##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544789_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	937877
Sequences flagged as poor quality	0
Sequence length	52
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.92339507206169	34.0	33.0	34.0	31.0	34.0
2	33.27139806179275	34.0	34.0	34.0	31.0	34.0
3	33.36550635104603	34.0	34.0	34.0	31.0	34.0
4	36.58923824765934	37.0	37.0	37.0	35.0	37.0
5	36.595075900144685	37.0	37.0	37.0	35.0	37.0
6	36.51915762941196	37.0	37.0	37.0	35.0	37.0
7	36.38643766719943	37.0	37.0	37.0	35.0	37.0
8	36.43305145557466	37.0	37.0	37.0	35.0	37.0
9	38.293609929660285	39.0	39.0	39.0	37.0	39.0
10	38.14115603645254	39.0	39.0	39.0	35.0	39.0
11	37.50187178062795	39.0	35.0	39.0	35.0	39.0
12	37.462259976521445	39.0	35.0	39.0	35.0	39.0
13	37.46189319068492	39.0	35.0	39.0	35.0	39.0
14	38.70298663897292	41.0	35.0	41.0	35.0	41.0
15	38.747205657031785	41.0	35.0	41.0	35.0	41.0
16	38.7372619224056	41.0	35.0	41.0	35.0	41.0
17	38.80585727126265	41.0	36.0	41.0	35.0	41.0
18	38.8132590947427	41.0	36.0	41.0	35.0	41.0
19	38.74200454857087	41.0	35.0	41.0	35.0	41.0
20	38.66380879369043	41.0	35.0	41.0	35.0	41.0
21	38.6053832218937	41.0	35.0	41.0	35.0	41.0
22	38.58290479455195	41.0	35.0	41.0	35.0	41.0
23	38.568344249832336	41.0	35.0	41.0	35.0	41.0
24	38.500824735013225	40.0	35.0	41.0	35.0	41.0
25	38.44833277711256	40.0	35.0	41.0	35.0	41.0
26	38.37563561106627	40.0	35.0	41.0	35.0	41.0
27	38.372186331469905	40.0	35.0	41.0	35.0	41.0
28	38.421256731959524	40.0	35.0	41.0	35.0	41.0
29	38.45022321690371	40.0	36.0	41.0	35.0	41.0
30	38.384283866647756	40.0	35.0	41.0	35.0	41.0
31	38.33145497757169	40.0	35.0	41.0	35.0	41.0
32	38.292085209467764	40.0	35.0	41.0	35.0	41.0
33	38.15449360630445	40.0	35.0	41.0	35.0	41.0
34	38.129532977138794	40.0	35.0	41.0	35.0	41.0
35	38.010535496658946	40.0	35.0	41.0	35.0	41.0
36	38.00300892334496	40.0	35.0	41.0	35.0	41.0
37	37.9438444486857	40.0	35.0	41.0	35.0	41.0
38	37.86584274910249	40.0	35.0	41.0	35.0	41.0
39	37.835280106026694	40.0	35.0	41.0	35.0	41.0
40	37.80611636707159	40.0	35.0	41.0	35.0	41.0
41	37.75958254653862	40.0	35.0	41.0	35.0	41.0
42	37.72836416715625	40.0	35.0	41.0	35.0	41.0
43	37.657967942491396	39.0	35.0	41.0	35.0	41.0
44	37.53948012372624	39.0	35.0	41.0	35.0	41.0
45	37.527341005270415	39.0	35.0	41.0	35.0	41.0
46	37.485809972949546	39.0	35.0	41.0	35.0	41.0
47	37.413789867967765	39.0	35.0	41.0	35.0	41.0
48	37.37389977577017	39.0	35.0	41.0	35.0	41.0
49	37.323161779209855	39.0	35.0	41.0	35.0	41.0
50	37.26079965709789	39.0	35.0	41.0	35.0	41.0
51	37.19955068735026	38.0	35.0	41.0	34.0	41.0
52	36.804080918926466	37.0	35.0	40.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	0.0
13	5.0
14	10.0
15	10.0
16	24.0
17	38.0
18	75.0
19	130.0
20	170.0
21	338.0
22	528.0
23	817.0
24	1356.0
25	2225.0
26	3219.0
27	3710.0
28	3809.0
29	4230.0
30	5015.0
31	6142.0
32	8485.0
33	12490.0
34	43535.0
35	196139.0
36	34360.0
37	54633.0
38	93885.0
39	459675.0
40	2822.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.235168364295106	21.52862262322245	22.76524533600888	22.47096367647357
2	27.812389044618858	23.840439631209637	25.790588744579512	22.556582579591993
3	28.145588387389818	24.587659149334083	25.171850893027553	22.094901570248553
4	27.13042328578268	25.839529064045713	23.229485316304803	23.800562333866807
5	24.56953310508734	34.816825660507725	20.610805041599274	20.002836192805667
6	84.78670443992122	3.1468945288134798	8.309405177864475	3.756995853400819
7	85.66379173388408	3.26961851074288	6.074144050872342	4.992445704500697
8	74.39866848211439	5.755125672129714	11.84600965798287	8.000196187773023
9	51.3840301020283	19.68765627049176	14.262744475021776	14.665569152458158
10	42.30704026220922	19.831918257937875	24.037053899391925	13.823987580460978
11	39.0963847071631	17.996816213639953	23.475892894270782	19.43090618492617
12	22.02655572105937	31.054498617622567	27.274152154280358	19.644793507037704
13	15.883959197208164	33.913615538071625	29.00167079478439	21.200754469935823
14	15.010497112094656	24.826922933391053	40.423957512552285	19.738622441962008
15	17.599962468426032	17.96227010578146	43.669265799246595	20.768501626545913
16	17.844983937126084	22.174123045985773	27.046723610878615	32.93416940600953
17	20.089308086241587	24.678182746778095	30.312077170034023	24.920431996946295
18	25.142955846022453	27.327464049123712	25.98347118012277	21.54610892473107
19	28.4778281160536	22.58750347860114	24.577210017944783	24.35745838740048
20	22.381719564505794	28.708455373145945	27.08329557074115	21.826529491607108
21	20.617415716559847	24.072346373778224	26.063545646177484	29.24669226348444
22	19.663132798863817	28.156250766358486	24.223219036184915	27.957397398592782
23	20.8792837440304	22.166979252076764	35.25313020790573	21.700606795987106
24	21.454305841810815	21.696235220609953	31.213901183204197	25.63555775437504
25	17.935294286990725	29.207241461300367	27.779548917395353	25.07791533431356
26	17.71980760803389	28.019772315559504	28.543508370500607	25.716911705906
27	20.795477445336648	25.974301534209708	32.59638524028204	20.6338357801716
28	18.35091381918951	25.777260770868676	29.553662153992477	26.31816325594934
29	21.856490776509073	25.328374616287636	29.4026828677961	23.412451739407192
30	26.502409164527975	24.930774504545905	22.810347199046358	25.75646913187977
31	28.149106972449477	26.501982669369223	24.208078458049403	21.140831900131893
32	27.62131921350028	24.226097878506454	25.629160326993837	22.523422580999426
33	18.59636178304831	24.737358950054215	30.26228386025033	26.40399540664714
34	24.21266328100593	23.317663190375708	28.30189886307053	24.167774665547828
35	27.155053381200307	22.813439288947272	28.310428766245465	21.721078563606955
36	26.69017365816626	26.076980243678012	26.14031477475191	21.092531323403815
37	23.977451200957056	26.462851738554207	28.23995044126255	21.319746619226187
38	23.65256851378166	25.47103724688845	26.2462988217005	24.63009541762939
39	29.149238119710795	20.004222302071597	28.853676974699237	21.992862603518372
40	19.23354555021607	19.71164662317127	31.948645717935293	29.106162108677363
41	22.19363519949844	24.92533669127188	24.884393156032186	27.996634953197486
42	23.27618653618758	20.510045560345333	28.474629402362993	27.739138501104087
43	24.04174534613814	25.48180624964681	27.56875368518473	22.90769471903032
44	21.554532204116317	26.458693410756425	26.49857070809925	25.48820367702801
45	22.18297282052977	24.79589541059222	27.584853877427424	25.436277891450587
46	25.095295012032494	21.483734007764344	27.930954698750476	25.49001628145269
47	22.327128184186197	19.306902717520526	33.796009498047184	24.56995960024609
48	22.226581950511633	18.74787418819312	29.481371224584883	29.544172636710357
49	20.960744319351047	22.865684945893758	31.579940653198662	24.593630081556537
50	19.905488672821704	23.82092747769697	30.21131768878009	26.062266160701242
51	21.944242155421236	20.18889470580897	29.546838231452526	28.320024907317272
52	23.251449816980266	18.498374520326227	34.081548006828186	24.168627655865322
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	123.0
1	292.0
2	461.0
3	1474.5
4	2488.0
5	1747.0
6	1006.0
7	1027.0
8	1048.0
9	1076.5
10	1105.0
11	1072.0
12	1039.0
13	1102.5
14	1152.5
15	1139.0
16	1030.5
17	922.0
18	1044.0
19	1166.0
20	1135.0
21	1104.0
22	1534.5
23	1965.0
24	2099.0
25	2233.0
26	2478.0
27	2723.0
28	4196.0
29	5669.0
30	6329.5
31	6990.0
32	7751.5
33	8513.0
34	10398.0
35	12283.0
36	13422.5
37	14562.0
38	16968.5
39	22615.0
40	25855.0
41	33602.0
42	41349.0
43	54963.5
44	68578.0
45	78781.0
46	88984.0
47	98478.0
48	107972.0
49	109872.5
50	111773.0
51	99818.0
52	87863.0
53	77631.0
54	67399.0
55	57800.5
56	48202.0
57	43468.5
58	38735.0
59	38630.5
60	38526.0
61	37272.0
62	36018.0
63	34665.0
64	25755.5
65	18199.0
66	15145.0
67	12091.0
68	10388.0
69	8685.0
70	7265.5
71	5846.0
72	5173.5
73	4501.0
74	3724.5
75	2948.0
76	2638.5
77	2329.0
78	1520.0
79	711.0
80	619.0
81	527.0
82	400.5
83	274.0
84	187.0
85	100.0
86	52.0
87	4.0
88	6.0
89	5.0
90	2.0
91	2.5
92	3.0
93	3.0
94	3.0
95	1.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	937877.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.136333221412325
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.75478063289205	16.594882959205894
2	12.7608828898729	6.160018432587828
3	4.866810222040206	3.524008597336144
4	2.3921038970504553	2.309464670377952
5	1.358966844917996	1.6400238302896057
6	0.8505141611617254	1.2316975921997633
7	0.6128020065582127	1.0353555400327386
8	0.4397887252110432	0.8491909774971101
9	0.35660529473833774	0.7746429800092027
>10	6.751144736454022	44.19035594542648
>50	0.6897143163866365	10.578075058530365
>100	0.1516927948369466	6.240924036346099
>500	0.008427377490941476	1.389691143745861
>1k	0.0048790080210713805	2.258234726613912
>5k	8.870923674675239E-4	1.2234335098010707
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCTGCT	6306	0.6723696177643764	TruSeq Adapter, Index 14 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCTGCTT	5122	0.5461270507753149	TruSeq Adapter, Index 15 (95% over 23bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA	4523	0.48225940075297724	No Hit
GCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCTGCT	3811	0.4063432624960416	TruSeq Adapter, Index 14 (95% over 22bp)
TGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCTGCTTG	2618	0.27914108139979976	TruSeq Adapter, Index 14 (95% over 24bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	1623	0.17305041066152596	No Hit
TCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCTGCT	1383	0.14746070113671622	TruSeq Adapter, Index 14 (95% over 22bp)
ACCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCTGC	1372	0.14628783945016244	TruSeq Adapter, Index 15 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCTGC	1267	0.13509234153305819	TruSeq Adapter, Index 15 (95% over 21bp)
GCCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCTGC	1215	0.12954790446934938	TruSeq Adapter, Index 14 (95% over 21bp)
AGCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCTGC	1115	0.11888552550067867	TruSeq Adapter, Index 15 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1098	0.11707292107600463	No Hit
AGGCAGAATTTGAAACCTAAAGAACTCCCAGATTTCAGGCTTATCCTGTATT	1069	0.11398083117509013	No Hit
ACTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCTGCT	976	0.10406481873422634	TruSeq Adapter, Index 15 (95% over 22bp)
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	942	0.1004396098848783	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.5564695583749255	0.0
2	0.0	0.0	0.0	1.9077128450745673	0.0
3	0.0	0.0	0.0	2.875003865112376	0.0
4	0.0	0.0	0.0	3.5021649960495886	0.0
5	1.0662378968670731E-4	0.0	0.0	6.582419656308876	0.0
6	1.0662378968670731E-4	0.0	0.0	7.0676645231730815	0.0
7	1.0662378968670731E-4	0.0	0.0	8.941257755547902	0.0
8	1.0662378968670731E-4	0.0	0.0	11.140799912994988	0.0
9	1.0662378968670731E-4	0.0	0.0	12.734292449862828	0.0
10	1.0662378968670731E-4	0.0	0.0	13.912378702111258	0.0
11	1.0662378968670731E-4	0.0	0.0	15.026064185388915	0.0
12	1.0662378968670731E-4	0.0	0.0	16.03237951245206	0.0
13	1.0662378968670731E-4	0.0	0.0	16.469750297746934	0.0
14	1.0662378968670731E-4	0.0	0.0	16.771175751191254	0.0
15	1.0662378968670731E-4	0.0	0.0	17.73377532448285	0.0
16	1.0662378968670731E-4	0.0	0.0	18.718659269818964	0.0
17	1.0662378968670731E-4	0.0	0.0	19.978312721177723	0.0
18	1.0662378968670731E-4	0.0	0.0	20.62285352983387	0.0
19	1.0662378968670731E-4	0.0	0.0	21.35205362750126	0.0
20	1.0662378968670731E-4	0.0	0.0	22.23383236821033	0.0
21	1.0662378968670731E-4	0.0	0.0	23.009733685760498	0.0
22	3.19871369060122E-4	0.0	0.0	23.88436863256056	0.0
23	3.19871369060122E-4	0.0	0.0	24.496495809151945	0.0
24	3.19871369060122E-4	0.0	0.0	25.029934628954543	0.0
25	4.2649515874682926E-4	0.0	0.0	25.539169848498258	0.0
26	4.2649515874682926E-4	0.0	0.0	26.004262819111673	0.0
27	4.2649515874682926E-4	0.0	0.0	26.468822670776657	0.0
28	4.2649515874682926E-4	0.0	0.0	26.937540850239422	0.0
29	4.2649515874682926E-4	0.0	0.0	27.535913557961226	0.0
30	4.2649515874682926E-4	0.0	0.0	28.137591603163315	0.0
31	4.2649515874682926E-4	0.0	0.0	28.65898193473131	0.0
32	4.2649515874682926E-4	0.0	0.0	29.178666285664324	0.0
33	4.2649515874682926E-4	0.0	0.0	29.666363499691325	0.0
34	4.2649515874682926E-4	0.0	0.0	30.285847717771095	0.0
35	4.2649515874682926E-4	0.0	0.0	30.7574447395554	0.0
36	4.2649515874682926E-4	0.0	0.0	31.282140408603688	0.0
37	4.2649515874682926E-4	0.0	0.0	31.763440195249483	0.0
38	4.2649515874682926E-4	0.0	0.0	32.2533765088599	0.0
39	4.2649515874682926E-4	0.0	0.0	32.80888645312765	0.0
40	4.2649515874682926E-4	0.0	0.0	33.367488487296306	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGACTCG	20	6.3108583E-4	46.0	33
TTTACGC	25	3.416671E-5	46.0	41
CGCGGAC	25	3.416671E-5	46.0	14
TAAACGG	20	6.3108583E-4	46.0	1
CGCGATT	20	6.3108583E-4	46.0	42
GTCACGG	105	0.0	46.0	1
GTATCGG	30	1.8610335E-6	46.0	1
GCGATAA	20	6.3108583E-4	46.0	8
TTCGCGA	20	6.3108583E-4	46.0	40
AATACGG	30	1.8610335E-6	46.0	1
TAGTCGG	30	1.8610335E-6	46.0	1
TCGCGAT	20	6.3108583E-4	46.0	41
CGAATAT	130	0.0	44.23077	14
TACGGGA	220	0.0	43.909092	3
TAGTAGG	190	0.0	43.57895	1
ACGGGAT	270	0.0	43.444447	4
CTAACGG	80	0.0	43.125	1
GCTACGA	145	0.0	42.827587	10
GGGCGAT	935	0.0	42.80214	6
GACTAAT	355	0.0	42.760563	8
>>END_MODULE