##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544787_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1229043
Sequences flagged as poor quality	0
Sequence length	52
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.923305368485885	34.0	33.0	34.0	31.0	34.0
2	33.27786741391473	34.0	34.0	34.0	31.0	34.0
3	33.375502728545705	34.0	34.0	34.0	31.0	34.0
4	36.595381121734555	37.0	37.0	37.0	35.0	37.0
5	36.606746061773265	37.0	37.0	37.0	35.0	37.0
6	36.53532301148129	37.0	37.0	37.0	35.0	37.0
7	36.420940520388626	37.0	37.0	37.0	35.0	37.0
8	36.445220386918926	37.0	37.0	37.0	35.0	37.0
9	38.30231407688746	39.0	39.0	39.0	37.0	39.0
10	38.16153787947208	39.0	39.0	39.0	35.0	39.0
11	37.5652829071074	39.0	35.0	39.0	35.0	39.0
12	37.526073538517366	39.0	35.0	39.0	35.0	39.0
13	37.51273795953437	39.0	35.0	39.0	35.0	39.0
14	38.773816701287096	41.0	35.0	41.0	35.0	41.0
15	38.80834519215357	41.0	35.0	41.0	35.0	41.0
16	38.76432557689194	41.0	35.0	41.0	35.0	41.0
17	38.852350161873915	41.0	36.0	41.0	35.0	41.0
18	38.86360119214706	41.0	36.0	41.0	35.0	41.0
19	38.81956855862651	41.0	36.0	41.0	35.0	41.0
20	38.755854758539776	41.0	35.0	41.0	35.0	41.0
21	38.69589428522843	41.0	35.0	41.0	35.0	41.0
22	38.67707313739226	41.0	35.0	41.0	35.0	41.0
23	38.65241980955914	41.0	35.0	41.0	35.0	41.0
24	38.56360517898886	40.0	35.0	41.0	35.0	41.0
25	38.520205558308376	40.0	35.0	41.0	35.0	41.0
26	38.441442650908066	40.0	35.0	41.0	35.0	41.0
27	38.42858793386399	40.0	35.0	41.0	35.0	41.0
28	38.48777463441068	40.0	36.0	41.0	35.0	41.0
29	38.49316988909257	40.0	36.0	41.0	35.0	41.0
30	38.45793678496196	40.0	35.0	41.0	35.0	41.0
31	38.43233149694518	40.0	35.0	41.0	35.0	41.0
32	38.37648642073548	40.0	35.0	41.0	35.0	41.0
33	38.27971926124635	40.0	35.0	41.0	35.0	41.0
34	38.22722801399137	40.0	35.0	41.0	35.0	41.0
35	38.12614530166967	40.0	35.0	41.0	35.0	41.0
36	38.111477792070744	40.0	35.0	41.0	35.0	41.0
37	38.04680714995326	40.0	35.0	41.0	35.0	41.0
38	37.96527867617325	40.0	35.0	41.0	35.0	41.0
39	37.918789659922396	40.0	35.0	41.0	35.0	41.0
40	37.86532285688946	40.0	35.0	41.0	35.0	41.0
41	37.825919841697974	40.0	35.0	41.0	35.0	41.0
42	37.77289240490365	40.0	35.0	41.0	35.0	41.0
43	37.70850897812363	40.0	35.0	41.0	35.0	41.0
44	37.583371777879215	39.0	35.0	41.0	35.0	41.0
45	37.550305400217894	39.0	35.0	41.0	35.0	41.0
46	37.49188108145931	39.0	35.0	41.0	35.0	41.0
47	37.419535362066256	39.0	35.0	41.0	35.0	41.0
48	37.36208253087972	39.0	35.0	41.0	35.0	41.0
49	37.323764913025826	39.0	35.0	41.0	35.0	41.0
50	37.25100179570609	39.0	35.0	41.0	35.0	41.0
51	37.16808036822145	38.0	35.0	41.0	34.0	41.0
52	36.723637822273105	37.0	35.0	40.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	5.0
12	1.0
13	9.0
14	11.0
15	28.0
16	35.0
17	59.0
18	82.0
19	182.0
20	284.0
21	456.0
22	735.0
23	1077.0
24	1772.0
25	2881.0
26	4160.0
27	4967.0
28	5137.0
29	5552.0
30	6656.0
31	8390.0
32	11359.0
33	17262.0
34	60057.0
35	231296.0
36	47659.0
37	75751.0
38	130755.0
39	608003.0
40	4422.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.16528144255327	21.654083705777584	23.140687510526483	21.03994734114266
2	27.717825983305712	24.11193099020946	25.808210127717256	22.362032898767577
3	28.788008230794205	24.63738046593976	24.356918350293682	22.217692952972353
4	26.34667786236934	25.975982939571686	23.225875742345874	24.451463455713103
5	24.70873679765476	35.014641473081085	20.590573315986504	19.68604841327765
6	86.1721681015229	2.9789844618943357	7.463286475737626	3.385560960845145
7	87.1648917084268	2.888751654742755	5.6603389791895	4.2860176576409446
8	76.25030206428904	5.251972469636945	11.327431180194672	7.170294285879339
9	51.06696836481718	20.962895521149385	13.66811413433053	14.302021979702905
10	40.42136849565068	21.672227904149814	23.682979358736837	14.223424241462668
11	38.022103376366815	18.158599821161665	23.86995410250089	19.949342699970625
12	22.79114725847672	28.481916417895874	27.12923795180478	21.59769837182263
13	17.018769888441657	30.961569286021728	30.75889126743328	21.260769558103338
14	17.897746458016524	24.490192776005397	37.795504307009594	19.81655645896848
15	20.84418527260641	18.222307925760123	40.713872500799404	20.219634300834063
16	19.136352430305532	21.687687086619427	28.579309267454434	30.596651215620607
17	22.113384153361597	23.790054538368473	29.08775364246816	25.00880766580177
18	24.67529614504944	28.06411167062503	25.755486179084052	21.505106005241476
19	29.659255209134262	23.299917089963493	23.852298088838225	23.18852961206402
20	23.910392069276664	29.307518125891445	25.548495862227767	21.233593942604124
21	21.500875071091897	23.41163002433601	27.272113343471304	27.815381561100793
22	22.956723239138093	27.284480689446994	24.035530083162264	25.723265988252646
23	23.811371937352884	21.544730330834643	32.460133616155005	22.183764115657468
24	21.979051994112492	21.488263632761427	29.73899204503016	26.793692328095926
25	19.526330649131072	28.686303082967807	27.60082438124622	24.186541886654904
26	18.901779677358725	27.357382939408954	27.191562866392793	26.549274516839528
27	21.38167663783936	27.556155480320864	30.809255656636914	20.25291222520286
28	20.803503213475853	25.047211529621016	28.531223073562114	25.618062183341024
29	21.96798647402898	24.25041271948988	29.772269969398955	24.009330837082185
30	26.673273433069472	26.90483571364061	22.3812348306772	24.040656022612716
31	28.443268461721843	25.066088005057598	22.881217337391778	23.609426195828785
32	25.82659841844427	25.775827208649332	24.770898984006255	23.626675388900146
33	18.604231096877815	29.28034251039223	27.19546834406933	24.919958048660625
34	22.146580713612135	24.624687663491024	27.400180465614305	25.828551157282536
35	23.96108191495334	25.850844925686083	26.02154684579791	24.166526313562667
36	24.40622500595992	29.98943080103788	23.908439330438398	21.6959048625638
37	22.94337952374327	28.818682503378646	25.831317537303416	22.40662043557467
38	22.140152948269506	26.464167649138393	24.564803672450843	26.83087573014126
39	25.61448216213753	23.621386721213174	26.00421628860829	24.759914828041005
40	18.398298513558924	23.683548907564667	30.75962354449763	27.15852903437878
41	21.20633696298665	26.176138670494037	23.864177250104348	28.753347116414968
42	21.34595778992273	23.492424593769297	26.57531103468308	28.586306581624893
43	22.498887345682782	26.23992813921075	26.21014887192718	25.051035643179286
44	21.27980876177644	26.847555374384786	25.299765752703525	26.57287011113525
45	21.296651134256493	26.437154761875703	26.164991786292262	26.101202317575545
46	23.233442605344155	23.100737728460274	27.315236326149694	26.350583340045873
47	21.450592046006527	21.07102843431841	32.10310786522522	25.375271654449843
48	21.231966660238903	21.457589360176986	27.957524675702967	29.352919303881148
49	20.024034960534333	24.245124051802907	29.352024298580275	26.37881668908248
50	19.07589889043752	25.29317525912437	28.895164774544096	26.73576107589401
51	20.94011356803627	22.209719269382763	27.985269840030007	28.86489732255096
52	22.296860240040424	20.737679641802607	32.13768761548619	24.82777250267078
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	165.0
1	373.5
2	582.0
3	1931.0
4	3280.0
5	2243.0
6	1206.0
7	1277.0
8	1348.0
9	1384.0
10	1420.0
11	1422.0
12	1424.0
13	1397.5
14	1390.0
15	1409.0
16	1425.0
17	1441.0
18	1533.5
19	1626.0
20	1786.0
21	1946.0
22	2362.5
23	2779.0
24	3159.0
25	3539.0
26	4268.5
27	4998.0
28	6217.5
29	7437.0
30	9195.0
31	10953.0
32	12463.0
33	13973.0
34	15261.0
35	16549.0
36	17430.5
37	18312.0
38	21920.0
39	29340.5
40	33153.0
41	44426.0
42	55699.0
43	77504.5
44	99310.0
45	117162.5
46	135015.0
47	135609.0
48	136203.0
49	122955.5
50	109708.0
51	98119.5
52	86531.0
53	81123.0
54	75715.0
55	67997.0
56	60279.0
57	56403.0
58	52527.0
59	55109.5
60	57692.0
61	59757.5
62	61823.0
63	57764.5
64	42709.5
65	31713.0
66	25611.5
67	19510.0
68	16212.5
69	12915.0
70	11216.5
71	9518.0
72	7761.0
73	6004.0
74	5325.0
75	4646.0
76	3838.5
77	3031.0
78	2310.5
79	1590.0
80	1133.5
81	677.0
82	596.5
83	516.0
84	338.5
85	161.0
86	131.0
87	101.0
88	54.0
89	4.5
90	2.0
91	1.5
92	1.0
93	2.0
94	3.0
95	1.5
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1229043.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.144641764072247
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.16440199405696	16.239299487428045
2	11.895635201409338	5.50640430585413
3	4.560658247888438	3.1666440406721783
4	2.1894568608446843	2.0269677880856154
5	1.234684541744583	1.4288165705158038
6	0.7591255903760674	1.0541813905916357
7	0.5394614418459809	0.8739949271938585
8	0.42645100248107853	0.7896044545883237
9	0.34668459175279887	0.7221501613117949
>10	6.509274130465514	40.77883820210937
>50	1.212021893394409	17.36972046912187
>100	0.14872077576162002	5.728175840502528
>500	0.007771631987540767	1.2087374092001768
>1k	0.004592327992637727	1.7438555330648384
>5k	0.0010597679983010138	1.3626094197598855
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTGCT	6329	0.5149535044746196	TruSeq Adapter, Index 21 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTGCTT	5241	0.42642934380652264	TruSeq Adapter, Index 15 (96% over 25bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA	5096	0.4146315466586605	No Hit
GCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTGCT	4157	0.3382306396114701	TruSeq Adapter, Index 21 (95% over 22bp)
TGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTGCTTG	2768	0.2252158793467763	TruSeq Adapter, Index 21 (95% over 24bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	2165	0.1761533160353218	No Hit
ACCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTGC	1583	0.12879939920735076	TruSeq Adapter, Index 15 (95% over 23bp)
TCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTGCT	1412	0.11488613498469949	TruSeq Adapter, Index 21 (95% over 22bp)
GCCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTGC	1381	0.1123638473186048	TruSeq Adapter, Index 21 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTGC	1312	0.1067497231585876	TruSeq Adapter, Index 15 (95% over 23bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1263	0.10276288136379282	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.43611167387959576	0.0
2	0.0	0.0	0.0	1.5108503119907115	0.0
3	0.0	0.0	0.0	2.2990245255861677	0.0
4	0.0	0.0	0.0	2.80616707470772	0.0
5	0.0	0.0	0.0	5.398753338980003	0.0
6	0.0	0.0	0.0	5.8192431021534645	0.0
7	0.0	0.0	0.0	7.281600399660549	0.0
8	0.0	0.0	0.0	9.058592742483379	0.0
9	0.0	0.0	0.0	10.34097261039687	0.0
10	0.0	0.0	0.0	11.332231663172077	0.0
11	0.0	0.0	0.0	12.243916608287911	0.0
12	0.0	0.0	0.0	13.054628682641697	0.0
13	0.0	0.0	0.0	13.430205452535022	0.0
14	8.13641182611186E-5	0.0	0.0	13.687478794476679	0.0
15	8.13641182611186E-5	0.0	0.0	14.441480078402464	0.0
16	8.13641182611186E-5	0.0	0.0	15.283924158878087	0.0
17	8.13641182611186E-5	0.0	0.0	16.23629116312448	0.0
18	8.13641182611186E-5	0.0	0.0	16.846847506555914	0.0
19	8.13641182611186E-5	0.0	0.0	17.456590208804737	0.0
20	8.13641182611186E-5	0.0	0.0	18.200583706184407	0.0
21	8.13641182611186E-5	0.0	0.0	18.866874470624705	0.0
22	8.13641182611186E-5	0.0	0.0	19.610867968004374	0.0
23	8.13641182611186E-5	0.0	0.0	20.163737151588673	0.0
24	1.627282365222372E-4	0.0	0.0	20.639717243416218	0.0
25	1.627282365222372E-4	0.0	0.0	21.083151687939317	0.0
26	1.627282365222372E-4	0.0	0.0	21.497864598716237	0.0
27	1.627282365222372E-4	0.0	0.0	21.94317041795934	0.0
28	1.627282365222372E-4	0.0	0.0	22.37529525004414	0.0
29	1.627282365222372E-4	0.0	0.0	22.886180548605704	0.0
30	1.627282365222372E-4	0.0	0.0	23.3980422165864	0.0
31	1.627282365222372E-4	0.0	0.0	23.890132403829647	0.0
32	1.627282365222372E-4	0.0	0.0	24.358301540304122	0.0
33	1.627282365222372E-4	0.0	0.0	24.78359178645499	0.0
34	1.627282365222372E-4	0.0	0.0	25.290083422630453	0.0
35	1.627282365222372E-4	0.0	0.0	25.753696168482307	0.0
36	1.627282365222372E-4	0.0	0.0	26.223573951440265	0.0
37	1.627282365222372E-4	0.0	0.0	26.73226241880878	0.0
38	1.627282365222372E-4	0.0	0.0	27.19628198525194	0.0
39	1.627282365222372E-4	0.0	0.0	27.798213732147694	0.0
40	1.627282365222372E-4	0.0	0.0	28.360602517568548	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCCGACC	20	6.3116255E-4	46.0	11
AGGCGTA	20	6.3116255E-4	46.0	27
ATCGTAC	20	6.3116255E-4	46.0	41
ACCTAGT	20	6.3116255E-4	46.0	44
GAACGTA	20	6.3116255E-4	46.0	10
TTAACGT	20	6.3116255E-4	46.0	18
ACGAGAT	65	0.0	46.0	16
TCGACCA	25	3.417296E-5	46.0	29
CGATACG	20	6.3116255E-4	46.0	9
CGGTCTA	55	1.8189894E-12	45.999996	30
CGAATAT	80	0.0	43.125	14
TACGCGG	125	0.0	42.32	1
CCAACGG	55	4.7293724E-11	41.81818	1
GTCGGCG	685	0.0	41.635036	3
GGCGATA	150	0.0	41.4	7
GGGCGAT	1130	0.0	41.318584	6
TAGACGG	145	0.0	41.241383	1
GTGGCGT	575	0.0	41.2	34
CACGACC	425	0.0	41.12941	26
GCGATTG	185	0.0	41.027027	8
>>END_MODULE