SRR1544785_1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1544785_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1685111
Sequences flagged as poor quality	0
Sequence length	52
%GC	50

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTGCT	10781	0.639779812724503	TruSeq Adapter, Index 22 (96% over 25bp)
CTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTGCTT	9207	0.546373502991791	Illumina Single End Adapter 2 (95% over 24bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA	6311	0.37451538800708084	No Hit
GCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTGCT	6094	0.36163789803757734	TruSeq Adapter, Index 22 (96% over 25bp)
TGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTGCTTG	4373	0.25950812735778234	TruSeq Adapter, Index 22 (96% over 27bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	2793	0.16574575799457722	No Hit
ACCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTGC	2591	0.1537584171013067	Illumina Single End Adapter 2 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTGCT	2438	0.14467889652373048	TruSeq Adapter, Index 22 (96% over 25bp)
CGCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTGC	2218	0.1316233767389804	Illumina Single End Adapter 2 (95% over 22bp)
GCCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTGC	2201	0.1306145411192497	TruSeq Adapter, Index 22 (95% over 24bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2019	0.11981406566095645	No Hit
AGCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTGC	1773	0.1052156208107359	Illumina Single End Adapter 2 (95% over 22bp)
ACTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTGCT	1708	0.10135830814705973	Illumina Single End Adapter 2 (95% over 23bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACCCGA	20	6.312297E-4	46.0	14
ATACGCA	20	6.312297E-4	46.0	35
GCGTACC	20	6.312297E-4	46.0	33
GTACGAC	20	6.312297E-4	46.0	37
ACGTAGG	240	0.0	45.041668	1
TCGGCGT	955	0.0	44.554974	4
CTAGCGG	440	0.0	43.909092	1
ATAGCGG	305	0.0	43.737705	1
TACGGGA	385	0.0	43.61039	3
GTCGGCG	990	0.0	43.444443	3
ACGGGAT	355	0.0	43.40845	4
CGACGGT	85	0.0	43.294117	27
CTATAGG	315	0.0	43.079365	1
GTTACGG	155	0.0	43.03226	1
ACGGGAC	615	0.0	43.00813	4
CGTTTTT	2115	0.0	42.73759	1
CGTAGGG	665	0.0	42.54135	2
CTTGCGG	455	0.0	42.46154	1
CTCGAGG	195	0.0	42.46154	1
AAGACGG	130	0.0	42.461536	1