##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544790_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1247908
Sequences flagged as poor quality	0
Sequence length	52
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.941365068578776	34.0	33.0	34.0	31.0	34.0
2	33.265940277648674	34.0	33.0	34.0	31.0	34.0
3	33.32175048160602	34.0	34.0	34.0	31.0	34.0
4	36.596032720360796	37.0	37.0	37.0	35.0	37.0
5	36.590462598204354	37.0	37.0	37.0	35.0	37.0
6	36.68022482426589	37.0	37.0	37.0	35.0	37.0
7	36.65150956641035	37.0	37.0	37.0	35.0	37.0
8	36.54923680271302	37.0	37.0	37.0	35.0	37.0
9	38.35713930834645	39.0	39.0	39.0	37.0	39.0
10	38.122526660619215	39.0	39.0	39.0	35.0	39.0
11	37.38311157553281	39.0	35.0	39.0	35.0	39.0
12	37.292582466015126	39.0	35.0	39.0	35.0	39.0
13	37.287445068065914	39.0	35.0	39.0	35.0	39.0
14	38.5370588216439	41.0	35.0	41.0	35.0	41.0
15	38.586939902621026	41.0	35.0	41.0	35.0	41.0
16	38.565095343566995	41.0	35.0	41.0	35.0	41.0
17	38.625778502902456	41.0	36.0	41.0	35.0	41.0
18	38.618245094990975	40.0	36.0	41.0	35.0	41.0
19	38.5305415142783	40.0	35.0	41.0	35.0	41.0
20	38.435332572593495	40.0	35.0	41.0	35.0	41.0
21	38.37327110652388	40.0	35.0	41.0	35.0	41.0
22	38.34254848915145	40.0	35.0	41.0	35.0	41.0
23	38.312545476108816	40.0	35.0	41.0	35.0	41.0
24	38.27391762854313	40.0	35.0	41.0	35.0	41.0
25	38.22527221557999	40.0	35.0	41.0	35.0	41.0
26	38.1524335127269	40.0	35.0	41.0	35.0	41.0
27	38.13880189885793	40.0	35.0	41.0	35.0	41.0
28	38.16199271100113	40.0	35.0	41.0	35.0	41.0
29	38.15496895604484	40.0	35.0	41.0	35.0	41.0
30	38.07324898950884	40.0	35.0	41.0	35.0	41.0
31	37.990050548598134	40.0	35.0	41.0	35.0	41.0
32	37.92317462505249	40.0	35.0	41.0	35.0	41.0
33	37.77150479041724	40.0	35.0	41.0	35.0	41.0
34	37.711083669629495	40.0	35.0	41.0	35.0	41.0
35	37.624442667247905	40.0	35.0	41.0	35.0	41.0
36	37.56831112549963	40.0	35.0	41.0	35.0	41.0
37	37.50127333104684	39.0	35.0	41.0	35.0	41.0
38	37.44466018328274	39.0	35.0	41.0	35.0	41.0
39	37.33568259839668	39.0	35.0	41.0	34.0	41.0
40	37.27612291931777	39.0	35.0	41.0	34.0	41.0
41	37.22223593405924	39.0	35.0	41.0	34.0	41.0
42	37.158160697743746	39.0	35.0	41.0	34.0	41.0
43	37.15930821823404	39.0	35.0	41.0	34.0	41.0
44	37.12758953384384	39.0	35.0	41.0	34.0	41.0
45	37.10546210137286	38.0	35.0	41.0	34.0	41.0
46	37.06866692095892	38.0	35.0	41.0	34.0	41.0
47	36.98447641973607	38.0	35.0	41.0	34.0	41.0
48	36.97359260458303	38.0	35.0	41.0	34.0	41.0
49	36.90740423172221	37.0	35.0	41.0	34.0	41.0
50	36.83393487340413	37.0	35.0	41.0	34.0	41.0
51	36.769386845825174	37.0	35.0	41.0	34.0	41.0
52	36.53458748561593	37.0	35.0	40.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	1.0
13	1.0
14	1.0
15	8.0
16	23.0
17	49.0
18	77.0
19	159.0
20	333.0
21	506.0
22	860.0
23	1378.0
24	2465.0
25	3970.0
26	5591.0
27	6669.0
28	6756.0
29	7237.0
30	8646.0
31	10568.0
32	14047.0
33	19672.0
34	67601.0
35	277918.0
36	48502.0
37	74544.0
38	124489.0
39	560594.0
40	5241.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.94849620324575	21.820919490859904	23.870189148559028	20.36039515733532
2	28.26137824262686	23.857447824679383	25.874102898611117	22.00707103408264
3	27.943726620872695	24.843498078383984	25.37855354721662	21.834221753526702
4	27.13325020754735	26.55235802639297	22.86947435227597	23.44491741378371
5	24.760879808447417	35.79158078960949	20.547187773457658	18.900351628485435
6	87.1605919667155	3.1475076688345616	6.99089997019011	2.7010003942598333
7	87.86865698432898	3.2274815130602574	5.191248072774596	3.7126134298361735
8	78.51460203797075	5.636954006224818	9.764101199767932	6.084342756036502
9	53.75244008372412	20.41472608557682	12.860483304859013	12.972350525840046
10	45.037454684159414	20.622914509723472	21.146029995800973	13.193600810316145
11	41.966555226827616	18.390057600400027	22.41471326411883	17.228673908653523
12	22.892793378999095	33.3982152530475	25.395141308493898	18.31385005945951
13	16.179958779012555	35.986386816976896	27.728326126605484	20.105328277405064
14	15.261701984441162	24.972994804104147	41.92063838039343	17.844664831061262
15	18.259118460655756	17.824551168836162	44.44959083522183	19.466739535286255
16	18.762681223295306	20.561692047811217	26.941970081127774	33.733656647765706
17	21.19507207262074	24.457572192821907	30.28716860537796	24.060187129179393
18	26.775451395455434	26.898938062741806	25.05048449084388	21.275126050958885
19	30.486462143042598	22.68091878567971	23.99503809575706	22.837580975520634
20	23.78684967161041	27.765588488895016	26.59763379992756	21.84992803956702
21	21.469932078326288	24.186238088064187	25.663109780528693	28.680720053080837
22	20.458158774525046	28.22419601444978	24.15218109027268	27.165464120752493
23	22.08183616099905	22.06356558336031	35.002179647858654	20.852418607781985
24	21.068620443173696	21.64454430935614	32.01221564410197	25.274619603368198
25	18.282597755603778	30.06944422184969	27.61629863739955	24.03165938514698
26	18.1445266798514	29.115287344900427	28.56717001573834	24.173015959509836
27	19.545831904274994	27.007279382775014	33.71594700891412	19.730941704035875
28	18.093080579658118	26.097035999448675	30.705388538257626	25.10449488263558
29	21.321203165617977	26.40627353939553	29.57718036906567	22.695342925920823
30	27.079480218093	25.51093510098501	22.932059094099884	24.477525586822104
31	28.481827185978453	27.2544931196851	24.331280831599766	19.932398862736676
32	27.708452866717735	25.681781028729684	25.06298541238617	21.546780692166408
33	18.79104869910282	25.942777832981278	29.300557412886207	25.9656160550297
34	22.67522926369572	24.782836555258882	28.441359459190902	24.100574721854496
35	27.86046727803652	23.862576407876222	26.80157511611433	21.47538119797293
36	25.06306554649862	27.463883555518514	26.574234639091987	20.89881625889088
37	23.45453350727778	27.730730149979006	26.797007471704642	22.01772887103857
38	23.154992194937446	26.68001166752677	26.418453924488023	23.746542213047757
39	27.780733836148176	21.58804976007847	28.350006570997223	22.281209832776135
40	18.736156832074162	21.030717007984563	31.174653900768323	29.05847225917295
41	22.0013013779862	25.724412376553403	24.60165332700808	27.672632918452322
42	23.6118367700183	22.029107915006556	27.546021020780376	26.813034294194765
43	22.891110562637632	27.09983428265545	27.54618128900528	22.46287386570164
44	21.25405077938438	28.60419197569052	25.2551470140427	24.886610230882404
45	22.629953490161135	26.670395574032703	26.877061450042795	23.822589485763373
46	24.449799183914198	23.58483157412245	27.3946476823612	24.57072155960215
47	23.417351279100703	20.356388451712785	32.39525670161583	23.831003567570686
48	22.212534898405973	20.27096548784045	29.69970542700263	27.816794186750947
49	20.516015603714376	25.26580485099863	30.436778993323227	23.781400551963767
50	19.55584866833132	26.52046464963763	28.653714857184987	25.26997182484606
51	21.936312612788765	22.13191998128067	29.2262730906445	26.70549431528606
52	23.85929090926575	19.68895142911176	31.958044984085365	24.493712677537125
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	253.0
1	533.5
2	814.0
3	2909.5
4	5005.0
5	3364.5
6	1724.0
7	1838.0
8	1952.0
9	1975.0
10	1998.0
11	1883.5
12	1769.0
13	1768.0
14	1809.5
15	1852.0
16	1693.5
17	1535.0
18	1677.5
19	1820.0
20	1817.5
21	1815.0
22	2353.0
23	2891.0
24	3165.0
25	3439.0
26	3764.5
27	4090.0
28	6517.0
29	8944.0
30	10040.5
31	11137.0
32	12446.0
33	13755.0
34	16428.5
35	19102.0
36	20678.0
37	22254.0
38	25437.0
39	33990.0
40	39360.0
41	50091.0
42	60822.0
43	80703.0
44	100584.0
45	110709.5
46	120835.0
47	124682.0
48	128529.0
49	127416.0
50	126303.0
51	115105.0
52	103907.0
53	94009.0
54	84111.0
55	73459.5
56	62808.0
57	58142.0
58	53476.0
59	54746.5
60	56017.0
61	54103.5
62	52190.0
63	47776.0
64	34777.0
65	26192.0
66	21601.0
67	17010.0
68	14603.0
69	12196.0
70	10088.5
71	7981.0
72	7014.5
73	6048.0
74	5047.5
75	4047.0
76	3646.0
77	3245.0
78	2114.5
79	984.0
80	864.0
81	744.0
82	575.5
83	407.0
84	292.5
85	178.0
86	97.0
87	16.0
88	9.0
89	2.0
90	2.0
91	2.5
92	3.0
93	7.0
94	11.0
95	6.0
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1247908.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.947510100091936
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.79861462217957	14.411596746704392
2	12.69492928355832	5.318543187745816
3	5.100185151696231	3.205085399324872
4	2.553409740636873	2.139503053266561
5	1.414395171273804	1.4814028567889637
6	0.8728022806379613	1.0969820753428183
7	0.6425212713545395	0.9421454574856188
8	0.4481234092211096	0.750965571259746
9	0.34395083738720006	0.6484422276093106
>10	4.576293681610831	25.48088019459074
>50	2.264860186165507	31.83135420213877
>100	0.27572543375123737	9.14482152076879
>500	0.008820146003168841	1.2705871573612
>1k	0.004985299914834563	1.869290203691922
>5k	3.8348460883342786E-4	0.4084001459204869
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCTGCT	5084	0.40740182769883676	TruSeq Adapter, Index 14 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCTGCTT	4148	0.3323962984450777	TruSeq Adapter, Index 15 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCTGCT	2898	0.23222865788183103	TruSeq Adapter, Index 14 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2483	0.19897300121483313	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	2453	0.19656897784131522	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA	1418	0.11363017145494701	No Hit
AGGCAGAATTTGAAACCTAAAGAACTCCCAGATTTCAGGCTTATCCTGTATT	1410	0.11298909855534223	No Hit
GGACAGGCAGAATTTGAAACCTAAAGAACTCCCAGATTTCAGGCTTATCCTG	1370	0.10978373405731834	No Hit
CGCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCTGC	1280	0.10257166393676458	TruSeq Adapter, Index 15 (95% over 21bp)
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	1272	0.10193059103715979	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.3374447475294653	0.0
2	0.0	0.0	0.0	1.1516874641399846	0.0
3	0.0	0.0	0.0	1.8067838334236177	0.0
4	0.0	0.0	0.0	2.2053709087528888	0.0
5	0.0	0.0	0.0	4.266420280982252	0.0
6	0.0	0.0	0.0	4.603143821499661	0.0
7	0.0	0.0	0.0	5.862771935110601	0.0
8	0.0	0.0	0.0	7.377306660426891	0.0
9	0.0	0.0	0.0	8.48932773890383	0.0
10	0.0	0.0	0.0	9.306615551787472	0.0
11	0.0	0.0	0.0	10.104911580020323	0.0
12	8.013411245059733E-5	0.0	0.0	10.828602749561666	0.0
13	8.013411245059733E-5	0.0	0.0	11.138561496520577	0.0
14	8.013411245059733E-5	0.0	0.0	11.349394346378098	0.0
15	1.6026822490119465E-4	0.0	0.0	12.079255842578139	0.0
16	1.6026822490119465E-4	0.0	0.0	12.871782214714546	0.0
17	3.205364498023893E-4	0.0	0.0	13.91464755414662	0.0
18	3.205364498023893E-4	0.0	0.0	14.426704532705937	0.0
19	3.205364498023893E-4	0.0	0.0	15.036925799017235	0.0
20	3.205364498023893E-4	0.0	0.0	15.757491738173007	0.0
21	3.205364498023893E-4	0.0	0.0	16.390631360645177	0.0
22	3.205364498023893E-4	0.0	0.0	17.12794532930312	0.0
23	3.205364498023893E-4	0.0	0.0	17.66396240748517	0.0
24	3.205364498023893E-4	0.0	0.0	18.11639960638124	0.0
25	3.205364498023893E-4	0.0	0.0	18.53261618644964	0.0
26	3.205364498023893E-4	0.0	0.0	18.92134676594749	0.0
27	4.006705622529866E-4	0.0	0.0	19.33435798151787	0.0
28	4.006705622529866E-4	0.0	0.0	19.74640758773884	0.0
29	4.006705622529866E-4	0.0	0.0	20.282184263583535	0.0
30	4.006705622529866E-4	0.0	0.0	20.820765633363997	0.0
31	4.006705622529866E-4	0.0	0.0	21.297884138894855	0.0
32	4.006705622529866E-4	0.0	0.0	21.778528545373536	0.0
33	4.006705622529866E-4	0.0	0.0	22.24162358122554	0.0
34	4.006705622529866E-4	0.0	0.0	22.824599249303635	0.0
35	4.006705622529866E-4	0.0	0.0	23.26830182994259	0.0
36	4.006705622529866E-4	0.0	0.0	23.76409158367444	0.0
37	4.006705622529866E-4	0.0	0.0	24.214845966209047	0.0
38	4.006705622529866E-4	0.0	0.0	24.67273228475176	0.0
39	4.006705622529866E-4	0.0	0.0	25.195687502604358	0.0
40	4.006705622529866E-4	0.0	0.0	25.740439199043518	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGCGTAA	20	6.3116633E-4	46.0	36
TCGTTAC	20	6.3116633E-4	46.0	10
CCGCTTA	25	3.4173252E-5	46.0	29
TACGGGA	245	0.0	46.0	3
GTTACGC	20	6.3116633E-4	46.0	12
TATTCCG	20	6.3116633E-4	46.0	39
TTGCGTG	20	6.3116633E-4	46.0	13
ATGCGTA	25	3.4173252E-5	46.0	1
TACAGCG	20	6.3116633E-4	46.0	31
AATACGG	50	1.6370905E-11	46.0	1
CCCATAC	30	1.8615319E-6	46.0	21
GCTACGA	185	0.0	44.756756	10
ACGTAGG	100	0.0	43.7	1
CGAATAT	185	0.0	43.51351	14
GATCCCA	80	0.0	43.125	8
GGCGATA	225	0.0	42.933334	7
TTCACGG	145	0.0	42.827587	1
CACGAGG	185	0.0	42.270267	1
CGTTTTT	2005	0.0	42.09975	1
GGGCGAT	1280	0.0	42.046875	6
>>END_MODULE