FastQCFastQC Report
Thu 26 May 2016
SRR1544782_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544782_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1018747
Sequences flagged as poor quality0
Sequence length52
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTGCT42250.41472514765687657No Hit
CTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTGCTT27650.2714118421943819Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24240.23793935098704583No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC18240.1790434720298563No Hit
GCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTGCT18240.1790434720298563No Hit
CGCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTGC12030.11808623730916509No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA11380.11170585042213621No Hit
GCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAATAATT11210.1100371338516825No Hit
ACCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTGC10270.10081011281505615No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCGAT206.311117E-446.026
CACGCTA206.311117E-446.032
TACGGGT453.092282E-1046.03
AATGCGG1800.046.01
CCGTAGG253.41688E-546.01
TACGCCC253.41688E-546.017
ACTACGG551.8189894E-1246.01
GAACGAC206.311117E-446.019
CGGTCTA501.6370905E-1146.030
ACGTAGG1050.046.01
GACGCGT206.311117E-446.031
TGCACGA206.311117E-446.038
ATTTCGC253.41688E-546.030
TACGCGG1200.044.0833321
ATGACGG1050.043.8095251
TACCAGG950.043.5789451
TAACGGG2450.043.1836742
ATGGGCG800.043.1254
ATTGCGG2250.042.9333341
ATAGCGG1300.042.4615361
Produced by FastQC (version 0.11.5)