SRR1544786_1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1544786_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2176283
Sequences flagged as poor quality	0
Sequence length	52
%GC	50

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTGCT	7771	0.357076722099102	TruSeq Adapter, Index 22 (96% over 25bp)
CTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTGCTT	7454	0.34251060179213827	Illumina Single End Adapter 2 (95% over 24bp)
GCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTGCT	5237	0.24063965945605423	TruSeq Adapter, Index 22 (96% over 25bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4511	0.207280027459664	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	4013	0.18439697410676828	No Hit
ACCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTGC	2335	0.10729303128315573	Illumina Single End Adapter 2 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTGCT	2311	0.10619023353120895	TruSeq Adapter, Index 22 (96% over 25bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCGTAA	40	5.6152203E-9	46.000004	13
TATCGAC	20	6.3127023E-4	46.000004	33
AATCGTA	40	5.6152203E-9	46.000004	12
GTACGTC	20	6.3127023E-4	46.000004	41
CTCGTTA	30	1.8621758E-6	46.0	27
TTCGACA	25	3.4181692E-5	46.0	45
ATCGACC	25	3.4181692E-5	46.0	34
GTACGAG	25	3.4181692E-5	46.0	10
GTTACGG	225	0.0	44.97778	1
CGTAGGG	895	0.0	43.944134	2
ACGTAGG	330	0.0	43.909092	1
GCGATAT	105	0.0	43.809525	8
CTAGCGG	545	0.0	43.467888	1
TACGGGA	435	0.0	43.35632	3
CTTGCGG	760	0.0	43.276314	1
CATGCGG	465	0.0	42.537632	1
ATAGCGG	330	0.0	42.515152	1
ATCGCGG	395	0.0	42.50633	1
CGAATAT	240	0.0	42.166668	14
GGCGATA	535	0.0	42.13084	7