FastQCFastQC Report
Thu 26 May 2016
SRR1544774_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544774_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1334883
Sequences flagged as poor quality0
Sequence length52
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTGCT66070.4949497446592698No Hit
CTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTGCTT63830.478169247791754Illumina Single End Adapter 1 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTGCT40010.2997266427095109No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC29300.21949489206170128No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28750.21537468077726662No Hit
TCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTGCT19020.14248439750899516No Hit
CGCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTGC18880.14143561645477545No Hit
ACCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTGC17510.1311725447099109No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA16290.12203316695171038No Hit
AGCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTGC14410.10794953565218825No Hit
GCCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTGC14230.10660110286819144No Hit
CGTTCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCT13470.10090772000242718No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGATC206.311822E-446.021
GGCAACG206.311822E-446.023
TAACGGC206.311822E-446.02
TACGGAC206.311822E-446.010
TCGATGG800.046.01
GTCGTTA206.311822E-446.013
TCCACCG301.8616302E-646.030
TATTACG206.311822E-446.013
GTTCACG301.8616302E-646.022
TTATTCG206.311822E-446.032
TGACTCG253.417455E-546.015
AACACGG453.110472E-1046.01
CTAGCGG3750.044.7733351
AGTACGG1300.044.230771
ACGGGAT2150.043.8604664
GCTTAGG2800.043.5357171
ATGGGCG1750.043.371434
ACTACGG850.043.2941171
AATGCGG1500.042.933331
CGTTTTT21250.042.8611761
Produced by FastQC (version 0.11.5)