FastQCFastQC Report
Thu 26 May 2016
SRR1544783_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544783_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences698396
Sequences flagged as poor quality0
Sequence length52
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCTGCT50790.7272378421411348Illumina Single End Adapter 1 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCTGCTT38500.5512631802014902Illumina Single End Adapter 1 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCTGCT30960.4433015080269646Illumina Single End Adapter 1 (95% over 22bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA25760.36884518238936076No Hit
TGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCTGCTTG20480.29324337481887064Illumina Single End Adapter 1 (95% over 24bp)
TCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCTGCT11410.1633743606778962Illumina Single End Adapter 1 (95% over 22bp)
CGCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCTGC10990.1573605805302436Illumina Single End Adapter 1 (95% over 21bp)
ACCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCTGC10850.15535598714769272Illumina Single End Adapter 1 (95% over 21bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC9540.13659872049668098No Hit
GCCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCTGC9250.13244634848996845Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7960.11397545232217825No Hit
AGCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCTGC7730.11068219176513039Illumina Single End Adapter 1 (95% over 21bp)
ACTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCTGCT7500.10738893120808252Illumina Single End Adapter 1 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATTGG551.8189894E-1246.0000041
ACAACGA206.3097454E-446.012
CGGAAAG206.3097454E-446.040
ATCTCGG206.3097454E-446.01
TTTACGA206.3097454E-446.012
CGTGATA253.415768E-546.045
GAATCGG253.415768E-546.01
GTTAGAC206.3097454E-446.037
TCGATCA501.6370905E-1146.016
ACGGGAT1350.046.04
CGTTAGA206.3097454E-446.036
TACGCGG1000.046.01
CCATACC206.3097454E-446.028
CGTACAC206.3097454E-446.030
GTATACG206.3097454E-446.036
ACTCCCG206.3097454E-446.025
TAAATCG206.3097454E-446.040
CACAACG206.3097454E-446.011
TATACGC206.3097454E-446.012
CGTTTAC206.3097454E-446.045
Produced by FastQC (version 0.11.5)