FastQCFastQC Report
Thu 26 May 2016
SRR1544787_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544787_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1229043
Sequences flagged as poor quality0
Sequence length52
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTGCT63290.5149535044746196TruSeq Adapter, Index 21 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTGCTT52410.42642934380652264TruSeq Adapter, Index 15 (96% over 25bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA50960.4146315466586605No Hit
GCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTGCT41570.3382306396114701TruSeq Adapter, Index 21 (95% over 22bp)
TGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTGCTTG27680.2252158793467763TruSeq Adapter, Index 21 (95% over 24bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC21650.1761533160353218No Hit
ACCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTGC15830.12879939920735076TruSeq Adapter, Index 15 (95% over 23bp)
TCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTGCT14120.11488613498469949TruSeq Adapter, Index 21 (95% over 22bp)
GCCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTGC13810.1123638473186048TruSeq Adapter, Index 21 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTGC13120.1067497231585876TruSeq Adapter, Index 15 (95% over 23bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12630.10276288136379282No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGACC206.3116255E-446.011
AGGCGTA206.3116255E-446.027
ATCGTAC206.3116255E-446.041
ACCTAGT206.3116255E-446.044
GAACGTA206.3116255E-446.010
TTAACGT206.3116255E-446.018
ACGAGAT650.046.016
TCGACCA253.417296E-546.029
CGATACG206.3116255E-446.09
CGGTCTA551.8189894E-1245.99999630
CGAATAT800.043.12514
TACGCGG1250.042.321
CCAACGG554.7293724E-1141.818181
GTCGGCG6850.041.6350363
GGCGATA1500.041.47
GGGCGAT11300.041.3185846
TAGACGG1450.041.2413831
GTGGCGT5750.041.234
CACGACC4250.041.1294126
GCGATTG1850.041.0270278
Produced by FastQC (version 0.11.5)