FastQCFastQC Report
Thu 26 May 2016
SRR1544789_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544789_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences937877
Sequences flagged as poor quality0
Sequence length52
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCTGCT63060.6723696177643764TruSeq Adapter, Index 14 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCTGCTT51220.5461270507753149TruSeq Adapter, Index 15 (95% over 23bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA45230.48225940075297724No Hit
GCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCTGCT38110.4063432624960416TruSeq Adapter, Index 14 (95% over 22bp)
TGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCTGCTTG26180.27914108139979976TruSeq Adapter, Index 14 (95% over 24bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC16230.17305041066152596No Hit
TCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCTGCT13830.14746070113671622TruSeq Adapter, Index 14 (95% over 22bp)
ACCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCTGC13720.14628783945016244TruSeq Adapter, Index 15 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCTGC12670.13509234153305819TruSeq Adapter, Index 15 (95% over 21bp)
GCCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCTGC12150.12954790446934938TruSeq Adapter, Index 14 (95% over 21bp)
AGCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCTGC11150.11888552550067867TruSeq Adapter, Index 15 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10980.11707292107600463No Hit
AGGCAGAATTTGAAACCTAAAGAACTCCCAGATTTCAGGCTTATCCTGTATT10690.11398083117509013No Hit
ACTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCTGCT9760.10406481873422634TruSeq Adapter, Index 15 (95% over 22bp)
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC9420.1004396098848783No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACTCG206.3108583E-446.033
TTTACGC253.416671E-546.041
CGCGGAC253.416671E-546.014
TAAACGG206.3108583E-446.01
CGCGATT206.3108583E-446.042
GTCACGG1050.046.01
GTATCGG301.8610335E-646.01
GCGATAA206.3108583E-446.08
TTCGCGA206.3108583E-446.040
AATACGG301.8610335E-646.01
TAGTCGG301.8610335E-646.01
TCGCGAT206.3108583E-446.041
CGAATAT1300.044.2307714
TACGGGA2200.043.9090923
TAGTAGG1900.043.578951
ACGGGAT2700.043.4444474
CTAACGG800.043.1251
GCTACGA1450.042.82758710
GGGCGAT9350.042.802146
GACTAAT3550.042.7605638
Produced by FastQC (version 0.11.5)