##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544783_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	698396
Sequences flagged as poor quality	0
Sequence length	52
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.91695255986575	34.0	33.0	34.0	31.0	34.0
2	33.26168248386303	34.0	33.0	34.0	31.0	34.0
3	33.35153551853104	34.0	34.0	34.0	31.0	34.0
4	36.5863321095768	37.0	37.0	37.0	35.0	37.0
5	36.58994037766539	37.0	37.0	37.0	35.0	37.0
6	36.52032514504665	37.0	37.0	37.0	35.0	37.0
7	36.404236278558294	37.0	37.0	37.0	35.0	37.0
8	36.43927513903287	37.0	37.0	37.0	35.0	37.0
9	38.28652798698732	39.0	39.0	39.0	37.0	39.0
10	38.097324440575264	39.0	39.0	39.0	35.0	39.0
11	37.32970120103781	39.0	35.0	39.0	35.0	39.0
12	37.252167824557986	39.0	35.0	39.0	35.0	39.0
13	37.23636303758899	39.0	35.0	39.0	35.0	39.0
14	38.40714150711058	40.0	35.0	41.0	35.0	41.0
15	38.46353215081415	40.0	35.0	41.0	35.0	41.0
16	38.47102646636006	40.0	35.0	41.0	35.0	41.0
17	38.55737146260861	40.0	36.0	41.0	35.0	41.0
18	38.55327922840337	40.0	36.0	41.0	35.0	41.0
19	38.48371411061919	40.0	35.0	41.0	35.0	41.0
20	38.40005526950326	40.0	35.0	41.0	35.0	41.0
21	38.326586922032774	40.0	35.0	41.0	35.0	41.0
22	38.313685931763644	40.0	35.0	41.0	35.0	41.0
23	38.28809300167813	40.0	35.0	41.0	35.0	41.0
24	38.21416073402482	40.0	35.0	41.0	35.0	41.0
25	38.16459429893642	40.0	35.0	41.0	35.0	41.0
26	38.09884506784117	40.0	35.0	41.0	35.0	41.0
27	38.1171155046707	40.0	35.0	41.0	35.0	41.0
28	38.180201490272	40.0	35.0	41.0	35.0	41.0
29	38.211636378215225	40.0	35.0	41.0	35.0	41.0
30	38.15368644723051	40.0	35.0	41.0	35.0	41.0
31	38.11315500088775	40.0	35.0	41.0	35.0	41.0
32	38.06627185722713	40.0	35.0	41.0	35.0	41.0
33	37.989142263128656	40.0	35.0	41.0	35.0	41.0
34	37.91021712610038	40.0	35.0	41.0	35.0	41.0
35	37.8630089519413	40.0	35.0	41.0	35.0	41.0
36	37.767850904071615	40.0	35.0	41.0	35.0	41.0
37	37.73706464527288	40.0	35.0	41.0	35.0	41.0
38	37.66387837272837	40.0	35.0	41.0	35.0	41.0
39	37.59866322258432	39.0	35.0	41.0	35.0	41.0
40	37.56346685834398	39.0	35.0	41.0	35.0	41.0
41	37.52340076403645	39.0	35.0	41.0	35.0	41.0
42	37.47065275287945	39.0	35.0	41.0	35.0	41.0
43	37.404280665983194	39.0	35.0	41.0	35.0	41.0
44	37.28241284314343	39.0	35.0	41.0	35.0	41.0
45	37.268980635627926	39.0	35.0	41.0	35.0	41.0
46	37.212176186576094	38.0	35.0	41.0	35.0	41.0
47	37.13621498404916	38.0	35.0	41.0	35.0	41.0
48	37.101223374704325	38.0	35.0	41.0	34.0	41.0
49	37.054267206570486	37.0	35.0	41.0	34.0	41.0
50	36.98116541331852	37.0	35.0	41.0	34.0	41.0
51	36.89947393742232	37.0	35.0	41.0	34.0	41.0
52	36.513543892003966	36.0	35.0	40.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	3.0
12	1.0
13	5.0
14	8.0
15	21.0
16	17.0
17	28.0
18	62.0
19	99.0
20	174.0
21	286.0
22	447.0
23	692.0
24	1110.0
25	1755.0
26	2531.0
27	2971.0
28	3047.0
29	3309.0
30	4046.0
31	5114.0
32	6911.0
33	10416.0
34	37012.0
35	166571.0
36	25267.0
37	41254.0
38	69010.0
39	313377.0
40	2851.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.899667237498498	21.673806837381658	24.000423828315167	22.42610209680468
2	27.434578663108034	24.03450191581853	26.21678818320838	22.314131237865052
3	27.84237023121553	24.79367006683887	24.931700639751657	22.43225906219394
4	26.600667815966872	25.904071615530444	23.40735055756333	24.087910010939353
5	24.95790353896643	34.50578182005624	20.32514504664975	20.211169594327576
6	84.86947233374762	2.9889919186249636	8.328512763532437	3.8130229840949834
7	86.3160728297413	2.8072898470208876	6.301009742323839	4.57562758091398
8	75.6045280900807	4.994587597867112	11.819082583519952	7.581801728532237
9	55.058448215625525	17.114072818286473	13.669465460856017	14.15801350523199
10	45.59920159909277	18.98092199840778	22.2549957330798	13.16488066941964
11	41.68566257538703	17.584579522219485	22.075011884375055	18.654746018018432
12	21.825869563972304	33.26622718343175	26.29038539739632	18.61751785519963
13	15.853613136386807	36.092703852828485	28.6631080361285	19.390574974656214
14	14.833418289910021	23.856379475254727	42.59861167589734	18.71159055893791
15	16.585576091501096	17.54878321181679	45.51873149330753	20.34690920337459
16	16.718595180957507	20.64344583875051	26.39548336473863	36.24247561555335
17	20.013860331387924	23.28936591847605	31.374177400786945	25.32259634934908
18	25.59207097406056	26.436577529080925	26.1227154794701	21.848636017388415
19	30.095533193202712	21.473777054851404	23.555547282630485	24.875142469315403
20	22.117681086375065	30.507333948075306	26.04711367189961	21.327871293650023
21	21.800525776207195	22.749414372361812	25.788091569825717	29.661968281605276
22	20.411915303065882	29.12559636653131	22.913075103522928	27.549413226879878
23	20.67165333134783	21.67409320786488	36.10043585587546	21.553817604911828
24	20.115521852931575	20.99210190207275	33.04572191135116	25.846654333644526
25	17.382115590581847	30.74688285729013	27.974530209222277	23.896471342905745
26	18.29320328295122	28.385615037886815	27.768486646544368	25.5526950326176
27	20.80180871597203	24.91695255986575	34.203947330740725	20.077291393421497
28	18.775594361937927	23.30039118208008	31.521515014404432	26.40249944157756
29	20.668216885549175	25.28364996363095	29.210218844323276	24.8379143064966
30	27.620719477202044	23.619980641355333	22.390305786401985	26.368994095040637
31	31.009055034679466	24.584762799328747	23.368976912811643	21.037205253180144
32	25.94373392745663	24.55082789706699	24.52791825840927	24.97751991706711
33	19.877691166616074	23.03793263420753	30.27623296811551	26.80814323106089
34	20.58116025864982	23.56743165768418	29.414114628377025	26.437293455288973
35	30.32964106323633	20.368100619132985	25.5806161547317	23.721642162898984
36	22.775617271576586	26.4375798257722	28.217945119960596	22.56885778269062
37	23.482952365133823	27.56187034290002	26.143620524745277	22.81155676722089
38	21.982657403536102	25.818446841047198	26.297258288993635	25.90163746642306
39	27.102675273054256	20.696281192904888	27.87057772381285	24.33046581022801
40	17.500529785393958	20.064404721676528	31.967250671538782	30.467814821390732
41	22.94844185820079	24.30726980108706	23.88673474647621	28.857553594235934
42	22.84649396617392	20.462459693354486	27.383747902336207	29.307298438135383
43	23.954174995274887	24.120699431268218	28.188735330672	23.736390242784896
44	19.08601996575009	28.097955887490766	25.712776132738448	27.103248014020696
45	21.687695805817903	25.276204331067188	26.841219021872973	26.19488084124193
46	24.493983356147513	21.351210488032578	28.04282956947062	26.11197658634929
47	22.68856064467723	18.1122171375552	33.47427533949221	25.72494687827536
48	20.77818315110625	17.666481480420853	31.147515163317085	30.407820205155815
49	19.84533130201204	23.665513548187562	30.754328489853894	25.734826659946506
50	19.560249485964984	25.135453238563795	28.30213804202773	27.00215923344349
51	20.700433564911595	20.33588393977056	30.213517832289988	28.750164663027856
52	24.104376313724593	18.4298020034479	33.01937582689477	24.446445855932737
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	175.0
1	217.5
2	260.0
3	1013.0
4	1766.0
5	1226.5
6	687.0
7	730.0
8	773.0
9	858.5
10	944.0
11	923.5
12	903.0
13	818.5
14	739.5
15	745.0
16	742.0
17	739.0
18	842.0
19	945.0
20	888.0
21	831.0
22	1083.0
23	1335.0
24	1268.5
25	1202.0
26	1547.0
27	1892.0
28	2642.5
29	3393.0
30	4142.0
31	4891.0
32	5294.5
33	5698.0
34	6026.0
35	6354.0
36	7198.5
37	8043.0
38	10068.5
39	13927.0
40	15760.0
41	23154.0
42	30548.0
43	43338.0
44	56128.0
45	62228.5
46	68329.0
47	74842.0
48	81355.0
49	82160.5
50	82966.0
51	74751.0
52	66536.0
53	57036.0
54	47536.0
55	42093.0
56	36650.0
57	32673.0
58	28696.0
59	28711.5
60	28727.0
61	29340.0
62	29953.0
63	28176.0
64	20786.0
65	15173.0
66	12312.0
67	9451.0
68	7930.0
69	6409.0
70	5476.5
71	4544.0
72	3949.5
73	3355.0
74	3006.5
75	2658.0
76	1927.5
77	1197.0
78	1076.0
79	955.0
80	615.0
81	275.0
82	265.5
83	256.0
84	181.5
85	107.0
86	62.5
87	18.0
88	12.0
89	4.5
90	3.0
91	1.5
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	698396.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.29418257492387
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.02240005069854	17.71159371216727
2	12.20246213396305	6.173026101601129
3	4.597328038519573	3.4885696428939217
4	2.229532841066972	2.2557684295494687
5	1.223449034393667	1.5473071623533865
6	0.7612823984399025	1.15536095863289
7	0.5505121527176106	0.9747328430387528
8	0.4236427672154401	0.857255800039465
9	0.35189730197427094	0.8010859143384295
>10	6.964733207035612	46.46445882632657
>50	0.529692108876785	8.808013235261294
>100	0.12944244461297533	5.384238428376236
>500	0.009083680323717567	1.5102692577807881
>1k	0.003974110141626436	2.1389617376290615
>5k	5.677300202323479E-4	0.7293579500112791
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCTGCT	5079	0.7272378421411348	Illumina Single End Adapter 1 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCTGCTT	3850	0.5512631802014902	Illumina Single End Adapter 1 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCTGCT	3096	0.4433015080269646	Illumina Single End Adapter 1 (95% over 22bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA	2576	0.36884518238936076	No Hit
TGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCTGCTTG	2048	0.29324337481887064	Illumina Single End Adapter 1 (95% over 24bp)
TCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCTGCT	1141	0.1633743606778962	Illumina Single End Adapter 1 (95% over 22bp)
CGCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCTGC	1099	0.1573605805302436	Illumina Single End Adapter 1 (95% over 21bp)
ACCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCTGC	1085	0.15535598714769272	Illumina Single End Adapter 1 (95% over 21bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	954	0.13659872049668098	No Hit
GCCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCTGC	925	0.13244634848996845	Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	796	0.11397545232217825	No Hit
AGCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCTGC	773	0.11068219176513039	Illumina Single End Adapter 1 (95% over 21bp)
ACTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCTGCT	750	0.10738893120808252	Illumina Single End Adapter 1 (95% over 22bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.5595679242149153	0.0
2	0.0	0.0	0.0	2.0252120573428254	0.0
3	0.0	0.0	0.0	3.0366726040813523	0.0
4	0.0	0.0	0.0	3.674849225940584	0.0
5	0.0	0.0	0.0	6.849552402934725	0.0
6	0.0	0.0	0.0	7.373753572471778	0.0
7	0.0	0.0	0.0	9.122761298747415	0.0
8	0.0	0.0	0.0	11.180046850211054	0.0
9	0.0	0.0	0.0	12.714563084553749	0.0
10	0.0	0.0	0.0	13.846299234245327	0.0
11	0.0	0.0	0.0	14.901717650158362	0.0
12	0.0	0.0	0.0	15.88196381422574	0.0
13	0.0	0.0	0.0	16.31524235533995	0.0
14	1.431852416107767E-4	0.0	0.0	16.631538554058157	0.0
15	1.431852416107767E-4	0.0	0.0	17.593027451474523	0.0
16	1.431852416107767E-4	0.0	0.0	18.572128133609013	0.0
17	1.431852416107767E-4	0.0	0.0	19.877691166616074	0.0
18	1.431852416107767E-4	0.0	0.0	20.550948172669948	0.0
19	1.431852416107767E-4	0.0	0.0	21.249262596005703	0.0
20	1.431852416107767E-4	0.0	0.0	22.117681086375065	0.0
21	1.431852416107767E-4	0.0	0.0	22.841625667959153	0.0
22	1.431852416107767E-4	0.0	0.0	23.69901889472448	0.0
23	1.431852416107767E-4	0.0	0.0	24.287080682019944	0.0
24	1.431852416107767E-4	0.0	0.0	24.797536068362362	0.0
25	1.431852416107767E-4	0.0	0.0	25.28594092749672	0.0
26	1.431852416107767E-4	0.0	0.0	25.7227990996512	0.0
27	1.431852416107767E-4	0.0	0.0	26.18528743005401	0.0
28	1.431852416107767E-4	0.0	0.0	26.63145264291319	0.0
29	4.295557248323301E-4	0.0	0.0	27.22166220883281	0.0
30	4.295557248323301E-4	0.0	0.0	27.784809764087996	0.0
31	4.295557248323301E-4	0.0	0.0	28.273214623222355	0.0
32	4.295557248323301E-4	0.0	0.0	28.760903556148662	0.0
33	4.295557248323301E-4	0.0	0.0	29.220241811236033	0.0
34	4.295557248323301E-4	0.0	0.0	29.84868183666573	0.0
35	4.295557248323301E-4	0.0	0.0	30.328638766545055	0.0
36	4.295557248323301E-4	0.0	0.0	30.83265081701499	0.0
37	4.295557248323301E-4	0.0	0.0	31.28425706905538	0.0
38	4.295557248323301E-4	0.0	0.0	31.726413095149457	0.0
39	4.295557248323301E-4	0.0	0.0	32.25075744992812	0.0
40	4.295557248323301E-4	0.0	0.0	32.77409950801551	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATATTGG	55	1.8189894E-12	46.000004	1
ACAACGA	20	6.3097454E-4	46.0	12
CGGAAAG	20	6.3097454E-4	46.0	40
ATCTCGG	20	6.3097454E-4	46.0	1
TTTACGA	20	6.3097454E-4	46.0	12
CGTGATA	25	3.415768E-5	46.0	45
GAATCGG	25	3.415768E-5	46.0	1
GTTAGAC	20	6.3097454E-4	46.0	37
TCGATCA	50	1.6370905E-11	46.0	16
ACGGGAT	135	0.0	46.0	4
CGTTAGA	20	6.3097454E-4	46.0	36
TACGCGG	100	0.0	46.0	1
CCATACC	20	6.3097454E-4	46.0	28
CGTACAC	20	6.3097454E-4	46.0	30
GTATACG	20	6.3097454E-4	46.0	36
ACTCCCG	20	6.3097454E-4	46.0	25
TAAATCG	20	6.3097454E-4	46.0	40
CACAACG	20	6.3097454E-4	46.0	11
TATACGC	20	6.3097454E-4	46.0	12
CGTTTAC	20	6.3097454E-4	46.0	45
>>END_MODULE