FastQCFastQC Report
Thu 26 May 2016
SRR1544781_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544781_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences860022
Sequences flagged as poor quality0
Sequence length52
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTGCT63370.7368416156795988No Hit
CTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTGCTT49620.5769619846934148Illumina Single End Adapter 1 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTGCT34750.40405943103781067No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA31940.3713858482689978No Hit
TGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTGCTTG23520.27348137605782175Illumina Single End Adapter 1 (95% over 22bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC15800.18371623051503333No Hit
ACCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTGC13970.162437705081963No Hit
TCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTGCT13880.1613912202245989No Hit
CGCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTGC13860.16115866803407355No Hit
GCCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTGC11900.13836855336258838No Hit
ACTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTGCT9810.11406684945268841No Hit
AGCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTGC9650.11220643192848556No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9470.11011346221375731No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGTA206.3105643E-446.026
CGAAACG206.3105643E-446.046
CGACGGT501.6370905E-1146.027
GCGGTAT253.416434E-546.013
CCGATAG301.8608534E-646.011
AATGCGG1200.046.01
CCCGTAT206.3105643E-446.039
CCTTTAG206.3105643E-446.044
CTTGAAC206.3105643E-446.010
AATTCGG206.3105643E-446.012
GCGATAT301.8608534E-646.08
CCGGTAT206.3105643E-446.042
ACGTAGG551.8189894E-1246.01
TGTACGC206.3105643E-446.013
CGATAGT206.3105643E-446.09
GTACGTA206.3105643E-446.046
ATGGGCG950.045.9999964
ACTAAGG1100.043.9090921
TCGAAGG1000.043.6999971
CTTAAGG850.043.2941171
Produced by FastQC (version 0.11.5)