FastQCFastQC Report
Thu 26 May 2016
SRR1544775_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544775_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences888553
Sequences flagged as poor quality0
Sequence length52
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTCTTCTGCT53900.6066042205698478No Hit
CTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTCTTCTGCTT48390.5445932881887743Illumina Single End Adapter 1 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTCTTCTGCT32860.36981474374629314No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA27950.3145563629856632No Hit
TGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTCTTCTGCTTG22880.25749730179291497Illumina Single End Adapter 1 (95% over 22bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA20290.22834878729800023No Hit
TCTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTCTTCTGCT12230.13763951053004153No Hit
ACCTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTCTTCTGC11850.1333628945037606No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC11000.12379677970813222No Hit
CGCTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTCTTCTGC10970.12345915212711002No Hit
GCCTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTCTTCTGC10970.12345915212711002No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10040.11299269711542248No Hit
AGCTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTCTTCTGC9760.1098415063592155No Hit
ACTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTCTTCTGCT9280.10443946506286063No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCATA206.3106767E-446.00000439
CAAACGT206.3106767E-446.00000445
CCCTATA206.3106767E-446.00000418
CGTTGAC206.3106767E-446.00000417
TTATCGG405.6097633E-946.0000041
TGACACG206.3106767E-446.00000410
CGAATGC206.3106767E-446.00000442
GCGATCG206.3106767E-446.0000048
TCATCGC206.3106767E-446.00000427
ATGCGAT206.3106767E-446.00000426
TTTGACG206.3106767E-446.00000410
CAATCGG405.6097633E-946.0000041
GTTGGTA206.3106767E-446.00000429
GCCACGA206.3106767E-446.00000445
ATACCGG405.6097633E-946.0000041
GCCAACG206.3106767E-446.00000417
TGCGTTG206.3106767E-446.00000415
GTGCCCG206.3106767E-446.00000430
ACACGCG301.8609226E-646.035
GTCGAGG501.6370905E-1146.01
Produced by FastQC (version 0.11.5)