FastQCFastQC Report
Thu 26 May 2016
SRR1544788_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544788_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1691402
Sequences flagged as poor quality0
Sequence length52
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTGCT54840.324228066420638TruSeq Adapter, Index 21 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTGCTT42690.2523941676786477TruSeq Adapter, Index 15 (96% over 25bp)
GCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTGCT34870.20616033326199212TruSeq Adapter, Index 21 (95% over 22bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC29910.17683554826114667No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29120.17216486677915718No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTAT206.3123036E-446.046
CGTAACG253.4178443E-546.023
CGGTCTA453.110472E-1046.030
GTAACGA206.3123036E-446.024
ATTACGG1350.044.29631
ATAGCGG2450.044.1224481
GTTACGG1800.043.4444471
CACGACC5650.042.74336226
GCGATAA650.042.4615368
GGGCGAT18750.041.9526
TACGGGT554.7293724E-1141.818183
CTACCGG1050.041.6190451
CTAGCGG5350.041.2710271
ATTGCGG4300.041.1860471
GCGAGAC6150.041.1382120
ACGGGAT7000.041.071434
ACACGAC5900.040.932225
CGTATCA451.589251E-840.8888938
AACACGT5800.040.8448340
TTACGGG5250.040.7428552
Produced by FastQC (version 0.11.5)