##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544774_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1334883
Sequences flagged as poor quality	0
Sequence length	52
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.87345632538582	34.0	31.0	34.0	31.0	34.0
2	33.183945709099596	34.0	33.0	34.0	31.0	34.0
3	33.230928103811344	34.0	33.0	34.0	31.0	34.0
4	36.534263302476695	37.0	37.0	37.0	35.0	37.0
5	36.53183387607753	37.0	37.0	37.0	35.0	37.0
6	36.648095750713736	37.0	37.0	37.0	35.0	37.0
7	36.61693496733422	37.0	37.0	37.0	35.0	37.0
8	36.50427565561926	37.0	37.0	37.0	35.0	37.0
9	38.29720507340344	39.0	39.0	39.0	37.0	39.0
10	38.079847447304374	39.0	39.0	39.0	35.0	39.0
11	37.380629613232024	39.0	35.0	39.0	35.0	39.0
12	37.291973903330856	39.0	35.0	39.0	35.0	39.0
13	37.27175190634685	39.0	35.0	39.0	35.0	39.0
14	38.55259299878716	41.0	35.0	41.0	35.0	41.0
15	38.60700525813873	41.0	35.0	41.0	35.0	41.0
16	38.5711886360078	41.0	35.0	41.0	35.0	41.0
17	38.64828977520877	41.0	36.0	41.0	35.0	41.0
18	38.63443313009455	40.0	36.0	41.0	35.0	41.0
19	38.550152335448125	40.0	35.0	41.0	35.0	41.0
20	38.46444894421459	40.0	35.0	41.0	35.0	41.0
21	38.403421123798864	40.0	35.0	41.0	35.0	41.0
22	38.389634147711824	40.0	35.0	41.0	35.0	41.0
23	38.350623987270794	40.0	35.0	41.0	35.0	41.0
24	38.30154852522656	40.0	35.0	41.0	35.0	41.0
25	38.264110787237534	40.0	35.0	41.0	35.0	41.0
26	38.196961081982465	40.0	35.0	41.0	35.0	41.0
27	38.189122192731496	40.0	35.0	41.0	35.0	41.0
28	38.2184925570256	40.0	35.0	41.0	35.0	41.0
29	38.19801660520061	40.0	35.0	41.0	35.0	41.0
30	38.146955950446596	40.0	35.0	41.0	35.0	41.0
31	38.089125413987595	40.0	35.0	41.0	35.0	41.0
32	38.01030052821109	40.0	35.0	41.0	35.0	41.0
33	37.89408884523962	40.0	35.0	41.0	35.0	41.0
34	37.82201061815905	40.0	35.0	41.0	35.0	41.0
35	37.75679291743172	40.0	35.0	41.0	35.0	41.0
36	37.70410365552637	40.0	35.0	41.0	35.0	41.0
37	37.60802557227862	40.0	35.0	41.0	34.0	41.0
38	37.568738233987546	40.0	35.0	41.0	35.0	41.0
39	37.44683316815032	40.0	35.0	41.0	34.0	41.0
40	37.35061949249485	39.0	35.0	41.0	34.0	41.0
41	37.311063965905625	39.0	35.0	41.0	34.0	41.0
42	37.241520792458964	39.0	35.0	41.0	34.0	41.0
43	37.225430992828585	39.0	35.0	41.0	34.0	41.0
44	37.20775603554768	39.0	35.0	41.0	34.0	41.0
45	37.187111529624694	39.0	35.0	41.0	34.0	41.0
46	37.1486969269966	39.0	35.0	41.0	34.0	41.0
47	37.05286081252065	38.0	35.0	41.0	34.0	41.0
48	37.03647360854846	38.0	35.0	41.0	34.0	41.0
49	36.96772975609098	38.0	35.0	41.0	34.0	41.0
50	36.87739150172712	38.0	35.0	41.0	34.0	41.0
51	36.792350340816384	37.0	35.0	41.0	34.0	41.0
52	36.52360469044853	37.0	35.0	40.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	4.0
11	0.0
12	2.0
13	3.0
14	4.0
15	13.0
16	16.0
17	45.0
18	96.0
19	208.0
20	351.0
21	588.0
22	911.0
23	1582.0
24	2716.0
25	4496.0
26	6271.0
27	7387.0
28	7869.0
29	8746.0
30	10430.0
31	12847.0
32	16590.0
33	22986.0
34	71318.0
35	274450.0
36	51249.0
37	77161.0
38	131527.0
39	618515.0
40	6500.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.48906458468645	21.56129039024394	24.88832354595871	21.0613214791109
2	28.26644732160047	24.21006185560832	25.78061148430237	21.74287933848884
3	27.103124393673454	24.778126622333193	25.51159914389501	22.607149840098344
4	26.534610149353917	26.912995371129906	22.91766394507983	23.63473053443635
5	24.556683994027942	35.347292609164995	20.60435259120088	19.491670805606184
6	86.37056580988745	2.996217646040889	7.902789982343021	2.730426561728631
7	86.93053997990835	2.8694649643451897	5.813393383539981	4.386601672206478
8	76.04194524913419	5.851524066154113	11.219784805110262	6.886745879601433
9	50.80422778625543	20.730955447031686	14.754551522492982	13.710265244219904
10	41.983005252145695	20.00220244021386	24.343257049494223	13.67153525814622
11	40.423767476250724	17.12022701615048	24.096643675887698	18.359361831711094
12	21.22455675890696	32.10416193778781	26.848794988025166	19.822486315280067
13	14.521871954321092	34.84200487982842	30.571218601180778	20.064904564669714
14	15.493942165717895	24.83933048814016	40.825750271746664	18.840977074395283
15	16.9671049822344	17.61277954697153	44.977874465402586	20.442241005391484
16	16.178346716528715	21.987994453446483	27.934358292075036	33.89930053794977
17	20.32979669379264	23.419355853659084	30.85783548071254	25.393011971835733
18	23.773993675850242	28.48788994990572	26.823099852196787	20.91501652204725
19	28.60393008226189	22.45957136318314	25.204456120873513	23.732042433681457
20	21.747823592030162	30.22204942305805	26.59409101771466	21.436035967197125
21	20.004599654052075	24.263474776441083	27.18560353229459	28.546322037212253
22	20.7719328210787	28.72581342334871	23.13978078977708	27.362472965795504
23	20.70376205255442	21.96986552379497	35.71286771949302	21.61350470415759
24	19.316599282483935	21.64773991428462	32.4165488660804	26.619111937151047
25	16.953246089732207	30.922110776749722	28.67262524131328	23.452017892204786
26	17.4067689827498	29.552402719938755	27.46652702896059	25.574301268350858
27	19.25539541667697	27.4631559470006	33.74063494703281	19.540813689289624
28	17.555021676057002	24.610846044185145	31.72427845736293	26.109853822394925
29	18.363032565400864	28.8775870244808	29.570756388387597	23.188624021730746
30	26.634244349504787	23.62012251260972	24.504394767181843	25.241238370703652
31	27.144476332382688	26.323805157455748	25.66262361570265	20.869094894458915
32	24.494056782504533	26.47595332325005	25.709219459682984	23.32077043456243
33	18.253135293505125	24.793783425214045	29.967420365679985	26.985660915600846
34	21.33550281185692	23.706796775447735	30.14571314489734	24.811987267798003
35	24.618337337429573	22.88387821254747	28.384585016065078	24.11319943395788
36	24.23291030000382	26.45243066246255	28.330572791772763	20.984086245760864
37	21.692013457359185	26.54929308411299	28.387431707497964	23.371261751029866
38	22.39844241030862	25.13823308859278	26.605402870513746	25.85792163058485
39	27.41663501595271	21.996684353610014	26.97547275678842	23.61120787364885
40	18.188710171603052	21.287034144565478	31.50073826694924	29.02351741688223
41	21.63897510118864	24.639762436108633	24.17739981706262	29.543862645640107
42	22.214006770630835	21.79464417480783	27.939002893886585	28.05234616067476
43	22.35334482497717	25.693188092139913	29.117832798829564	22.83563428405336
44	18.665081509016147	28.779900560573473	27.06124806443711	25.49376986597327
45	20.799126215555972	26.267920109852323	27.295425891257885	25.63752778333382
46	23.65278455115542	21.97128886951141	28.522200072965198	25.853726506367973
47	21.757187708585697	19.372109765425137	33.564814294586114	25.305888231403053
48	20.768861390848485	19.721578595277638	30.162493641764858	29.347066372109015
49	19.971188486181934	23.24877910648349	32.48809071656467	24.291941690769903
50	19.182205481678917	24.849968124547246	29.512024649351293	26.455801744422548
51	20.479322906951396	20.36193434181123	30.558483402665253	28.60025934857212
52	21.82872955906997	18.77685160422299	33.913159430451955	25.48125940625508
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	314.0
1	530.0
2	746.0
3	3120.0
4	5494.0
5	3683.0
6	1872.0
7	2002.0
8	2132.0
9	2193.0
10	2254.0
11	2126.0
12	1998.0
13	1941.5
14	1901.5
15	1918.0
16	1891.0
17	1864.0
18	1886.0
19	1908.0
20	2313.5
21	2719.0
22	3028.5
23	3338.0
24	4050.5
25	4763.0
26	5520.5
27	6278.0
28	8956.5
29	11635.0
30	12735.5
31	13836.0
32	14245.0
33	14654.0
34	17822.5
35	20991.0
36	22839.0
37	24687.0
38	27881.5
39	35132.0
40	39188.0
41	51843.0
42	64498.0
43	84401.0
44	104304.0
45	117174.5
46	130045.0
47	140354.0
48	150663.0
49	151382.0
50	152101.0
51	136127.0
52	120153.0
53	102225.5
54	84298.0
55	73720.5
56	63143.0
57	57096.5
58	51050.0
59	50994.5
60	50939.0
61	51367.5
62	51796.0
63	46740.5
64	34458.0
65	27231.0
66	21559.0
67	15887.0
68	13159.5
69	10432.0
70	8851.5
71	7271.0
72	6460.0
73	5649.0
74	4706.0
75	3763.0
76	3060.0
77	2357.0
78	1828.5
79	1300.0
80	919.5
81	539.0
82	355.5
83	172.0
84	106.5
85	41.0
86	24.0
87	7.0
88	5.5
89	3.5
90	3.0
91	2.5
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1334883.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.58815965911936
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.5108149442427	14.35846092074453
2	13.521522227295527	5.83809561354374
3	5.698684225427924	3.6907231471632893
4	2.9932410470358657	2.584742624865595
5	1.717381680148223	1.8537554953343245
6	1.0701410137421767	1.3861425037462776
7	0.7538792374661976	1.13924057394808
8	0.5158128626022046	0.890836034566703
9	0.37156627168281886	0.7219288797329173
>10	4.699896397955585	27.116592752490188
>50	1.869369178959302	26.595900994252837
>100	0.2613356842038108	9.153167675806422
>500	0.010091524423302448	1.5830723595484215
>1k	0.005567737612856523	2.1114893375327424
>5k	6.959672016070654E-4	0.9758510867239593
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTGCT	6607	0.4949497446592698	No Hit
CTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTGCTT	6383	0.478169247791754	Illumina Single End Adapter 1 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTGCT	4001	0.2997266427095109	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	2930	0.21949489206170128	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2875	0.21537468077726662	No Hit
TCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTGCT	1902	0.14248439750899516	No Hit
CGCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTGC	1888	0.14143561645477545	No Hit
ACCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTGC	1751	0.1311725447099109	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA	1629	0.12203316695171038	No Hit
AGCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTGC	1441	0.10794953565218825	No Hit
GCCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTGC	1423	0.10660110286819144	No Hit
CGTTCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCT	1347	0.10090772000242718	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	7.491293244426665E-5	0.0	0.0	0.48648458329306765	0.0
2	7.491293244426665E-5	0.0	0.0	1.5356402021750222	0.0
3	7.491293244426665E-5	0.0	0.0	2.4203619343418112	0.0
4	7.491293244426665E-5	0.0	0.0	2.9990643374737713	0.0
5	7.491293244426665E-5	0.0	0.0	5.691584955385603	0.0
6	7.491293244426665E-5	0.0	0.0	6.151175795931179	0.0
7	7.491293244426665E-5	0.0	0.0	7.9300582897527345	0.0
8	7.491293244426665E-5	0.0	0.0	10.09009778385072	0.0
9	7.491293244426665E-5	0.0	0.0	11.682297250021163	0.0
10	7.491293244426665E-5	0.0	0.0	12.748233365770632	0.0
11	7.491293244426665E-5	0.0	0.0	13.802258325261464	0.0
12	7.491293244426665E-5	0.0	0.0	14.760020166561414	0.0
13	7.491293244426665E-5	0.0	0.0	15.153088323096481	0.0
14	7.491293244426665E-5	0.0	0.0	15.426145961855832	0.0
15	7.491293244426665E-5	0.0	0.0	16.306372918075965	0.0
16	7.491293244426665E-5	0.0	0.0	17.294849061678065	0.0
17	7.491293244426665E-5	0.0	0.0	18.606125031182508	0.0
18	7.491293244426665E-5	0.0	0.0	19.281015639572907	0.0
19	7.491293244426665E-5	0.0	0.0	20.02535053633914	0.0
20	7.491293244426665E-5	0.0	0.0	20.91658969362858	0.0
21	7.491293244426665E-5	0.0	0.0	21.680626691627655	0.0
22	7.491293244426665E-5	0.0	0.0	22.54669510361582	0.0
23	7.491293244426665E-5	0.0	0.0	23.2061536479227	0.0
24	2.2473879733279995E-4	0.0	0.0	23.760659173875165	0.0
25	2.2473879733279995E-4	0.0	0.0	24.292166429567235	0.0
26	2.2473879733279995E-4	0.0	0.0	24.74928514334215	0.0
27	2.2473879733279995E-4	0.0	0.0	25.249104228610296	0.0
28	2.996517297770666E-4	0.0	0.0	25.722928526320285	0.0
29	2.996517297770666E-4	0.0	0.0	26.368902742787196	0.0
30	2.996517297770666E-4	0.0	0.0	26.99509994508882	0.0
31	2.996517297770666E-4	0.0	0.0	27.566385967908797	0.0
32	2.996517297770666E-4	0.0	0.0	28.08680610959912	0.0
33	2.996517297770666E-4	0.0	0.0	28.588572931110818	0.0
34	2.996517297770666E-4	0.0	0.0	29.254998378135014	0.0
35	2.996517297770666E-4	0.0	0.0	29.760660672133813	0.0
36	2.996517297770666E-4	0.0	0.0	30.306176646192963	0.0
37	2.996517297770666E-4	0.0	0.0	30.845025369264572	0.0
38	2.996517297770666E-4	0.0	0.0	31.349339230479377	0.0
39	2.996517297770666E-4	0.0	0.0	31.984750723471645	0.0
40	2.996517297770666E-4	0.0	0.0	32.55521270403474	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCGATC	20	6.311822E-4	46.0	21
GGCAACG	20	6.311822E-4	46.0	23
TAACGGC	20	6.311822E-4	46.0	2
TACGGAC	20	6.311822E-4	46.0	10
TCGATGG	80	0.0	46.0	1
GTCGTTA	20	6.311822E-4	46.0	13
TCCACCG	30	1.8616302E-6	46.0	30
TATTACG	20	6.311822E-4	46.0	13
GTTCACG	30	1.8616302E-6	46.0	22
TTATTCG	20	6.311822E-4	46.0	32
TGACTCG	25	3.417455E-5	46.0	15
AACACGG	45	3.110472E-10	46.0	1
CTAGCGG	375	0.0	44.773335	1
AGTACGG	130	0.0	44.23077	1
ACGGGAT	215	0.0	43.860466	4
GCTTAGG	280	0.0	43.535717	1
ATGGGCG	175	0.0	43.37143	4
ACTACGG	85	0.0	43.294117	1
AATGCGG	150	0.0	42.93333	1
CGTTTTT	2125	0.0	42.861176	1
>>END_MODULE