FastQCFastQC Report
Thu 26 May 2016
SRR1544779_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544779_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1009280
Sequences flagged as poor quality0
Sequence length52
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCCAGCGTAGTCGTATGCCGTCTTCTGCT63070.624900919467343No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA52300.5181911857958148No Hit
CTGTCTCTTATACACATCTGACGCCAGCGTAGTCGTATGCCGTCTTCTGCTT47520.4708306911857958Illumina Single End Adapter 2 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCCAGCGTAGTCGTATGCCGTCTTCTGCT38850.38492786937222573No Hit
TGTCTCTTATACACATCTGACGCCAGCGTAGTCGTATGCCGTCTTCTGCTTG24990.24760225110970197Illumina Single End Adapter 2 (95% over 22bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC22870.22659717818642994No Hit
ACCTGTCTCTTATACACATCTGACGCCAGCGTAGTCGTATGCCGTCTTCTGC13800.13673113506658213No Hit
TCTGTCTCTTATACACATCTGACGCCAGCGTAGTCGTATGCCGTCTTCTGCT13780.13653297400126824No Hit
CGCTGTCTCTTATACACATCTGACGCCAGCGTAGTCGTATGCCGTCTTCTGC13580.13455136334812937No Hit
GCCTGTCTCTTATACACATCTGACGCCAGCGTAGTCGTATGCCGTCTTCTGC13290.131678027901078No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11530.11423985415345593No Hit
AGCTGTCTCTTATACACATCTGACGCCAGCGTAGTCGTATGCCGTCTTCTGC10520.10423272035510463No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGCAA351.01939804E-746.00000422
CGAATCG206.311086E-446.00000436
ACGTAGG750.046.0000041
CGCCGAT206.311086E-446.00000442
CCGTTGA206.311086E-446.00000432
CTAACGG950.046.01
CTACGTC253.416858E-546.011
TCGGCGT8400.043.8095254
GTCGGCG8650.042.809253
CGAAGGG5600.042.7142872
CGTTTTT11900.042.3277321
AAGACGG1200.042.1666681
CGTAGGG2950.042.1016962
CTCGAGG1650.041.818181
GTCCCCC8750.041.7942859
CTTCTTA8600.041.45348718
TTGTAGG1500.041.41
CCATAGG1000.041.3999981
TACGCGG1450.041.2413831
AACGGGA2400.041.2083323
Produced by FastQC (version 0.11.5)