FastQCFastQC Report
Thu 26 May 2016
SRR1544773_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544773_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1006277
Sequences flagged as poor quality0
Sequence length52
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTGCT89590.8903115146227133No Hit
CTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTGCTT82490.8197544016210248Illumina Single End Adapter 1 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTGCT49980.4966823250456882No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA42440.421752658562205No Hit
TGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTGCTTG38440.3820021723640707Illumina Single End Adapter 1 (95% over 22bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC19880.1975599164047275No Hit
TCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTGCT19860.19736116397373688No Hit
ACCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTGC19150.19030545267356802No Hit
CGCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTGC17260.17152334794494956No Hit
GCCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTGC16100.1599957069474906No Hit
AGCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTGC15020.14926307567399433No Hit
ACTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTGCT13070.12988471365240387No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12840.12759906069601112No Hit
CGTTCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCT10500.10434502627010257No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGGGT351.01939804E-746.0000043
GTTGACG253.41685E-546.031
ATAGGCG253.41685E-546.09
GGATCGT253.41685E-546.07
ATTGTGC301.86117E-646.026
AGGCGTA206.311079E-446.02
CACGACG453.092282E-1046.025
GTTAACG301.86117E-646.026
CGAGGGT650.046.03
CAAATAG206.311079E-446.025
GCGATCG206.311079E-446.08
ACGGGAT1600.044.56254
GCTACGA1550.044.51613210
CATGCGG2700.044.29631
AACGGGA1300.044.230773
AGTACGG1050.043.8095251
ATCGCGG2050.043.7560961
CTTAAGG900.043.4444471
GTCCCCC7200.043.4444479
ACGGGAC1750.043.371434
Produced by FastQC (version 0.11.5)