FastQCFastQC Report
Thu 26 May 2016
SRR1544784_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544784_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences932474
Sequences flagged as poor quality0
Sequence length52
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCTGCT42600.45684919901251936Illumina Single End Adapter 1 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCTGCTT32950.3533610588606224Illumina Single End Adapter 1 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCTGCT23740.25459154893326785Illumina Single End Adapter 1 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18290.1961448791065488No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC12470.13373027022737363No Hit
CGCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCTGC11590.12429300977828872Illumina Single End Adapter 1 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCTGCT10060.10788504558840246Illumina Single End Adapter 1 (95% over 22bp)
ACCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCTGC9940.10659814643625452Illumina Single End Adapter 1 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCGA206.3108373E-446.00000437
GGTCGTA351.0192889E-746.00000426
CGAGTAG206.3108373E-446.00000411
ACGTTCG206.3108373E-446.00000437
ATTCGCT206.3108373E-446.00000411
CGCGATA206.3108373E-446.00000431
CCCGTGC351.0192889E-746.00000427
TACGACC206.3108373E-446.00000418
CGCTAAA206.3108373E-446.00000418
TCGTATA351.0192889E-746.00000428
GCGATAT351.0192889E-746.0000048
GTATCCG206.3108373E-446.0000048
CGTAAAC206.3108373E-446.00000415
TCGAGTA206.3108373E-446.00000410
ACATCGG405.6097633E-946.0000041
TCGAATC206.3108373E-446.00000435
AGTCGAA206.3108373E-446.00000433
CAGACTA351.0192889E-746.00000414
ACAGTCG253.4166565E-546.010
CATGCTC301.8610226E-646.026
Produced by FastQC (version 0.11.5)