##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544786_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2176283
Sequences flagged as poor quality	0
Sequence length	52
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.88857699113581	34.0	33.0	34.0	31.0	34.0
2	33.23148230262333	34.0	33.0	34.0	31.0	34.0
3	33.28803239284597	34.0	34.0	34.0	31.0	34.0
4	36.581506633098726	37.0	37.0	37.0	35.0	37.0
5	36.58052330510324	37.0	37.0	37.0	35.0	37.0
6	36.688254238993736	37.0	37.0	37.0	35.0	37.0
7	36.66885556703793	37.0	37.0	37.0	35.0	37.0
8	36.56764906034739	37.0	37.0	37.0	35.0	37.0
9	38.346330877004505	39.0	39.0	39.0	37.0	39.0
10	38.18023161509785	39.0	39.0	39.0	35.0	39.0
11	37.789460745684266	39.0	37.0	39.0	35.0	39.0
12	37.74444729844418	39.0	37.0	39.0	35.0	39.0
13	37.735215043264134	39.0	37.0	39.0	35.0	39.0
14	39.18448381942974	41.0	38.0	41.0	35.0	41.0
15	39.22519497694004	41.0	38.0	41.0	35.0	41.0
16	39.189825496040726	41.0	38.0	41.0	35.0	41.0
17	39.21224583383687	41.0	38.0	41.0	35.0	41.0
18	39.21266535648167	41.0	38.0	41.0	35.0	41.0
19	39.161603982570284	41.0	38.0	41.0	35.0	41.0
20	39.08767196178071	41.0	38.0	41.0	35.0	41.0
21	39.03864892571416	41.0	38.0	41.0	35.0	41.0
22	38.99713778033463	41.0	38.0	41.0	35.0	41.0
23	38.959470804118766	41.0	37.0	41.0	35.0	41.0
24	38.917434910808936	41.0	37.0	41.0	35.0	41.0
25	38.88308046334048	41.0	37.0	41.0	35.0	41.0
26	38.811671551907544	41.0	37.0	41.0	35.0	41.0
27	38.76846439548533	41.0	37.0	41.0	35.0	41.0
28	38.77412358594907	41.0	37.0	41.0	35.0	41.0
29	38.755351211216556	41.0	37.0	41.0	35.0	41.0
30	38.71879576323484	41.0	36.0	41.0	35.0	41.0
31	38.6456798127817	41.0	36.0	41.0	35.0	41.0
32	38.560677540558835	40.0	36.0	41.0	35.0	41.0
33	38.457555382273355	40.0	36.0	41.0	35.0	41.0
34	38.33987399616686	40.0	36.0	41.0	35.0	41.0
35	38.305599042036356	40.0	36.0	41.0	35.0	41.0
36	38.2408795179671	40.0	36.0	41.0	35.0	41.0
37	38.15290704379899	40.0	35.0	41.0	35.0	41.0
38	38.07281360006947	40.0	35.0	41.0	35.0	41.0
39	38.000748983473194	40.0	35.0	41.0	35.0	41.0
40	37.928606252036154	40.0	35.0	41.0	35.0	41.0
41	37.87718325236194	40.0	35.0	41.0	35.0	41.0
42	37.82980384444486	40.0	35.0	41.0	35.0	41.0
43	37.86289880498079	40.0	35.0	41.0	35.0	41.0
44	37.80220679020146	40.0	35.0	41.0	35.0	41.0
45	37.68534193393047	40.0	35.0	41.0	35.0	41.0
46	37.55575354859639	39.0	35.0	41.0	34.0	41.0
47	37.551174640430496	39.0	35.0	41.0	34.0	41.0
48	37.56092704855021	39.0	35.0	41.0	34.0	41.0
49	37.5437514330627	39.0	35.0	41.0	34.0	41.0
50	37.48044578761126	39.0	35.0	41.0	34.0	41.0
51	37.404957443494254	39.0	35.0	41.0	34.0	41.0
52	37.16158330511243	39.0	35.0	41.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	0.0
12	4.0
13	2.0
14	5.0
15	7.0
16	29.0
17	70.0
18	129.0
19	273.0
20	451.0
21	731.0
22	1318.0
23	2089.0
24	3631.0
25	6031.0
26	8766.0
27	10973.0
28	12237.0
29	12273.0
30	14027.0
31	17252.0
32	22492.0
33	31438.0
34	88340.0
35	296590.0
36	84344.0
37	128660.0
38	228738.0
39	1196026.0
40	9356.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.3190044677094	22.016438119490893	25.03654166300982	20.628015749789892
2	26.31560325564276	23.64145655689081	27.446430450451526	22.596509737014898
3	26.63086556298055	24.764472267623283	26.421701589361312	22.18296058003486
4	26.148851045567145	26.845911124610172	23.564076914629208	23.441160915193475
5	25.711361987388585	35.327344835207555	19.755564878280996	19.205728299122864
6	88.43284627964286	2.600718748434831	6.564173868931568	2.4022611029907415
7	89.18711399206812	2.1284456111636216	5.060876733402779	3.623563663365472
8	80.0496534687814	4.414131801792322	9.530470072136758	6.005744657289516
9	50.85078549067378	22.736151502355163	13.200075541646008	13.212987465325051
10	32.16998892147758	27.7854488593625	20.641616922063903	19.40294529709601
11	30.131007777940646	18.27992958636354	25.928659094428436	25.660403541267378
12	19.874529185772253	25.286968652514403	35.06855496275071	19.769947198962633
13	13.492041246473917	25.93293243571723	37.282467399690205	23.292558918118644
14	13.201821638086592	20.938958765932554	42.57920500229061	23.28001459369025
15	20.311099245824188	15.10005821853132	45.734998619205314	18.853843916439175
16	23.89519193965123	20.885519024869467	26.679296764253547	28.539992271225756
17	18.407808175683034	24.73602927560432	31.659899011295867	25.19626353741678
18	21.29415154187208	23.73216167198843	36.50026214421562	18.473424641923867
19	24.08294325692017	19.525309897655774	31.136161978933806	25.25558486649025
20	19.828441429722147	23.21233038166452	29.372099124975932	27.587129063637406
21	22.50925086489211	20.550268508277647	32.14513002215245	24.795350604677793
22	25.799631757450666	27.816327196417014	22.441382853241056	23.942658192891276
23	19.623321047860042	27.148904806957553	29.12654282554245	24.101231319639954
24	19.233390142734194	23.54468605415748	27.666208852433254	29.55571495067507
25	19.183948043521916	32.10487790420639	26.360955813191573	22.350218239080117
26	21.290291749740266	28.533375484714075	27.084161388937012	23.09217137660865
27	21.814672080790963	29.315994289345642	28.46775901847324	20.401574611390156
28	20.32851426032368	23.46698476255156	29.388687041161464	26.81581393596329
29	19.090899483201405	21.817061475920184	34.512423246425215	24.579615794453204
30	25.731304246736293	23.544088705375174	24.6922849647771	26.03232208311143
31	28.836001567810804	27.4890719635268	23.995684384797382	19.679242083865013
32	32.07252917014929	21.46324719717059	25.28485495682317	21.179368675856956
33	29.08247686537091	25.058965217299406	24.54492361517321	21.313634302156476
34	20.592634321914936	28.322327564935257	29.355373359071407	21.7296647540784
35	29.46909937724092	25.740448278096185	24.153062813981453	20.637389530681443
36	28.407380841554154	27.584326119351203	23.105956348507984	20.902336690586658
37	21.852075304544492	32.622503599026416	23.860683559996563	21.664737536432533
38	20.171871029640904	30.411669805811105	23.288377476642513	26.12808168790548
39	23.32573475048971	24.486475334320033	27.01661502663027	25.17117488855999
40	17.35243072706996	20.858316680321447	28.748375096437368	33.04087749617122
41	19.739574310877767	27.354760387320948	21.73807358693699	31.16759171486429
42	20.554955398723422	26.07137950349288	26.205047781010098	27.168617316773602
43	23.636310167381723	26.643961286284917	27.46209936851044	22.25762917782292
44	25.469343830742602	26.054837537213682	23.574967042429684	24.900851589614035
45	25.31522784490804	22.063398923761294	26.64115834199872	25.980214889331947
46	24.75022779666064	19.921857589293303	29.240544543149948	26.08737007089611
47	24.968581751546097	17.570233283079453	28.224592114168974	29.236592851205472
48	19.34040747457936	19.22916275135173	27.635468365097736	33.79496140897117
49	20.252926664408992	22.189485466733878	31.507758871433538	26.049828997423592
50	20.669600415019556	22.208416828142298	28.500475351780995	28.621507405057155
51	18.932831805422364	22.4528243799175	30.420032688763364	28.19431112589677
52	19.233987491516498	21.494447183569417	31.626815078737465	27.644750246176624
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	277.0
1	670.0
2	1063.0
3	4545.5
4	8028.0
5	5378.5
6	2729.0
7	2976.0
8	3223.0
9	3334.0
10	3445.0
11	3365.5
12	3286.0
13	3065.5
14	2856.0
15	2867.0
16	2820.5
17	2774.0
18	3225.0
19	3676.0
20	3552.5
21	3429.0
22	3494.0
23	3559.0
24	4243.0
25	4927.0
26	5922.5
27	6918.0
28	9123.0
29	11328.0
30	12390.5
31	13453.0
32	16073.0
33	18693.0
34	21594.5
35	24496.0
36	27807.0
37	31118.0
38	33955.0
39	44771.0
40	52750.0
41	70316.0
42	87882.0
43	113889.5
44	139897.0
45	165696.5
46	191496.0
47	210448.5
48	229401.0
49	253155.0
50	276909.0
51	268915.0
52	260921.0
53	223371.0
54	185821.0
55	154619.0
56	123417.0
57	106898.5
58	90380.0
59	86922.5
60	83465.0
61	83047.5
62	82630.0
63	77345.0
64	57298.0
65	42536.0
66	33779.0
67	25022.0
68	20724.5
69	16427.0
70	13138.5
71	9850.0
72	8126.0
73	6402.0
74	5744.0
75	5086.0
76	3857.5
77	2629.0
78	2010.5
79	1392.0
80	986.5
81	581.0
82	424.5
83	268.0
84	162.5
85	57.0
86	36.5
87	16.0
88	20.5
89	21.5
90	18.0
91	17.0
92	16.0
93	9.5
94	3.0
95	1.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	2176283.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.51103905649217
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.06526038388799	14.641544745081237
2	13.158033027594382	5.660859247263934
3	5.362332013434698	3.4604800012461627
4	2.617082010340955	2.2518461335394933
5	1.4618701089839525	1.5723172504936138
6	0.9103685548124342	1.1749784123023561
7	0.6359773196551816	0.9576373073502071
8	0.4558227027260298	0.7844175968940359
9	0.34736740548581535	0.6725010443721954
>10	4.931179634423402	28.947593386015257
>50	1.694928850531526	23.79483062190598
>100	0.3473274485848442	12.356900945437097
>500	0.007942585567869944	1.230143532191866
>1k	0.0038639605465313237	1.5490850253168367
>5k	6.439934244218872E-4	0.9448647505897132
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTGCT	7771	0.357076722099102	TruSeq Adapter, Index 22 (96% over 25bp)
CTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTGCTT	7454	0.34251060179213827	Illumina Single End Adapter 2 (95% over 24bp)
GCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTGCT	5237	0.24063965945605423	TruSeq Adapter, Index 22 (96% over 25bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4511	0.207280027459664	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	4013	0.18439697410676828	No Hit
ACCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTGC	2335	0.10729303128315573	Illumina Single End Adapter 2 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTGCT	2311	0.10619023353120895	TruSeq Adapter, Index 22 (96% over 25bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.3656234046766896	0.0
2	0.0	0.0	0.0	1.192078419948141	0.0
3	0.0	0.0	0.0	1.867358243390221	0.0
4	0.0	0.0	0.0	2.298506214495082	0.0
5	0.0	0.0	0.0	4.458151812057531	0.0
6	0.0	0.0	0.0	4.862602887584013	0.0
7	0.0	0.0	0.0	6.293804620079282	0.0
8	0.0	0.0	0.0	8.195395543686184	0.0
9	0.0	0.0	0.0	9.510941361946033	0.0
10	0.0	0.0	0.0	10.54600895196075	0.0
11	0.0	0.0	0.0	11.436793836095765	0.0
12	0.0	0.0	0.0	12.233059762907674	0.0
13	4.594990633111594E-5	0.0	0.0	12.536696743943688	0.0
14	4.594990633111594E-5	0.0	0.0	12.757348194145706	0.0
15	4.594990633111594E-5	0.0	0.0	13.450778230588577	0.0
16	4.594990633111594E-5	0.0	0.0	14.224712502923563	0.0
17	4.594990633111594E-5	0.0	0.0	15.147294722239709	0.0
18	4.594990633111594E-5	0.0	0.0	15.68302468015419	0.0
19	4.594990633111594E-5	0.0	0.0	16.313227645485444	0.0
20	4.594990633111594E-5	0.0	0.0	17.18191062467519	0.0
21	4.594990633111594E-5	0.0	0.0	17.937373034665068	0.0
22	4.594990633111594E-5	0.0	0.0	18.69292734446761	0.0
23	4.594990633111594E-5	0.0	0.0	19.24970235948174	0.0
24	4.594990633111594E-5	0.0	0.0	19.765490058048517	0.0
25	4.594990633111594E-5	0.0	0.0	20.293454481793038	0.0
26	4.594990633111594E-5	0.0	0.0	20.74155796833408	0.0
27	4.594990633111594E-5	0.0	0.0	21.200781332207253	0.0
28	9.189981266223188E-5	0.0	0.0	21.6584883491715	0.0
29	9.189981266223188E-5	0.0	0.0	22.244349654893227	0.0
30	9.189981266223188E-5	0.0	0.0	22.849877520524675	0.0
31	1.3784971899334782E-4	0.0	0.0	23.432889932053875	0.0
32	1.3784971899334782E-4	0.0	0.0	23.991181293976933	0.0
33	1.3784971899334782E-4	0.0	0.0	24.44204177489784	0.0
34	1.3784971899334782E-4	0.0	0.0	25.057632670015803	0.0
35	1.3784971899334782E-4	0.0	0.0	25.638485435947437	0.0
36	1.3784971899334782E-4	0.0	0.0	26.270756147063594	0.0
37	1.3784971899334782E-4	0.0	0.0	26.890987982720997	0.0
38	1.3784971899334782E-4	0.0	0.0	27.398596597960836	0.0
39	1.3784971899334782E-4	0.0	0.0	27.9471465797417	0.0
40	1.3784971899334782E-4	0.0	0.0	28.56820551371306	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCGTAA	40	5.6152203E-9	46.000004	13
TATCGAC	20	6.3127023E-4	46.000004	33
AATCGTA	40	5.6152203E-9	46.000004	12
GTACGTC	20	6.3127023E-4	46.000004	41
CTCGTTA	30	1.8621758E-6	46.0	27
TTCGACA	25	3.4181692E-5	46.0	45
ATCGACC	25	3.4181692E-5	46.0	34
GTACGAG	25	3.4181692E-5	46.0	10
GTTACGG	225	0.0	44.97778	1
CGTAGGG	895	0.0	43.944134	2
ACGTAGG	330	0.0	43.909092	1
GCGATAT	105	0.0	43.809525	8
CTAGCGG	545	0.0	43.467888	1
TACGGGA	435	0.0	43.35632	3
CTTGCGG	760	0.0	43.276314	1
CATGCGG	465	0.0	42.537632	1
ATAGCGG	330	0.0	42.515152	1
ATCGCGG	395	0.0	42.50633	1
CGAATAT	240	0.0	42.166668	14
GGCGATA	535	0.0	42.13084	7
>>END_MODULE