##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544788_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1691402
Sequences flagged as poor quality	0
Sequence length	52
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.93287402994675	34.0	33.0	34.0	31.0	34.0
2	33.26857187114595	34.0	33.0	34.0	31.0	34.0
3	33.33529462540543	34.0	34.0	34.0	31.0	34.0
4	36.60428035440422	37.0	37.0	37.0	35.0	37.0
5	36.606174049693685	37.0	37.0	37.0	35.0	37.0
6	36.68494302359817	37.0	37.0	37.0	35.0	37.0
7	36.65788381472885	37.0	37.0	37.0	35.0	37.0
8	36.555750200129836	37.0	37.0	37.0	35.0	37.0
9	38.36518521321366	39.0	39.0	39.0	37.0	39.0
10	38.15827697968904	39.0	39.0	39.0	35.0	39.0
11	37.48744946500004	39.0	35.0	39.0	35.0	39.0
12	37.40773926009311	39.0	35.0	39.0	35.0	39.0
13	37.39367991760681	39.0	35.0	39.0	35.0	39.0
14	38.68076483296106	41.0	35.0	41.0	35.0	41.0
15	38.71914009797789	41.0	35.0	41.0	35.0	41.0
16	38.66495605420828	41.0	35.0	41.0	35.0	41.0
17	38.73897689608975	41.0	36.0	41.0	35.0	41.0
18	38.73498257658439	41.0	36.0	41.0	35.0	41.0
19	38.67626501564974	41.0	35.0	41.0	35.0	41.0
20	38.59879082559912	41.0	35.0	41.0	35.0	41.0
21	38.538590471100306	40.0	35.0	41.0	35.0	41.0
22	38.5168641162775	40.0	35.0	41.0	35.0	41.0
23	38.47186889929183	40.0	35.0	41.0	35.0	41.0
24	38.397260379259336	40.0	35.0	41.0	35.0	41.0
25	38.36501198414097	40.0	35.0	41.0	35.0	41.0
26	38.29162020619581	40.0	35.0	41.0	35.0	41.0
27	38.2684483050156	40.0	35.0	41.0	35.0	41.0
28	38.29666631587287	40.0	35.0	41.0	35.0	41.0
29	38.25788074035623	40.0	35.0	41.0	35.0	41.0
30	38.1992116599129	40.0	35.0	41.0	35.0	41.0
31	38.149412144481325	40.0	35.0	41.0	35.0	41.0
32	38.05669675216182	40.0	35.0	41.0	35.0	41.0
33	37.928733677741896	40.0	35.0	41.0	35.0	41.0
34	37.865583699203384	40.0	35.0	41.0	35.0	41.0
35	37.77642098093771	40.0	35.0	41.0	35.0	41.0
36	37.73246159103513	40.0	35.0	41.0	35.0	41.0
37	37.64495253050428	40.0	35.0	41.0	35.0	41.0
38	37.5873417437132	40.0	35.0	41.0	35.0	41.0
39	37.47908480656875	39.0	35.0	41.0	34.0	41.0
40	37.40764407278695	39.0	35.0	41.0	34.0	41.0
41	37.36249868452325	39.0	35.0	41.0	34.0	41.0
42	37.29181826673966	39.0	35.0	41.0	34.0	41.0
43	37.30625776722506	39.0	35.0	41.0	34.0	41.0
44	37.27483531413585	39.0	35.0	41.0	34.0	41.0
45	37.23278380893483	39.0	35.0	41.0	34.0	41.0
46	37.18102970198687	39.0	35.0	41.0	34.0	41.0
47	37.0975031364513	38.0	35.0	41.0	34.0	41.0
48	37.06020744920486	38.0	35.0	41.0	34.0	41.0
49	37.008477582502564	38.0	35.0	41.0	34.0	41.0
50	36.92339313776382	38.0	35.0	41.0	34.0	41.0
51	36.8405600797445	37.0	35.0	41.0	34.0	41.0
52	36.56048769009378	37.0	35.0	40.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	2.0
13	6.0
14	10.0
15	18.0
16	38.0
17	59.0
18	134.0
19	236.0
20	444.0
21	715.0
22	1178.0
23	1931.0
24	3025.0
25	4904.0
26	7137.0
27	8466.0
28	8897.0
29	9802.0
30	11638.0
31	14210.0
32	18680.0
33	27228.0
34	92095.0
35	331932.0
36	69254.0
37	106527.0
38	181035.0
39	785986.0
40	5814.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.82525147776815	21.659841953598256	24.34731660480477	19.167589963828824
2	28.163499865791806	23.94303660513586	25.897687244073257	21.99577628499907
3	28.685079005464104	24.878000617239426	24.273590784449823	22.163329592846644
4	26.41548254051964	26.523854175411877	22.815806059115456	24.244857224953027
5	24.956278873975553	35.827023971829284	20.458116994067645	18.758580160127515
6	88.2110816943577	2.921422583158823	6.369213232572742	2.498282489910737
7	88.94839902045759	2.792535423276075	4.918464090736561	3.340601465529779
8	79.32064642231711	5.187944675482234	9.762433767962909	5.728975134237751
9	52.685760097244774	21.66776437535252	12.727666160971784	12.918809366430924
10	42.13197099211187	22.372978156582526	21.67822906677419	13.816821784531413
11	40.001608133371015	18.492233070553304	23.207315587896904	18.298843208178777
12	23.500859050657382	29.990327550753754	25.937535843046184	20.57127755554268
13	17.399293603767763	32.25998313824863	29.917606813755686	20.423116444227922
14	18.267626501564973	24.573164747351605	38.80904716915317	18.35016158193026
15	21.52616586713271	18.192481740000307	41.0925965559932	19.18875583687379
16	19.85849608786084	20.624783463659142	28.571741076337855	30.94497937214216
17	23.119991580948824	23.655168907214254	28.89064811322205	24.334191398614877
18	25.828691227750706	27.922279860139692	25.137193878214642	21.11183503389496
19	31.289249983150075	23.39964124436414	23.3020299136456	22.00907885884018
20	24.983770859913847	28.818636846828845	25.13370564774075	21.06388664551656
21	22.09468831182652	23.518654938329266	27.032308108894277	27.354348640949933
22	23.813499097198658	27.307227968277203	23.955688830922515	24.92358410360163
23	25.060098072486614	21.380724393136582	32.11442341915169	21.44475411522512
24	21.911644895772856	21.28346779772047	30.234976664329356	26.56991064217732
25	19.86482220075417	29.124832535375976	27.577418023627736	23.43292724024212
26	19.2620086768255	28.207900901145916	27.377761170910286	25.152329251118307
27	20.721330588470394	28.000676361976634	31.65137560438027	19.626617445172702
28	20.982534016159377	25.12359569162151	29.625718782406548	24.26815150981257
29	22.032254898598914	24.82644575328633	30.153210177119337	22.988089170995423
30	27.50842200730518	26.228300545937632	22.942978665036463	23.320298781720727
31	29.192882590892054	25.75957696632734	23.266674628503456	21.78086581427715
32	27.144463587012428	26.10166004297027	24.40342390513905	22.35045246487825
33	19.401597018331536	28.97661230151082	27.331231723741606	24.29055895641604
34	21.7394800289937	25.51971677933454	27.56713069985728	25.17367249181448
35	24.871497136694884	26.331705886595852	25.378059148564326	23.418737828144938
36	24.47910076965736	29.919439612818245	24.758277452669443	20.843182164854955
37	22.952852130954085	29.29953967182255	25.223808414557862	22.523799782665506
38	22.642281373676983	26.73840991083137	24.865644004204796	25.75366471128685
39	25.380305805479715	24.120167766149027	25.903835989315372	24.595690439055883
40	18.486202570412004	24.176334189033714	30.410215903729572	26.927247336824717
41	21.554899426629508	26.31456034697842	23.903897476767792	28.226642749624276
42	22.073167703479125	24.219789263581337	25.984065290214865	27.722977742724673
43	21.954981725219668	27.134530998544403	26.486193110803935	24.424294165431988
44	21.296356513708744	28.315858678185318	24.55909358035523	25.828691227750706
45	21.744268955576498	27.7256973800433	25.721561166416972	24.808472497963226
46	23.030953020038996	24.523856540313894	26.922340165141108	25.522850274506006
47	22.530657998512478	21.605626574876936	31.18850515726007	24.675210269350515
48	21.626851570472304	22.48152715912598	28.1189805853369	27.772640685064815
49	20.07949618127447	25.856774439193046	28.519122006477467	25.544607373055015
50	18.92376856595889	27.125071390479615	27.889052986812125	26.062107056749372
51	21.11650571537695	23.572456459197756	27.82460940687075	27.48642841855455
52	22.96444015083345	21.45480494879396	30.75448651473748	24.82626838563511
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	283.0
1	693.5
2	1104.0
3	3740.0
4	6376.0
5	4309.0
6	2242.0
7	2245.0
8	2248.0
9	2352.5
10	2457.0
11	2398.0
12	2339.0
13	2271.0
14	2220.5
15	2238.0
16	2246.5
17	2255.0
18	2327.5
19	2400.0
20	2785.5
21	3171.0
22	3769.5
23	4368.0
24	5083.5
25	5799.0
26	6883.0
27	7967.0
28	10155.5
29	12344.0
30	15021.0
31	17698.0
32	20204.5
33	22711.0
34	24351.5
35	25992.0
36	27011.0
37	28030.0
38	32950.5
39	43874.5
40	49878.0
41	65234.0
42	80590.0
43	109141.5
44	137693.0
45	154427.5
46	171162.0
47	169649.0
48	168136.0
49	153973.0
50	139810.0
51	126402.0
52	112994.0
53	107279.0
54	101564.0
55	92673.0
56	83782.0
57	79231.5
58	74681.0
59	79846.0
60	85011.0
61	87824.0
62	90637.0
63	81830.5
64	59608.5
65	46193.0
66	37206.5
67	28220.0
68	23237.0
69	18254.0
70	15879.5
71	13505.0
72	11001.0
73	8497.0
74	7554.5
75	6612.0
76	5552.5
77	4493.0
78	3419.5
79	2346.0
80	1728.0
81	1110.0
82	927.5
83	745.0
84	471.5
85	198.0
86	171.5
87	145.0
88	78.0
89	7.5
90	4.0
91	4.0
92	4.0
93	3.5
94	3.0
95	3.5
96	4.0
97	2.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1691402.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.84659184812354
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.101032900473	13.912665881075675
2	12.241140013586191	4.858898192107572
3	4.631948311004122	2.7578516277031206
4	2.3307281652520717	1.850280424187348
5	1.2987617177707826	1.2887996860282271
6	0.7991525723468741	0.9516272956647859
7	0.5585119755256598	0.7759191454382854
8	0.40261420807059634	0.639241588786609
9	0.31524293994477726	0.5630848165877863
>10	4.413322042050952	22.83936878195031
>50	2.493890083714074	34.912346657115265
>100	0.4005136817501135	11.521241571546554
>500	0.008661369700212756	1.196414738229354
>1k	0.004181350889757881	1.6071932824192776
>5k	2.986679206969916E-4	0.3250663111598185
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTGCT	5484	0.324228066420638	TruSeq Adapter, Index 21 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTGCTT	4269	0.2523941676786477	TruSeq Adapter, Index 15 (96% over 25bp)
GCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTGCT	3487	0.20616033326199212	TruSeq Adapter, Index 21 (95% over 22bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	2991	0.17683554826114667	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2912	0.17216486677915718	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.2600800992312886	0.0
2	0.0	0.0	0.0	0.9281649188070016	0.0
3	0.0	0.0	0.0	1.4577256027839627	0.0
4	0.0	0.0	0.0	1.8010502529853931	0.0
5	0.0	0.0	0.0	3.611914849338005	0.0
6	0.0	0.0	0.0	3.9121391602942412	0.0
7	0.0	0.0	0.0	4.9454239737212085	0.0
8	0.0	0.0	0.0	6.242513607054976	0.0
9	0.0	0.0	0.0	7.153473863694143	0.0
10	0.0	0.0	0.0	7.871576360912426	0.0
11	0.0	0.0	0.0	8.539956793240163	0.0
12	0.0	0.0	0.0	9.127871434466792	0.0
13	0.0	0.0	0.0	9.406220401773204	0.0
14	0.0	0.0	0.0	9.588613469772413	0.0
15	0.0	0.0	0.0	10.172153042269077	0.0
16	0.0	0.0	0.0	10.837104366673328	0.0
17	0.0	0.0	0.0	11.630706360758708	0.0
18	0.0	0.0	0.0	12.113323739714154	0.0
19	0.0	0.0	0.0	12.613086658287031	0.0
20	0.0	0.0	0.0	13.214599486106792	0.0
21	5.912255040493035E-5	0.0	0.0	13.787615244631377	0.0
22	5.912255040493035E-5	0.0	0.0	14.40763343072788	0.0
23	5.912255040493035E-5	0.0	0.0	14.882269265378662	0.0
24	5.912255040493035E-5	0.0	0.0	15.28826381900932	0.0
25	5.912255040493035E-5	0.0	0.0	15.666175161197634	0.0
26	5.912255040493035E-5	0.0	0.0	16.00695754173165	0.0
27	5.912255040493035E-5	0.0	0.0	16.40136407548294	0.0
28	5.912255040493035E-5	0.0	0.0	16.77844770196559	0.0
29	5.912255040493035E-5	0.0	0.0	17.232568011625858	0.0
30	5.912255040493035E-5	0.0	0.0	17.696325297002133	0.0
31	5.912255040493035E-5	0.0	0.0	18.144710719273125	0.0
32	5.912255040493035E-5	0.0	0.0	18.56749607721878	0.0
33	5.912255040493035E-5	0.0	0.0	18.946353380213573	0.0
34	5.912255040493035E-5	0.0	0.0	19.430448822929144	0.0
35	5.912255040493035E-5	0.0	0.0	19.860801867326632	0.0
36	5.912255040493035E-5	0.0	0.0	20.310133250404103	0.0
37	5.912255040493035E-5	0.0	0.0	20.782049447736256	0.0
38	5.912255040493035E-5	0.0	0.0	21.215654232406017	0.0
39	5.912255040493035E-5	0.0	0.0	21.78973419683789	0.0
40	5.912255040493035E-5	0.0	0.0	22.32047733182295	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTTAT	20	6.3123036E-4	46.0	46
CGTAACG	25	3.4178443E-5	46.0	23
CGGTCTA	45	3.110472E-10	46.0	30
GTAACGA	20	6.3123036E-4	46.0	24
ATTACGG	135	0.0	44.2963	1
ATAGCGG	245	0.0	44.122448	1
GTTACGG	180	0.0	43.444447	1
CACGACC	565	0.0	42.743362	26
GCGATAA	65	0.0	42.461536	8
GGGCGAT	1875	0.0	41.952	6
TACGGGT	55	4.7293724E-11	41.81818	3
CTACCGG	105	0.0	41.619045	1
CTAGCGG	535	0.0	41.271027	1
ATTGCGG	430	0.0	41.186047	1
GCGAGAC	615	0.0	41.13821	20
ACGGGAT	700	0.0	41.07143	4
ACACGAC	590	0.0	40.9322	25
CGTATCA	45	1.589251E-8	40.88889	38
AACACGT	580	0.0	40.84483	40
TTACGGG	525	0.0	40.742855	2
>>END_MODULE