##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544777_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	933644
Sequences flagged as poor quality	0
Sequence length	52
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.88760705365214	34.0	33.0	34.0	31.0	34.0
2	33.232120594145094	34.0	33.0	34.0	31.0	34.0
3	33.31914841202857	34.0	34.0	34.0	31.0	34.0
4	36.56125782418138	37.0	37.0	37.0	35.0	37.0
5	36.5624124398593	37.0	37.0	37.0	35.0	37.0
6	36.491935898479504	37.0	37.0	37.0	35.0	37.0
7	36.367916464948095	37.0	37.0	37.0	35.0	37.0
8	36.410598686437226	37.0	37.0	37.0	35.0	37.0
9	38.267025761425124	39.0	39.0	39.0	37.0	39.0
10	38.138608506025854	39.0	39.0	39.0	35.0	39.0
11	37.544056406938836	39.0	35.0	39.0	35.0	39.0
12	37.475615973540236	39.0	35.0	39.0	35.0	39.0
13	37.470219912514835	39.0	35.0	39.0	35.0	39.0
14	38.75395546910814	41.0	35.0	41.0	35.0	41.0
15	38.82149941519466	41.0	35.0	41.0	35.0	41.0
16	38.82291215923843	41.0	36.0	41.0	35.0	41.0
17	38.89141257267224	41.0	36.0	41.0	35.0	41.0
18	38.88863099853906	41.0	36.0	41.0	35.0	41.0
19	38.81798629884624	41.0	36.0	41.0	35.0	41.0
20	38.746867114231975	41.0	35.0	41.0	35.0	41.0
21	38.68792494783879	41.0	35.0	41.0	35.0	41.0
22	38.67277249144213	41.0	35.0	41.0	35.0	41.0
23	38.651362832085894	41.0	35.0	41.0	35.0	41.0
24	38.58197128670028	41.0	35.0	41.0	35.0	41.0
25	38.52875292938208	40.0	35.0	41.0	35.0	41.0
26	38.4610997339457	40.0	35.0	41.0	35.0	41.0
27	38.436489711281816	40.0	35.0	41.0	35.0	41.0
28	38.44039591107531	40.0	36.0	41.0	35.0	41.0
29	38.480728200470416	40.0	36.0	41.0	35.0	41.0
30	38.44914442764051	40.0	35.0	41.0	35.0	41.0
31	38.36299917313237	40.0	35.0	41.0	35.0	41.0
32	38.36982939964269	40.0	35.0	41.0	35.0	41.0
33	38.2900805874616	40.0	35.0	41.0	35.0	41.0
34	38.220103165660575	40.0	35.0	41.0	35.0	41.0
35	38.17049432117595	40.0	35.0	41.0	35.0	41.0
36	38.16623788081967	40.0	35.0	41.0	35.0	41.0
37	38.11462506051557	40.0	35.0	41.0	35.0	41.0
38	38.05667363577552	40.0	35.0	41.0	35.0	41.0
39	38.01686188740034	40.0	35.0	41.0	35.0	41.0
40	37.97633144967461	40.0	35.0	41.0	35.0	41.0
41	37.9382066397899	40.0	35.0	41.0	35.0	41.0
42	37.87034030101409	40.0	35.0	41.0	35.0	41.0
43	37.78076118948979	40.0	35.0	41.0	35.0	41.0
44	37.66888128665744	40.0	35.0	41.0	35.0	41.0
45	37.65413690871467	40.0	35.0	41.0	35.0	41.0
46	37.60177433797036	39.0	35.0	41.0	35.0	41.0
47	37.53281871891213	39.0	35.0	41.0	35.0	41.0
48	37.48374755260035	39.0	35.0	41.0	35.0	41.0
49	37.45272395045649	39.0	35.0	41.0	35.0	41.0
50	37.37132461623488	39.0	35.0	41.0	35.0	41.0
51	37.29220666549563	39.0	35.0	41.0	35.0	41.0
52	36.89083098054505	38.0	35.0	40.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	3.0
13	4.0
14	9.0
15	17.0
16	13.0
17	32.0
18	58.0
19	114.0
20	234.0
21	328.0
22	539.0
23	853.0
24	1472.0
25	2273.0
26	3353.0
27	4043.0
28	4175.0
29	4547.0
30	5201.0
31	6669.0
32	8863.0
33	13322.0
34	41818.0
35	176152.0
36	33038.0
37	52016.0
38	96316.0
39	475205.0
40	2976.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.87489021511411	21.21108259679278	23.87130426586579	23.042722922227316
2	26.92203880708279	23.802113010954926	26.397749034964075	22.878099146998213
3	27.693746224471	23.758734592628453	26.29010629319098	22.257412889709567
4	26.386288563949428	25.835221990394626	24.040640758147642	23.7378486875083
5	24.65275843897674	33.7568709272485	21.13000244204429	20.460368191730467
6	82.8925157768914	3.0615523689971766	9.74643440112077	4.299497452990647
7	84.56167447121172	2.7395881085295897	7.183573182069397	5.515164238189288
8	72.9813504933358	5.2132290251959	13.323065322542641	8.482355158925673
9	49.00326034334286	20.4911079597791	15.504624889144042	15.001006807733997
10	39.514097450420074	20.26768232859634	26.569120564155075	13.649099656828511
11	38.30025148771909	17.66840465959188	24.30337473383859	19.72796911885044
12	20.642985977524624	30.879757166543136	29.088817579291465	19.388439276640774
13	14.899469176688331	33.45161539087704	30.22693874753118	21.421976684903456
14	13.885485259906346	25.686235867204203	39.92463937003826	20.503639502851193
15	16.031378126994873	18.493879894263767	44.28550925192043	21.18923272682093
16	16.180471357391042	23.012090261384426	27.741408931027244	33.06602945019729
17	18.788638924472284	24.454931429966884	30.77597028417684	25.980459361383996
18	23.165467780010367	27.90464031258167	27.57357193962581	21.356319967782152
19	26.425275586840378	22.694196074735125	25.441710116489798	25.4388182219347
20	20.650590589132474	29.745277643298728	27.669754210384255	21.934377557184536
21	20.315345035152585	24.24360891303323	26.470581934870253	28.970464116943933
22	18.764325588768312	28.549211476751307	24.15117539447584	28.53528754000454
23	19.17743808132436	21.917990154705645	36.674256997313755	22.230314766656242
24	20.992905218691494	21.377205872902305	31.34899383490924	26.280895073496964
25	17.465757826323525	29.80922064512812	27.33118833302629	25.393833195522063
26	17.635201425811122	27.43197621363175	27.67007553200149	27.26274682855564
27	22.027346611770653	24.220259542180962	33.07770413562343	20.67468971042496
28	17.401921931699878	23.637917664548798	31.39023010912082	27.569930294630502
29	19.853498763982845	23.82203495122338	28.96832197282904	27.35614431196473
30	27.582033408879614	23.14790219826829	23.88865563319638	25.381408759655717
31	24.525943507375402	23.999511591141808	27.188200213357554	24.28634468812524
32	23.368650149307445	24.869650530609096	26.81000466987417	24.951694650209287
33	17.967876406853147	23.416955499098158	27.629589008230116	30.985579085818575
34	19.13930791607936	20.679937963506433	34.360205817206555	25.820548303207648
35	24.11807926790083	24.106297475268946	27.049067953095612	24.726555303734614
36	20.410134912236355	24.45043292732562	28.1429538453629	26.996478315075123
37	20.026584008465754	26.010128057375187	30.66682804152332	23.29645989263574
38	18.475029026052756	25.91962246852119	27.4803886706282	28.124959834797846
39	24.47442494141236	21.528548354619108	27.79646203477985	26.200564669188687
40	16.513788981667528	19.502187129141298	32.936322624040855	31.047701265150312
41	20.264469112424006	24.12311330657081	24.894070973518815	30.718346607486364
42	21.64443835123452	20.639558546940805	29.750954325203182	27.965048776621497
43	22.580876651057576	23.57622391404004	28.792451940996784	25.050447493905597
44	18.21400876565372	25.679488113242304	28.2076465976325	27.898856523471473
45	20.420845632810796	23.78818907420816	27.35068184447177	28.44028344850928
46	22.34095651019018	20.86052071239145	29.867486965053057	26.931035812365312
47	19.69979992373967	19.24823594432139	34.974144320533306	26.077819811405632
48	18.71784106147525	18.35024913136056	30.23122303576095	32.700686771403234
49	19.21781749788999	21.510875665671282	33.501848670371146	25.769458166067583
50	18.48102702957444	22.71518908706102	30.33179670195492	28.47198718140962
51	19.65770679188213	19.42624812026854	30.16203178085009	30.75401330699924
52	20.82656772817048	18.03042701500786	35.046227469999266	26.096777786822383
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	149.0
1	238.0
2	327.0
3	1452.0
4	2577.0
5	1834.5
6	1092.0
7	1185.0
8	1278.0
9	1296.0
10	1314.0
11	1287.0
12	1260.0
13	1226.5
14	1242.5
15	1292.0
16	1221.0
17	1150.0
18	1173.0
19	1196.0
20	1267.5
21	1339.0
22	1470.5
23	1602.0
24	1993.5
25	2385.0
26	3151.5
27	3918.0
28	5155.0
29	6392.0
30	6812.0
31	7232.0
32	7977.0
33	8722.0
34	10779.0
35	12836.0
36	13925.5
37	15015.0
38	16203.5
39	20772.0
40	24152.0
41	32551.0
42	40950.0
43	55340.5
44	69731.0
45	78775.0
46	87819.0
47	103028.0
48	118237.0
49	120522.0
50	122807.0
51	107226.5
52	91646.0
53	77461.5
54	63277.0
55	54504.0
56	45731.0
57	41345.0
58	36959.0
59	35595.5
60	34232.0
61	34039.5
62	33847.0
63	31483.5
64	23117.0
65	17114.0
66	13627.5
67	10141.0
68	8165.5
69	6190.0
70	5253.0
71	4316.0
72	3851.0
73	3386.0
74	2694.5
75	2003.0
76	1655.0
77	1307.0
78	969.5
79	632.0
80	440.0
81	248.0
82	164.5
83	81.0
84	49.0
85	17.0
86	27.0
87	37.0
88	19.0
89	1.5
90	2.0
91	1.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	933644.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.46291900349951
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.66658263124174	17.229887127829155
2	13.291043763180232	6.768575416276512
3	5.4245067998157666	4.143713318329236
4	2.6792929637582086	2.728904788912857
5	1.5157682390960485	1.929794195008988
6	0.9766195332138197	1.4920550442875395
7	0.6336818310663064	1.1294772396901436
8	0.4753200724438675	0.9682429204300574
9	0.35957715642295873	0.8240295608555825
>10	6.3392489667154	42.196481998513455
>50	0.48066589827519496	8.120787912884431
>100	0.14082667474151878	6.0865111910676335
>500	0.010119282017354642	1.7063961206312876
>1k	0.005902914510123541	2.9710016261626793
>5k	8.432735014462202E-4	1.7041415391204497
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTGCT	8889	0.9520759518617374	TruSeq Adapter, Index 23 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTGCTT	6984	0.7480367249187057	TruSeq Adapter, Index 22 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTGCT	4882	0.5228973784440322	TruSeq Adapter, Index 23 (95% over 24bp)
TGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTGCTTG	3504	0.37530364892828527	TruSeq Adapter, Index 23 (96% over 26bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA	3148	0.3371734836832883	No Hit
TCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTGCT	1995	0.21367887546002545	TruSeq Adapter, Index 23 (95% over 24bp)
CGCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTGC	1875	0.2008260107707006	TruSeq Adapter, Index 22 (95% over 21bp)
GCCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTGC	1709	0.18304621461713458	TruSeq Adapter, Index 23 (95% over 23bp)
ACCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTGC	1696	0.18165382094245772	TruSeq Adapter, Index 22 (95% over 21bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	1618	0.17329945889439657	No Hit
AGCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTGC	1362	0.14588001422383692	TruSeq Adapter, Index 22 (95% over 21bp)
ACTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTGCT	1253	0.1342053287977002	TruSeq Adapter, Index 22 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1252	0.1340982215919558	No Hit
CTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTGCTTGAAA	1136	0.12167378572560848	TruSeq Adapter, Index 22 (95% over 24bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCG	1135	0.1215666785198641	No Hit
CGTTCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCT	1108	0.11867478396476601	No Hit
GGCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTGC	935	0.10014523737098936	TruSeq Adapter, Index 23 (95% over 23bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.7620677688712186	0.0
2	0.0	0.0	0.0	2.619092502067169	0.0
3	0.0	0.0	0.0	3.9083419376121946	0.0
4	0.0	0.0	0.0	4.693759077335687	0.0
5	0.0	0.0	0.0	8.455899679106812	0.0
6	0.0	0.0	0.0	9.04798831246171	0.0
7	0.0	0.0	0.0	11.185955246325152	0.0
8	0.0	0.0	0.0	13.694727326475617	0.0
9	0.0	0.0	0.0	15.406943117505174	0.0
10	0.0	0.0	0.0	16.80030075703373	0.0
11	0.0	0.0	0.0	18.09672637536363	0.0
12	0.0	0.0	0.0	19.33927706920411	0.0
13	0.0	0.0	0.0	19.852534799131146	0.0
14	0.0	0.0	0.0	20.21530690498734	0.0
15	0.0	0.0	0.0	21.36853018923701	0.0
16	0.0	0.0	0.0	22.565881642253363	0.0
17	1.0710720574437366E-4	0.0	0.0	24.04321133108551	0.0
18	1.0710720574437366E-4	0.0	0.0	24.79606788026271	0.0
19	1.0710720574437366E-4	0.0	0.0	25.580735269545993	0.0
20	2.1421441148874732E-4	0.0	0.0	26.63467017407063	0.0
21	2.1421441148874732E-4	0.0	0.0	27.557291644352667	0.0
22	6.426432344662419E-4	0.0	0.0	28.60040872109712	0.0
23	6.426432344662419E-4	0.0	0.0	29.320811786933778	0.0
24	6.426432344662419E-4	0.0	0.0	29.962383949342577	0.0
25	6.426432344662419E-4	0.0	0.0	30.557364477252573	0.0
26	6.426432344662419E-4	0.0	0.0	31.103611226548878	0.0
27	6.426432344662419E-4	0.0	0.0	31.66174687568281	0.0
28	6.426432344662419E-4	0.0	0.0	32.19374836661511	0.0
29	6.426432344662419E-4	0.0	0.0	32.88073398425953	0.0
30	6.426432344662419E-4	0.0	0.0	33.597066976277894	0.0
31	6.426432344662419E-4	0.0	0.0	34.19611757800618	0.0
32	6.426432344662419E-4	0.0	0.0	34.72993989143614	0.0
33	6.426432344662419E-4	0.0	0.0	35.27586531911521	0.0
34	6.426432344662419E-4	0.0	0.0	35.997660778626546	0.0
35	6.426432344662419E-4	0.0	0.0	36.54390752792285	0.0
36	6.426432344662419E-4	0.0	0.0	37.12903419290436	0.0
37	7.497504402106157E-4	0.0	0.0	37.64786149753011	0.0
38	7.497504402106157E-4	0.0	0.0	38.19764278461598	0.0
39	7.497504402106157E-4	0.0	0.0	38.76777444079328	0.0
40	7.497504402106157E-4	0.0	0.0	39.354614821066704	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATCGG	55	1.8189894E-12	46.000004	1
CTAACGG	150	0.0	46.000004	1
AACGTAG	20	6.310842E-4	46.0	10
CGACGTA	20	6.310842E-4	46.0	32
CATGCGG	210	0.0	46.0	1
GACGTAG	20	6.310842E-4	46.0	33
TAAACGG	25	3.4166595E-5	46.0	1
CAACCGG	50	1.6370905E-11	46.0	1
CCTACGG	20	6.310842E-4	46.0	1
CACGACA	20	6.310842E-4	46.0	34
GCCGATG	25	3.4166595E-5	46.0	8
TACGCGC	25	3.4166595E-5	46.0	34
GAACGTA	20	6.310842E-4	46.0	9
TCATACG	25	3.4166595E-5	46.0	31
TACACCG	20	6.310842E-4	46.0	45
TCGCGCC	20	6.310842E-4	46.0	40
TCGCGAT	20	6.310842E-4	46.0	13
ACGACAC	20	6.310842E-4	46.0	35
AATCGTG	20	6.310842E-4	46.0	26
TCTCGTC	35	1.0192889E-7	45.999996	46
>>END_MODULE