25.09142531140952 25 16.801205804577958 31.25888721329077 28.6445106857857 23.29539629634557 26 17.493870415137042 28.954100698142692 29.178232597887494 24.373796288832768 27 19.736754579482696 26.187120398115855 35.178534675098746 18.89759034730271 28 16.352081163294645 25.6490942770455 31.810921578095176 26.187902981564683 29 19.19786761661863 26.97972091509048 30.82345778236198 22.998953685928917 30 26.50297107806348 24.105057132504683 23.62658561189026 25.765386177541576 31 26.089688758736568 27.851831910466192 24.260634722132007 21.797844608665233 32 22.775134819563647 26.568160279350987 27.070578853499594 23.586126047585772 33 18.988996094126005 26.23532753856376 26.597428900337217 28.178247466973023 34 17.670264724503234 24.664526040072968 29.380608677754832 28.284600557668966 35 25.717726845507855 25.60346966197873 26.008534855092936 22.67026863742048 36 21.30137366872773 26.929713832710267 25.88997346259525 25.878939035966752 37 20.1069165507791 29.317375935089395 25.738934856971134 24.836772657160363 38 18.996352378545005 28.37287597069695 25.257019969181865 27.373751681576188 39 25.068730391393462 22.5746369399735 26.567847245971453 25.78878542266158 40 16.129749205482153 20.610039450031657 31.90537940036891 31.35483194411728 41 20.43646244108125 25.352182116559547 24.142621138048504 30.068734304310706 42 21.2693660056706 22.80534254068847 27.56313687619295 28.362154577447978 43 22.42524175959193 26.638123239676354 27.36756927233043 23.569065728401284 44 17.88547517293138 29.18637146575532 26.467441789486617 26.460711571826685 45 21.839086756418556 26.629749596773877 25.960327714645032 25.570835932162538 46 22.299089307640596 23.251806398245762 27.479322188823303 26.969782105290342 47 20.405472136507598 20.91172536955547 32.829923486816206 25.852879007120727 48 19.364010082805155 19.924418090512034 29.43601558593197 31.27555624075084 49 19.72681576968256 24.03180732169423 31.367822829367853 24.873554079255356 50 18.658432845340382 25.718196395577152 28.458725375033556 27.164645384048914 51 19.70936415877366 21.25692292883421 29.37638272713115 29.657330185260978 52 21.262322754631132 19.992737625594863 32.63020819067489 26.114731429099113 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 226.0 1 473.5 2 721.0 3 2678.5 4 4636.0 5 3120.0 6 1604.0 7 1751.5 8 1899.0 9 1933.0 10 1967.0 11 1921.0 12 1875.0 13 1835.5 14 1720.0 15 1644.0 16 1625.0 17 1606.0 18 1825.0 19 2044.0 20 1883.5 21 1723.0 22 2186.5 23 2650.0 24 3132.0 25 3614.0 26 4780.5 27 5947.0 28 7378.0 29 8809.0 30 10775.5 31 12742.0 32 12954.5 33 13167.0 34 16027.5 35 18888.0 36 20734.0 37 22580.0 38 24330.5 39 31748.5 40 37416.0 41 50793.5 42 64171.0 43 87007.0 44 109843.0 45 119587.5 46 129332.0 47 139994.0 48 150656.0 49 151065.0 50 151474.0 51 135614.5 52 119755.0 53 102994.5 54 86234.0 55 72650.5 56 59067.0 57 52526.0 58 45985.0 59 46893.5 60 47802.0 61 45144.5 62 42487.0 63 39545.5 64 29449.0 65 22294.0 66 17800.0 67 13306.0 68 11349.0 69 9392.0 70 7782.0 71 6172.0 72 5150.5 73 4129.0 74 3214.5 75 2300.0 76 2062.0 77 1824.0 78 1357.0 79 890.0 80 611.0 81 332.0 82 215.0 83 98.0 84 61.5 85 25.0 86 14.5 87 4.0 88 4.5 89 3.5 90 2.0 91 1.5 92 1.0 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 52 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 52 1277819.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 21.120086750582985 #Duplication Level Percentage of deduplicated Percentage of total 1 66.18320597182826 13.97795051556715 2 13.432022137169191 5.673709455455287 3 5.798374610170131 3.6738652433751295 4 3.1486224266378016 2.6599671518168595 5 1.9042447322992757 2.010890697025069 6 1.1636572550423545 1.4745925304663878 7 0.7943458972778304 1.1743657982334221 8 0.5576194223258845 0.942157645866612 9 0.4239998860865648 0.8059422938746997 >10 4.175035016401612 22.540805764286183 >50 2.089228898398682 30.08797674092305 >100 0.31331004721830535 10.559662267741004 >500 0.010022951747503825 1.4093557528243281 >1k 0.0055683065263910135 2.0529584105309877 >5k 7.424408701854685E-4 0.955799732013873 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGCT 6423 0.5026533491832568 TruSeq Adapter, Index 13 (95% over 22bp) CTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGCTT 5768 0.4513941332849175 TruSeq Adapter, Index 16 (95% over 23bp) GCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGCT 4679 0.36617079570737326 TruSeq Adapter, Index 13 (95% over 22bp) CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2466 0.19298507848138116 No Hit GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC 2238 0.17514217584806613 No Hit TCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGCT 1920 0.1502560221752846 TruSeq Adapter, Index 13 (95% over 22bp) ACCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGC 1818 0.14227367099722263 TruSeq Adapter, Index 16 (95% over 21bp) CGCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGC 1577 0.12341340988042906 TruSeq Adapter, Index 16 (95% over 21bp) GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA 1510 0.11817010077327071 No Hit GCCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGC 1468 0.11488325028818636 TruSeq Adapter, Index 13 (95% over 21bp) GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC 1431 0.11198769152751681 No Hit AGCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGC 1420 0.11112684973380424 TruSeq Adapter, Index 16 (95% over 21bp) ACTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGCT 1326 0.10377056531480594 TruSeq Adapter, Index 16 (95% over 22bp) >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.4601590679118091 0.0 2 0.0 0.0 0.0 1.602730903203036 0.0 3 0.0 0.0 0.0 2.497849851974341 0.0 4 0.0 0.0 0.0 3.051684158711054 0.0 5 0.0 0.0 0.0 5.855289364143122 0.0 6 0.0 0.0 0.0 6.324213366681823 0.0 7 0.0 0.0 0.0 8.15483257018404 0.0 8 0.0 0.0 0.0 10.363517837815841 0.0 9 0.0 0.0 0.0 11.987848044206574 0.0 10 0.0 0.0 0.0 13.096768791198128 0.0 11 0.0 0.0 0.0 14.181116417896432 0.0 12 7.825834488296073E-5 0.0 0.0 15.164119487971302 0.0 13 1.5651668976592145E-4 0.0 0.0 15.553376495419148 0.0 14 1.5651668976592145E-4 0.0 0.0 15.845749671901888 0.0 15 2.347750346488822E-4 0.0 0.0 16.796745078919628 0.0 16 2.347750346488822E-4 0.0 0.0 17.824120630543135 0.0 17 2.347750346488822E-4 0.0 0.0 19.24263138989168 0.0 18 2.347750346488822E-4 0.0 0.0 19.892175652420256 0.0 19 2.347750346488822E-4 0.0 0.0 20.65848136551421 0.0 20 2.347750346488822E-4 0.0 0.0 21.585686235687525 0.0 21 2.347750346488822E-4 0.0 0.0 22.374686868797536 0.0 22 2.347750346488822E-4 0.0 0.0 23.30126567221179 0.0 23 2.347750346488822E-4 0.0 0.0 23.972722271307596 0.0 24 2.347750346488822E-4 0.0 0.0 24.526400061354543 0.0 25 2.347750346488822E-4 0.0 0.0 25.054487372624763 0.0 26 2.347750346488822E-4 0.0 0.0 25.533584959998247 0.0 27 2.347750346488822E-4 0.0 0.0 26.026690791105782 0.0 28 3.130333795318429E-4 0.0 0.0 26.526370323183485 0.0 29 3.130333795318429E-4 0.0 0.0 27.190314121170527 0.0 30 3.130333795318429E-4 0.0 0.0 27.831015190727324 0.0 31 3.130333795318429E-4 0.0 0.0 28.40809222589428 0.0 32 3.130333795318429E-4 0.0 0.0 28.947840030552058 0.0 33 3.130333795318429E-4 0.0 0.0 29.46786673229933 0.0 34 3.130333795318429E-4 0.0 0.0 30.179782895699624 0.0 35 3.130333795318429E-4 0.0 0.0 30.709122340487973 0.0 36 3.130333795318429E-4 0.0 0.0 31.276417082544555 0.0 37 3.130333795318429E-4 0.0 0.0 31.806147819057315 0.0 38 3.130333795318429E-4 0.0 0.0 32.30050578368298 0.0 39 3.130333795318429E-4 0.0 0.0 32.862087666563106 0.0 40 3.130333795318429E-4 0.0 0.0 33.430478025448046 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGAACCC 20 6.31172E-4 46.0 11 CATATAA 20 6.31172E-4 46.0 14 CGTATAT 30 1.8615683E-6 46.0 29 TAAGTCG 20 6.31172E-4 46.0 21 TCGATCA 60 0.0 46.0 16 GTTACCC 20 6.31172E-4 46.0 24 AATGCGC 20 6.31172E-4 46.0 8 CTAACGG 60 0.0 46.0 1 CGTAGTC 20 6.31172E-4 46.0 31 CGTAGCA 25 3.417372E-5 46.0 23 TGCGAAT 20 6.31172E-4 46.0 21 CCATATA 20 6.31172E-4 46.0 13 CGAATAT 170 0.0 46.0 14 CTCGCTA 20 6.31172E-4 46.0 25 TCGTATA 30 1.8615683E-6 46.0 28 TCCAATC 20 6.31172E-4 46.0 32 GCGATAC 40 5.6115823E-9 46.0 8 CATCGAT 20 6.31172E-4 46.0 36 TGCTACA 30 1.8615683E-6 46.0 18 ACCGTAA 20 6.31172E-4 46.0 29 >>END_MODULE