FastQCFastQC Report
Thu 26 May 2016
SRR1544778_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544778_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1405691
Sequences flagged as poor quality0
Sequence length52
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTGCT81500.5797860269433325TruSeq Adapter, Index 23 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTGCTT64980.46226375497886807TruSeq Adapter, Index 22 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTGCT50170.3569063186717422TruSeq Adapter, Index 23 (95% over 24bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30000.21341816942699354No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC24950.17749277757344964No Hit
TCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTGCT24510.17436264442185376TruSeq Adapter, Index 23 (95% over 24bp)
CGCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTGC22380.1592099543925372TruSeq Adapter, Index 22 (95% over 21bp)
ACCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTGC20920.1488236034804235TruSeq Adapter, Index 22 (95% over 21bp)
GCCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTGC18800.13374205284091598TruSeq Adapter, Index 23 (95% over 23bp)
AGCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTGC16940.12051012633644237TruSeq Adapter, Index 22 (95% over 21bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA16620.11823366586255443No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC15590.11090630871222766No Hit
ACTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTGCT15280.10870098762814871TruSeq Adapter, Index 22 (95% over 22bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCG14590.10379236973132785No Hit
CGTTCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCT14570.10365009095170986No Hit
GCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAATAATT14510.10322325461285589No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAACTCG206.311937E-446.027
GCGAATC206.311937E-446.021
GCGAATA301.8617011E-646.026
CTATCGG551.8189894E-1246.01
ACATTCG206.311937E-446.016
TAAACGG206.311937E-446.01
CCTACGG253.417547E-546.01
TAGTTCG206.311937E-446.036
CGTTAGT206.311937E-446.021
CTAACGG1200.046.01
TACGCGC405.6134013E-946.034
CTTATCG301.8617011E-646.012
CACTAGT206.311937E-446.011
CGTAGCT405.6134013E-946.038
CGAATAC301.8617011E-646.027
GTTCACG206.311937E-446.08
CACACGG800.046.01
TCATACG405.6134013E-946.031
ATACGCA253.417547E-546.013
TAAGCGT206.311937E-446.034
Produced by FastQC (version 0.11.5)