##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544781_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	860022
Sequences flagged as poor quality	0
Sequence length	52
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.869411480171436	34.0	33.0	34.0	31.0	34.0
2	33.23182662769092	34.0	33.0	34.0	31.0	34.0
3	33.33393913178965	34.0	34.0	34.0	31.0	34.0
4	36.57511319477874	37.0	37.0	37.0	35.0	37.0
5	36.571238875284585	37.0	37.0	37.0	35.0	37.0
6	36.50202552957948	37.0	37.0	37.0	35.0	37.0
7	36.38453783740416	37.0	37.0	37.0	35.0	37.0
8	36.4208113280823	37.0	37.0	37.0	35.0	37.0
9	38.263968828704385	39.0	39.0	39.0	37.0	39.0
10	38.11604121755025	39.0	39.0	39.0	35.0	39.0
11	37.48940027115585	39.0	35.0	39.0	35.0	39.0
12	37.44770598891656	39.0	35.0	39.0	35.0	39.0
13	37.43055642762627	39.0	35.0	39.0	35.0	39.0
14	38.685969661241224	41.0	35.0	41.0	35.0	41.0
15	38.740661285408976	41.0	35.0	41.0	35.0	41.0
16	38.72087690780003	41.0	35.0	41.0	35.0	41.0
17	38.79984465513673	41.0	36.0	41.0	35.0	41.0
18	38.80683401122297	41.0	36.0	41.0	35.0	41.0
19	38.73962177711733	41.0	36.0	41.0	35.0	41.0
20	38.672743255405095	41.0	35.0	41.0	35.0	41.0
21	38.61196457765034	41.0	35.0	41.0	35.0	41.0
22	38.60181483729486	41.0	35.0	41.0	35.0	41.0
23	38.56565878547293	41.0	35.0	41.0	35.0	41.0
24	38.49851282874159	40.0	35.0	41.0	35.0	41.0
25	38.437288813541976	40.0	35.0	41.0	35.0	41.0
26	38.37361137273233	40.0	35.0	41.0	35.0	41.0
27	38.36524414491722	40.0	35.0	41.0	35.0	41.0
28	38.39990372339312	40.0	36.0	41.0	35.0	41.0
29	38.42710651587983	40.0	36.0	41.0	35.0	41.0
30	38.3945608368158	40.0	35.0	41.0	35.0	41.0
31	38.32976365720877	40.0	35.0	41.0	35.0	41.0
32	38.30470267039681	40.0	35.0	41.0	35.0	41.0
33	38.216168888702846	40.0	35.0	41.0	35.0	41.0
34	38.147304371283525	40.0	35.0	41.0	35.0	41.0
35	38.08489201439033	40.0	35.0	41.0	35.0	41.0
36	38.06774245310004	40.0	35.0	41.0	35.0	41.0
37	38.013170593310406	40.0	35.0	41.0	35.0	41.0
38	37.94802109713473	40.0	35.0	41.0	35.0	41.0
39	37.91192899716519	40.0	35.0	41.0	35.0	41.0
40	37.86060007767243	40.0	35.0	41.0	35.0	41.0
41	37.81470822839416	40.0	35.0	41.0	35.0	41.0
42	37.73995665227169	40.0	35.0	41.0	35.0	41.0
43	37.64644276541763	39.0	35.0	41.0	35.0	41.0
44	37.515121706188914	39.0	35.0	41.0	35.0	41.0
45	37.49679310529265	39.0	35.0	41.0	35.0	41.0
46	37.44062942575888	39.0	35.0	41.0	35.0	41.0
47	37.36078611942485	39.0	35.0	41.0	35.0	41.0
48	37.30369688217278	39.0	35.0	41.0	35.0	41.0
49	37.24441584052501	39.0	35.0	41.0	35.0	41.0
50	37.14853922341521	38.0	35.0	41.0	35.0	41.0
51	37.05499626753734	38.0	35.0	41.0	34.0	41.0
52	36.57848287601945	37.0	35.0	40.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	1.0
13	4.0
14	5.0
15	15.0
16	18.0
17	31.0
18	62.0
19	112.0
20	167.0
21	319.0
22	509.0
23	779.0
24	1299.0
25	2211.0
26	3008.0
27	3470.0
28	3596.0
29	4090.0
30	5076.0
31	6257.0
32	8361.0
33	12469.0
34	40448.0
35	172533.0
36	31503.0
37	51952.0
38	93018.0
39	415667.0
40	3040.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.512333405424513	22.0216459578941	23.62044226775594	22.845578368925445
2	26.901404847782963	24.329377620572497	26.430254109778588	22.33896342186595
3	27.8549851050322	24.32135456999937	24.769831469427526	23.053828855540907
4	26.217585131543146	26.79582615328445	23.515212401543216	23.471376313629186
5	24.62634676787338	34.18040468732195	20.339712239919443	20.853536304885225
6	84.76457578992165	3.085967568271509	8.385483162058645	3.7639734797481923
7	86.24314261728189	2.6523740090369783	6.191469520547148	4.9130138531339895
8	75.42644257937587	5.178123350332899	11.81144203287823	7.5839920374129965
9	50.43975619228346	19.386364534860736	14.683112757580622	15.49076651527519
10	40.125950266388536	20.372734650974046	24.94575720155996	14.55555788107746
11	38.09472315824479	17.856287397299138	24.40588729125534	19.64310215320073
12	21.208294671531657	31.153737927634413	27.59615451697747	20.041812883856462
13	15.181239549685937	33.30868280113764	30.541428009981143	20.968649639195277
14	15.104148498526781	24.131010602054367	40.43629116464463	20.328549734774228
15	17.201652980970255	17.80442825881198	43.50167786405464	21.49224089616312
16	16.817127933936575	21.977228489503755	27.2736046287188	33.93203894784087
17	19.806586343140058	23.932759859631496	30.692470657727362	25.568183139501084
18	23.416726548855728	27.93184360400083	26.701991344407467	21.949438502735976
19	27.46197190304434	22.358148977584293	24.968547316231447	25.21133180313992
20	20.651797279604477	29.85063172802556	26.956170888651688	22.541400103718274
21	20.68586617551644	23.737881123971246	26.622691047438323	28.95356165307399
22	20.490522335475138	27.672896739850838	23.75450860559381	28.082072319080208
23	20.132159409875563	21.856185074335308	35.57862473285567	22.43303078293346
24	21.362941878231023	21.762699093744114	30.678866354581626	26.195492673443237
25	17.661524937734153	29.774354609533244	27.527202792486705	25.0369176602459
26	17.969772866275513	26.943961898649103	26.986984053896297	28.099281181179087
27	22.00466964798575	24.761459590568613	32.29766215282865	20.936208608616987
28	19.173463004434772	23.147431112227363	30.347363206987726	27.33174267635014
29	20.081579308436297	24.679833771694213	26.777454530232948	28.461132389636546
30	30.12864787179863	23.17429089023304	21.78979142394032	24.907269814028012
31	27.303371309106044	23.811251340082	23.773229056931104	25.112148293880853
32	24.88110769259391	24.54727902309476	22.469890305131727	28.1017229791796
33	21.616191213713137	23.050340572683027	28.001492985063177	27.331975228540667
34	20.185181309315343	22.195478720311808	28.91309757192258	28.70624239845027
35	26.880126322349895	24.47751336593715	23.976479671450264	24.665880640262692
36	23.69730076672457	25.15051940531754	26.337349509663703	24.814830318294185
37	22.2257105050801	26.663503956875523	26.386766850150345	24.72401868789403
38	20.213436400464175	26.766757129468782	25.310980416780037	27.708826053287012
39	26.174446700200694	21.51514728692987	26.79175648995026	25.51864952291918
40	18.014306610761118	20.236110239040396	31.197341463357915	30.55224168684057
41	21.073181848836427	24.262402589701193	24.28833215894477	30.37608340251761
42	22.17001425544928	21.204225008197465	28.05091032554981	28.574850410803442
43	24.018571617935354	24.105778689382365	27.732197548434804	24.143452144247473
44	20.03146431137808	26.069914490559544	25.465046243003087	28.43357495505929
45	21.987460785886874	24.97564015804247	25.193309008374204	27.843590047696452
46	23.332426379790284	21.472822788254252	27.952075644576535	27.24267518737893
47	21.43793995967545	19.540779189369577	33.152524005199865	25.868756845755108
48	20.082974621579446	19.13974293680859	29.564011153203058	31.2132712884089
49	20.367037122306172	22.7905797758662	30.984672485122474	25.857710616705155
50	19.572987667757335	23.81485589903514	29.161114483117874	27.45104195008965
51	20.599705588926795	19.407061679817495	29.37308580478174	30.620146926473975
52	21.80723283822972	19.16381208852797	33.297404019897165	25.731551053345147
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	155.0
1	248.0
2	341.0
3	1196.5
4	2052.0
5	1444.5
6	837.0
7	882.5
8	928.0
9	976.0
10	1024.0
11	1042.0
12	1060.0
13	998.0
14	930.0
15	924.0
16	904.5
17	885.0
18	952.5
19	1020.0
20	1045.5
21	1071.0
22	1187.5
23	1304.0
24	1679.5
25	2055.0
26	2380.0
27	2705.0
28	3938.5
29	5172.0
30	5732.0
31	6292.0
32	7391.5
33	8491.0
34	10025.5
35	11560.0
36	12918.5
37	14277.0
38	15499.5
39	18932.0
40	21142.0
41	28453.0
42	35764.0
43	45216.0
44	54668.0
45	60191.5
46	65715.0
47	68151.0
48	70587.0
49	82319.0
50	94051.0
51	98591.0
52	103131.0
53	90763.5
54	78396.0
55	67918.5
56	57441.0
57	50276.0
58	43111.0
59	40387.0
60	37663.0
61	36414.0
62	35165.0
63	32733.5
64	24455.0
65	18608.0
66	14964.0
67	11320.0
68	9671.5
69	8023.0
70	6781.0
71	5539.0
72	4852.0
73	4165.0
74	3287.0
75	2409.0
76	1949.5
77	1490.0
78	1239.0
79	988.0
80	602.0
81	216.0
82	209.0
83	202.0
84	145.0
85	88.0
86	49.5
87	11.0
88	9.0
89	6.5
90	6.0
91	3.5
92	1.0
93	1.5
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	860022.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.99030832458475
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.32713115784985	17.325063828935935
2	12.47452559203286	6.234844814956485
3	4.818227217323657	3.6122695121647257
4	2.406767499644387	2.4058344752681258
5	1.293483413847418	1.6162274662391716
6	0.7959580259878899	1.1934741889719123
7	0.5817812999798144	1.0177225844781306
8	0.4471581547206298	0.8939696125056728
9	0.3430984268076661	0.7716721924443195
>10	6.824315146813106	45.631598905176574
>50	0.5424866876596423	8.773756782690647
>100	0.1301408304692302	5.621396455820696
>500	0.010262000968899872	1.7243969345337495
>1k	0.004198091305459039	2.4390780408425727
>5k	4.6645458949544876E-4	0.7386942049712953
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTGCT	6337	0.7368416156795988	No Hit
CTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTGCTT	4962	0.5769619846934148	Illumina Single End Adapter 1 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTGCT	3475	0.40405943103781067	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA	3194	0.3713858482689978	No Hit
TGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTGCTTG	2352	0.27348137605782175	Illumina Single End Adapter 1 (95% over 22bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	1580	0.18371623051503333	No Hit
ACCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTGC	1397	0.162437705081963	No Hit
TCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTGCT	1388	0.1613912202245989	No Hit
CGCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTGC	1386	0.16115866803407355	No Hit
GCCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTGC	1190	0.13836855336258838	No Hit
ACTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTGCT	981	0.11406684945268841	No Hit
AGCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTGC	965	0.11220643192848556	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	947	0.11011346221375731	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.5948685033638674	0.0
2	0.0	0.0	0.0	2.045412791765792	0.0
3	0.0	0.0	0.0	3.085386187795196	0.0
4	0.0	0.0	0.0	3.7509505570787725	0.0
5	0.0	0.0	0.0	7.013541514054292	0.0
6	0.0	0.0	0.0	7.54422561283316	0.0
7	0.0	0.0	0.0	9.463362565143683	0.0
8	0.0	0.0	0.0	11.735862570957487	0.0
9	0.0	0.0	0.0	13.449423386843593	0.0
10	0.0	0.0	0.0	14.651136831383383	0.0
11	0.0	0.0	0.0	15.801223689626545	0.0
12	0.0	0.0	0.0	16.847592270895397	0.0
13	1.1627609526267933E-4	0.0	0.0	17.283395075939918	0.0
14	1.1627609526267933E-4	0.0	0.0	17.597805637530204	0.0
15	1.1627609526267933E-4	0.0	0.0	18.644057942703792	0.0
16	1.1627609526267933E-4	0.0	0.0	19.753913271986065	0.0
17	1.1627609526267933E-4	0.0	0.0	21.090506987030565	0.0
18	1.1627609526267933E-4	0.0	0.0	21.769559383364612	0.0
19	1.1627609526267933E-4	0.0	0.0	22.493959456851105	0.0
20	1.1627609526267933E-4	0.0	0.0	23.38428551827744	0.0
21	1.1627609526267933E-4	0.0	0.0	24.165777154537906	0.0
22	1.1627609526267933E-4	0.0	0.0	25.028313229196463	0.0
23	1.1627609526267933E-4	0.0	0.0	25.668413133617513	0.0
24	1.1627609526267933E-4	0.0	0.0	26.21723630325736	0.0
25	1.1627609526267933E-4	0.0	0.0	26.723967526412114	0.0
26	1.1627609526267933E-4	0.0	0.0	27.19465316003544	0.0
27	1.1627609526267933E-4	0.0	0.0	27.700337898332833	0.0
28	1.1627609526267933E-4	0.0	0.0	28.189976535483975	0.0
29	1.1627609526267933E-4	0.0	0.0	28.792868089420967	0.0
30	1.1627609526267933E-4	0.0	0.0	29.38448086211748	0.0
31	1.1627609526267933E-4	0.0	0.0	29.90214203822693	0.0
32	1.1627609526267933E-4	0.0	0.0	30.39992000204646	0.0
33	2.3255219052535867E-4	0.0	0.0	30.87386136633714	0.0
34	2.3255219052535867E-4	0.0	0.0	31.503728974375075	0.0
35	2.3255219052535867E-4	0.0	0.0	31.98708870238203	0.0
36	2.3255219052535867E-4	0.0	0.0	32.489517710012066	0.0
37	2.3255219052535867E-4	0.0	0.0	33.0028766705968	0.0
38	2.3255219052535867E-4	0.0	0.0	33.50588705870315	0.0
39	2.3255219052535867E-4	0.0	0.0	34.0652913530119	0.0
40	2.3255219052535867E-4	0.0	0.0	34.618998118652776	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTCGTA	20	6.3105643E-4	46.0	26
CGAAACG	20	6.3105643E-4	46.0	46
CGACGGT	50	1.6370905E-11	46.0	27
GCGGTAT	25	3.416434E-5	46.0	13
CCGATAG	30	1.8608534E-6	46.0	11
AATGCGG	120	0.0	46.0	1
CCCGTAT	20	6.3105643E-4	46.0	39
CCTTTAG	20	6.3105643E-4	46.0	44
CTTGAAC	20	6.3105643E-4	46.0	10
AATTCGG	20	6.3105643E-4	46.0	12
GCGATAT	30	1.8608534E-6	46.0	8
CCGGTAT	20	6.3105643E-4	46.0	42
ACGTAGG	55	1.8189894E-12	46.0	1
TGTACGC	20	6.3105643E-4	46.0	13
CGATAGT	20	6.3105643E-4	46.0	9
GTACGTA	20	6.3105643E-4	46.0	46
ATGGGCG	95	0.0	45.999996	4
ACTAAGG	110	0.0	43.909092	1
TCGAAGG	100	0.0	43.699997	1
CTTAAGG	85	0.0	43.294117	1
>>END_MODULE