FastQCFastQC Report
Thu 26 May 2016
SRR1544780_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544780_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1377989
Sequences flagged as poor quality0
Sequence length52
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCCAGCGTAGTCGTATGCCGTCTTCTGCT46700.33889965739929706No Hit
CTGTCTCTTATACACATCTGACGCCAGCGTAGTCGTATGCCGTCTTCTGCTT38390.27859438645736645Illumina Single End Adapter 2 (95% over 21bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC34180.24804261862757976No Hit
GCTGTCTCTTATACACATCTGACGCCAGCGTAGTCGTATGCCGTCTTCTGCT33400.24238219608429387No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27770.20152555644493533No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA14120.10246816193743202No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC13840.10043621538343193No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC13800.10014593730428907No Hit
GGACAGGCAGAATTTGAAACCTAAAGAACTCCCAGATTTCAGGCTTATCCTG13800.10014593730428907No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGAC206.3118944E-446.08
TCTTACG206.3118944E-446.038
CCGTAGG600.046.01
TTCGCGA206.3118944E-446.01
TCACGTC453.110472E-1046.038
CTAACGG1250.045.9999961
CTACGAA1350.044.296311
TACGCGG1500.042.9333341
CGTAGGG5350.042.5607452
GACTAAT4100.040.951228
ATACCGG451.5888872E-840.888891
CGTTTTT21800.040.8302761
CATACGA6100.040.7213117
TAGGGTA2550.040.5882384
CTCACGA850.040.58823423
TCGTCAA402.911147E-740.2546
CTTATGA11900.040.2016825
ACCCGCA11100.040.19819631
CGAATAT1550.040.06451414
CGGGATA4150.039.9036145