FastQCFastQC Report
Thu 26 May 2016
SRR1544790_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544790_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1247908
Sequences flagged as poor quality0
Sequence length52
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCTGCT50840.40740182769883676TruSeq Adapter, Index 14 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCTGCTT41480.3323962984450777TruSeq Adapter, Index 15 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCTGCT28980.23222865788183103TruSeq Adapter, Index 14 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24830.19897300121483313No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC24530.19656897784131522No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA14180.11363017145494701No Hit
AGGCAGAATTTGAAACCTAAAGAACTCCCAGATTTCAGGCTTATCCTGTATT14100.11298909855534223No Hit
GGACAGGCAGAATTTGAAACCTAAAGAACTCCCAGATTTCAGGCTTATCCTG13700.10978373405731834No Hit
CGCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCTGC12800.10257166393676458TruSeq Adapter, Index 15 (95% over 21bp)
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC12720.10193059103715979No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTAA206.3116633E-446.036
TCGTTAC206.3116633E-446.010
CCGCTTA253.4173252E-546.029
TACGGGA2450.046.03
GTTACGC206.3116633E-446.012
TATTCCG206.3116633E-446.039
TTGCGTG206.3116633E-446.013
ATGCGTA253.4173252E-546.01
TACAGCG206.3116633E-446.031
AATACGG501.6370905E-1146.01
CCCATAC301.8615319E-646.021
GCTACGA1850.044.75675610
ACGTAGG1000.043.71
CGAATAT1850.043.5135114
GATCCCA800.043.1258
GGCGATA2250.042.9333347
TTCACGG1450.042.8275871
CACGAGG1850.042.2702671
CGTTTTT20050.042.099751
GGGCGAT12800.042.0468756
Produced by FastQC (version 0.11.5)