FastQCFastQC Report
Thu 26 May 2016
SRR1544777_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544777_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences933644
Sequences flagged as poor quality0
Sequence length52
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTGCT88890.9520759518617374TruSeq Adapter, Index 23 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTGCTT69840.7480367249187057TruSeq Adapter, Index 22 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTGCT48820.5228973784440322TruSeq Adapter, Index 23 (95% over 24bp)
TGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTGCTTG35040.37530364892828527TruSeq Adapter, Index 23 (96% over 26bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA31480.3371734836832883No Hit
TCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTGCT19950.21367887546002545TruSeq Adapter, Index 23 (95% over 24bp)
CGCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTGC18750.2008260107707006TruSeq Adapter, Index 22 (95% over 21bp)
GCCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTGC17090.18304621461713458TruSeq Adapter, Index 23 (95% over 23bp)
ACCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTGC16960.18165382094245772TruSeq Adapter, Index 22 (95% over 21bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC16180.17329945889439657No Hit
AGCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTGC13620.14588001422383692TruSeq Adapter, Index 22 (95% over 21bp)
ACTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTGCT12530.1342053287977002TruSeq Adapter, Index 22 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12520.1340982215919558No Hit
CTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTGCTTGAAA11360.12167378572560848TruSeq Adapter, Index 22 (95% over 24bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCG11350.1215666785198641No Hit
CGTTCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCT11080.11867478396476601No Hit
GGCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTGC9350.10014523737098936TruSeq Adapter, Index 23 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATCGG551.8189894E-1246.0000041
CTAACGG1500.046.0000041
AACGTAG206.310842E-446.010
CGACGTA206.310842E-446.032
CATGCGG2100.046.01
GACGTAG206.310842E-446.033
TAAACGG253.4166595E-546.01
CAACCGG501.6370905E-1146.01
CCTACGG206.310842E-446.01
CACGACA206.310842E-446.034
GCCGATG253.4166595E-546.08
TACGCGC253.4166595E-546.034
GAACGTA206.310842E-446.09
TCATACG253.4166595E-546.031
TACACCG206.310842E-446.045
TCGCGCC206.310842E-446.040
TCGCGAT206.310842E-446.013
ACGACAC206.310842E-446.035
AATCGTG206.310842E-446.026
TCTCGTC351.0192889E-745.99999646
Produced by FastQC (version 0.11.5)