FastQCFastQC Report
Thu 26 May 2016
SRR1544791_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544791_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences889760
Sequences flagged as poor quality0
Sequence length52
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGCT75550.8491053767308039TruSeq Adapter, Index 13 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGCTT62970.7077189354432656TruSeq Adapter, Index 16 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGCT42990.48316399928070486TruSeq Adapter, Index 13 (95% over 22bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA33840.38032727926631904No Hit
TGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGCTTG30840.34661032188455315TruSeq Adapter, Index 13 (95% over 24bp)
TCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGCT16330.18353263801474556TruSeq Adapter, Index 13 (95% over 22bp)
ACCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGC16070.18061050170832582TruSeq Adapter, Index 16 (95% over 21bp)
GCCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGC15450.17364233051609423TruSeq Adapter, Index 13 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGC15020.16880956662470778TruSeq Adapter, Index 16 (95% over 21bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC14070.15813253012048192No Hit
AGCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGC13240.1488041719115267TruSeq Adapter, Index 16 (95% over 21bp)
ACTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGCT11110.12486513217047294TruSeq Adapter, Index 16 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10440.11733501168854524No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC9760.10969250134867829No Hit
CGTTCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCT9180.10317388958820356No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCG8950.1005889228556015No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCGC206.3106813E-446.034
ACCGGTA206.3106813E-446.03
TGAACGG301.8609262E-646.01
CACGACG600.046.025
TAACGGA206.3106813E-446.028
TCGATCA253.41653E-546.016
GCGCACT253.41653E-546.021
CGTTAGT206.3106813E-446.022
AACGGGA1200.046.03
TACGAGT206.3106813E-446.030
AGGCACG253.41653E-546.09
TGTTACG206.3106813E-446.015
CGAATAT1200.046.014
GTATCGC206.3106813E-446.05
GCGATAT501.6370905E-1146.08
TCATCGG206.3106813E-446.01
CGCACTA253.41653E-546.022
ATACGAT206.3106813E-446.010
ACGAGTA206.3106813E-446.031
CCGATTT351.01921614E-745.99999611
Produced by FastQC (version 0.11.5)