##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544782_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1018747
Sequences flagged as poor quality	0
Sequence length	52
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.87165410057649	34.0	33.0	34.0	31.0	34.0
2	33.22622299746649	34.0	33.0	34.0	31.0	34.0
3	33.28729998714107	34.0	34.0	34.0	31.0	34.0
4	36.57033394944967	37.0	37.0	37.0	35.0	37.0
5	36.564193072470395	37.0	37.0	37.0	35.0	37.0
6	36.6637241631141	37.0	37.0	37.0	35.0	37.0
7	36.641341765914405	37.0	37.0	37.0	35.0	37.0
8	36.5347451329918	37.0	37.0	37.0	35.0	37.0
9	38.3238281928683	39.0	39.0	39.0	37.0	39.0
10	38.089632656586964	39.0	39.0	39.0	35.0	39.0
11	37.34488150639953	39.0	35.0	39.0	35.0	39.0
12	37.25344859911244	39.0	35.0	39.0	35.0	39.0
13	37.2393989871872	39.0	35.0	39.0	35.0	39.0
14	38.498809812446076	41.0	35.0	41.0	35.0	41.0
15	38.55999183310478	41.0	35.0	41.0	35.0	41.0
16	38.52689136753286	41.0	35.0	41.0	35.0	41.0
17	38.602526436887665	41.0	36.0	41.0	35.0	41.0
18	38.59066677006165	40.0	36.0	41.0	35.0	41.0
19	38.50554259300886	40.0	35.0	41.0	35.0	41.0
20	38.416298649223016	40.0	35.0	41.0	35.0	41.0
21	38.350134037204526	40.0	35.0	41.0	35.0	41.0
22	38.32873520118341	40.0	35.0	41.0	35.0	41.0
23	38.27937456502939	40.0	35.0	41.0	35.0	41.0
24	38.22824214451674	40.0	35.0	41.0	35.0	41.0
25	38.17610849406182	40.0	35.0	41.0	35.0	41.0
26	38.12292158897155	40.0	35.0	41.0	35.0	41.0
27	38.106348288633	40.0	35.0	41.0	35.0	41.0
28	38.11360622411649	40.0	35.0	41.0	35.0	41.0
29	38.10864915430426	40.0	35.0	41.0	35.0	41.0
30	38.040827604891106	40.0	35.0	41.0	35.0	41.0
31	37.93073255675845	40.0	35.0	41.0	35.0	41.0
32	37.85644031344387	40.0	35.0	41.0	35.0	41.0
33	37.73938082762452	40.0	35.0	41.0	35.0	41.0
34	37.62868307833054	40.0	35.0	41.0	35.0	41.0
35	37.56747259133033	40.0	35.0	41.0	35.0	41.0
36	37.51895711103935	40.0	35.0	41.0	34.0	41.0
37	37.4509657451752	39.0	35.0	41.0	34.0	41.0
38	37.40319726094899	39.0	35.0	41.0	34.0	41.0
39	37.292536812378344	39.0	35.0	41.0	34.0	41.0
40	37.21761143836497	39.0	35.0	41.0	34.0	41.0
41	37.15906697148556	39.0	35.0	41.0	34.0	41.0
42	37.05575967340272	38.0	35.0	41.0	34.0	41.0
43	37.04155889538816	39.0	35.0	41.0	34.0	41.0
44	37.00779585117797	38.0	35.0	41.0	34.0	41.0
45	36.982583506994374	38.0	35.0	41.0	34.0	41.0
46	36.927155613709786	38.0	35.0	41.0	34.0	41.0
47	36.834754850811834	37.0	35.0	41.0	34.0	41.0
48	36.81121514959062	37.0	35.0	41.0	34.0	41.0
49	36.741085863320336	37.0	35.0	41.0	34.0	41.0
50	36.64330103548771	37.0	35.0	41.0	34.0	41.0
51	36.554191570625484	37.0	35.0	40.0	34.0	41.0
52	36.25866873718401	36.0	35.0	40.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	2.0
14	5.0
15	9.0
16	23.0
17	57.0
18	86.0
19	154.0
20	265.0
21	455.0
22	766.0
23	1286.0
24	2119.0
25	3490.0
26	4963.0
27	5956.0
28	6140.0
29	6697.0
30	7797.0
31	9463.0
32	12104.0
33	17454.0
34	56169.0
35	222007.0
36	40142.0
37	63236.0
38	109223.0
39	444863.0
40	3816.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.555031818498605	22.930128873999138	25.38324039236435	22.13159891513791
2	26.979515031700707	24.649495900356026	26.281991505251057	22.08899756269221
3	27.18604324724392	24.639581760731566	24.969693162286614	23.204681829737904
4	26.08950014085931	27.420939644484843	23.31452264399306	23.175037570662784
5	25.126454360111	34.642065203627595	20.290611898734426	19.94086853752698
6	87.50298160387221	3.0652360203269313	6.9733702283295065	2.4584121474713547
7	88.75039632018549	2.5540197909785256	5.185880301978804	3.5097035868571878
8	79.89903283150772	4.854983622037659	9.638801390335383	5.607182156119233
9	53.36948231503995	19.939788779746102	13.209560371711524	13.48116853350243
10	43.06348877591787	20.6271036871765	22.384262235864252	13.925145301041377
11	41.409986974194766	17.76535292864666	23.360755908974458	17.463904188184113
12	21.968948129417804	33.68274949521324	25.466283581693983	18.882018793674977
13	15.396069877997187	35.30317144492205	29.315816390134152	19.984942286946612
14	15.186498708707855	23.890131701001327	42.444493088077806	18.478876502213012
15	17.600444467566533	17.61124204537535	44.55797170445655	20.23034178260157
16	17.41776908300098	20.591079041214357	27.23708634234015	34.75406553344452
17	21.14038127228841	23.524093813282395	30.678470709606998	24.6570542048222
18	24.62240379603572	27.705504899646332	25.83291042820249	21.839180876115464
19	29.293926755121735	22.442765475628395	24.70760650092712	23.555701268322753
20	21.715106891112317	29.06256411061824	26.656078496427476	22.56625050184197
21	21.5638426419906	23.67182430966668	26.480372457538525	28.283960590804192
22	21.352700915929077	27.473062497361955	23.64914939626816	27.525087190440807
23	21.440259455978765	21.602910241698872	35.52677946536284	21.43005083695952
24	20.57007284438629	21.89493564152827	31.727308154036283	25.80768336004916
25	17.687414048826646	30.818446581928583	27.539320361188796	23.954819008055974
26	18.128347862619474	28.463004062834052	27.679885192299952	25.728762882246524
27	20.487029655056652	25.954824897643867	33.706896805585686	19.85124864171379
28	18.5049870085507	23.747211034731883	31.550620517164713	26.197181439552708
29	19.57944416032636	26.199537274710995	28.36366634699292	25.85735221796972
30	29.760283956664413	23.69116178992429	22.925024564489515	23.62352968892178
31	27.885039170667497	25.567584493500352	23.82249959999882	22.724876735833334
32	25.7789225391584	25.809450236417874	22.739797025169153	25.671830199254575
33	21.078246119988574	24.940539702202805	27.4910257404439	26.490188437364726
34	20.009874875705155	24.573029417509943	28.596599548268607	26.820496158516292
35	27.585160986977144	24.88478493679	23.9808313545954	23.54922272163746
36	23.00188368652865	26.876545403323888	26.845134267880056	23.276436642267413
37	22.16477692695046	27.342509965673518	25.756934744347713	24.735778363028306
38	20.63014664092262	27.636007762476844	25.792272271722027	25.941573324878505
39	25.717376345648134	22.92571168307735	26.43247047598668	24.924441495287837
40	17.550873769444227	21.534885501503318	31.22482814673319	29.689412582319264
41	21.62774467065915	24.879876946876898	24.255286150535905	29.237092231928045
42	23.023086202953237	22.970570710883077	26.973330964410202	27.033012121753487
43	22.68168642459806	26.61171026761306	27.38923402964622	23.31736927814266
44	19.690364732362404	28.974220292182455	24.467114995185263	26.86829998026988
45	22.50843438066566	26.946827818879466	24.742944028301434	25.80179377215344
46	22.798103945336774	23.87540773126203	27.435074655434565	25.891413667966628
47	23.156190889396484	20.800453890907164	31.4600190233689	24.583336196327448
48	20.374244046853633	20.94788990789666	29.35714166520245	29.320724380047253
49	20.129237190391727	25.222847282004267	30.15518082507237	24.492734702531642
50	19.20898908168564	26.771808898578353	27.70589753883938	26.313304480896633
51	21.019448400829646	21.568456152508915	28.938882764808145	28.473212681853294
52	22.578225997229932	20.738711377800374	30.79400479216135	25.889057832808344
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	244.0
1	443.0
2	642.0
3	2540.0
4	4438.0
5	2931.0
6	1424.0
7	1536.0
8	1648.0
9	1722.0
10	1796.0
11	1773.0
12	1750.0
13	1666.0
14	1554.5
15	1527.0
16	1395.5
17	1264.0
18	1389.5
19	1515.0
20	1614.0
21	1713.0
22	1840.5
23	1968.0
24	2444.0
25	2920.0
26	3394.5
27	3869.0
28	5945.5
29	8022.0
30	8734.5
31	9447.0
32	10865.0
33	12283.0
34	14360.0
35	16437.0
36	17929.5
37	19422.0
38	20966.0
39	25726.0
40	28942.0
41	38427.0
42	47912.0
43	60700.0
44	73488.0
45	79555.5
46	85623.0
47	85153.5
48	84684.0
49	89226.0
50	93768.0
51	97178.0
52	100588.0
53	90441.0
54	80294.0
55	71288.0
56	62282.0
57	56057.0
58	49832.0
59	48402.5
60	46973.0
61	45692.0
62	44411.0
63	40293.5
64	29962.0
65	23748.0
66	18987.5
67	14227.0
68	12344.5
69	10462.0
70	8672.0
71	6882.0
72	6025.0
73	5168.0
74	4083.0
75	2998.0
76	2484.0
77	1970.0
78	1612.5
79	1255.0
80	750.5
81	246.0
82	230.5
83	215.0
84	168.5
85	122.0
86	76.0
87	30.0
88	24.0
89	14.0
90	10.0
91	5.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1018747.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.282698117399285
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.42487457346397	15.692562200762064
2	12.141505804981456	5.410910170861056
3	4.611318801323656	3.082578743189477
4	2.2415142703555344	1.9978794324869962
5	1.2495636364256	1.392182464447566
6	0.7935349422911424	1.0609259738808834
7	0.5249920505448289	0.8188767563427393
8	0.3691754049352705	0.6580979280433017
9	0.29657508753854905	0.5947643830286481
>10	5.288786842392257	32.658393763055756
>50	1.84506306666805	26.550119717705943
>100	0.20206573030742153	7.282660665041302
>500	0.0070590648142330676	1.0746198481292466
>1k	0.0039707239580061005	1.7254279530250123
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTGCT	4225	0.41472514765687657	No Hit
CTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTGCTT	2765	0.2714118421943819	Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2424	0.23793935098704583	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	1824	0.1790434720298563	No Hit
GCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTGCT	1824	0.1790434720298563	No Hit
CGCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTGC	1203	0.11808623730916509	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA	1138	0.11170585042213621	No Hit
GCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAATAATT	1121	0.1100371338516825	No Hit
ACCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTGC	1027	0.10081011281505615	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.28044254363448434	0.0
2	0.0	0.0	0.0	1.0372545882343702	0.0
3	0.0	0.0	0.0	1.694041798405296	0.0
4	0.0	0.0	0.0	2.122116678625802	0.0
5	0.0	0.0	0.0	4.271619940966698	0.0
6	0.0	0.0	0.0	4.624897054911573	0.0
7	0.0	0.0	0.0	5.92885181502375	0.0
8	9.815979826198262E-5	0.0	0.0	7.54289337784553	0.0
9	9.815979826198262E-5	0.0	0.0	8.746038025142651	0.0
10	9.815979826198262E-5	0.0	0.0	9.557721396970985	0.0
11	9.815979826198262E-5	0.0	0.0	10.378926269230732	0.0
12	9.815979826198262E-5	0.0	0.0	11.099026549280635	0.0
13	9.815979826198262E-5	0.0	0.0	11.399984490751875	0.0
14	9.815979826198262E-5	0.0	0.0	11.617015804709117	0.0
15	1.9631959652396524E-4	0.0	0.0	12.33299337323202	0.0
16	1.9631959652396524E-4	0.0	0.0	13.141339311919445	0.0
17	1.9631959652396524E-4	0.0	0.0	14.195968184446187	0.0
18	1.9631959652396524E-4	0.0	0.0	14.71788383180515	0.0
19	1.9631959652396524E-4	0.0	0.0	15.30458494601702	0.0
20	3.9263919304793047E-4	0.0	0.0	16.009372297538054	0.0
21	3.9263919304793047E-4	0.0	0.0	16.619239124139753	0.0
22	4.90798991309913E-4	0.0	0.0	17.320296403326832	0.0
23	4.90798991309913E-4	0.0	0.0	17.81934081769075	0.0
24	4.90798991309913E-4	0.0	0.0	18.255857440561787	0.0
25	4.90798991309913E-4	0.0	0.0	18.677061134903955	0.0
26	4.90798991309913E-4	0.0	0.0	19.060276987318737	0.0
27	4.90798991309913E-4	0.0	0.0	19.48373835702093	0.0
28	4.90798991309913E-4	0.0	0.0	19.90268437600307	0.0
29	4.90798991309913E-4	0.0	0.0	20.43127488964385	0.0
30	4.90798991309913E-4	0.0	0.0	20.93827024766699	0.0
31	4.90798991309913E-4	0.0	0.0	21.403547691428784	0.0
32	4.90798991309913E-4	0.0	0.0	21.84929133533645	0.0
33	4.90798991309913E-4	0.0	0.0	22.265096240774206	0.0
34	4.90798991309913E-4	0.0	0.0	22.824508931069246	0.0
35	4.90798991309913E-4	0.0	0.0	23.254743326851514	0.0
36	4.90798991309913E-4	0.0	0.0	23.70755447623404	0.0
37	4.90798991309913E-4	0.0	0.0	24.17400983757498	0.0
38	4.90798991309913E-4	0.0	0.0	24.622894595027027	0.0
39	4.90798991309913E-4	0.0	0.0	25.147951355930374	0.0
40	4.90798991309913E-4	0.0	0.0	25.654161435567417	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGCGAT	20	6.311117E-4	46.0	26
CACGCTA	20	6.311117E-4	46.0	32
TACGGGT	45	3.092282E-10	46.0	3
AATGCGG	180	0.0	46.0	1
CCGTAGG	25	3.41688E-5	46.0	1
TACGCCC	25	3.41688E-5	46.0	17
ACTACGG	55	1.8189894E-12	46.0	1
GAACGAC	20	6.311117E-4	46.0	19
CGGTCTA	50	1.6370905E-11	46.0	30
ACGTAGG	105	0.0	46.0	1
GACGCGT	20	6.311117E-4	46.0	31
TGCACGA	20	6.311117E-4	46.0	38
ATTTCGC	25	3.41688E-5	46.0	30
TACGCGG	120	0.0	44.083332	1
ATGACGG	105	0.0	43.809525	1
TACCAGG	95	0.0	43.578945	1
TAACGGG	245	0.0	43.183674	2
ATGGGCG	80	0.0	43.125	4
ATTGCGG	225	0.0	42.933334	1
ATAGCGG	130	0.0	42.461536	1
>>END_MODULE