##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544773_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1006277
Sequences flagged as poor quality	0
Sequence length	52
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.87481776886484	34.0	33.0	34.0	31.0	34.0
2	33.215869984109744	34.0	33.0	34.0	31.0	34.0
3	33.30480871569161	34.0	34.0	34.0	31.0	34.0
4	36.55336154955345	37.0	37.0	37.0	35.0	37.0
5	36.55971864605869	37.0	37.0	37.0	35.0	37.0
6	36.49748925991551	37.0	37.0	37.0	35.0	37.0
7	36.35277363986258	37.0	37.0	37.0	35.0	37.0
8	36.39873613329133	37.0	37.0	37.0	35.0	37.0
9	38.25195150043179	39.0	39.0	39.0	37.0	39.0
10	38.11789795453936	39.0	39.0	39.0	35.0	39.0
11	37.52714511014363	39.0	35.0	39.0	35.0	39.0
12	37.48916550810562	39.0	35.0	39.0	35.0	39.0
13	37.473411396663145	39.0	35.0	39.0	35.0	39.0
14	38.74880674009244	41.0	35.0	41.0	35.0	41.0
15	38.78968216504998	41.0	35.0	41.0	35.0	41.0
16	38.768524968771025	41.0	35.0	41.0	35.0	41.0
17	38.856598133515924	41.0	36.0	41.0	35.0	41.0
18	38.855495057523925	41.0	36.0	41.0	35.0	41.0
19	38.78428901783505	41.0	36.0	41.0	35.0	41.0
20	38.71791464974356	41.0	35.0	41.0	35.0	41.0
21	38.662918858326286	41.0	35.0	41.0	35.0	41.0
22	38.65237504186223	41.0	35.0	41.0	35.0	41.0
23	38.62949664953089	41.0	35.0	41.0	35.0	41.0
24	38.564331689981984	41.0	35.0	41.0	35.0	41.0
25	38.51712699385954	40.0	35.0	41.0	35.0	41.0
26	38.46187282428198	40.0	35.0	41.0	35.0	41.0
27	38.46303254471681	40.0	35.0	41.0	35.0	41.0
28	38.5207591945359	40.0	36.0	41.0	35.0	41.0
29	38.520155980907845	40.0	36.0	41.0	35.0	41.0
30	38.50677199220493	40.0	35.0	41.0	35.0	41.0
31	38.467386216717664	40.0	35.0	41.0	35.0	41.0
32	38.4142080162818	40.0	35.0	41.0	35.0	41.0
33	38.34059508465363	40.0	35.0	41.0	35.0	41.0
34	38.28178424032349	40.0	35.0	41.0	35.0	41.0
35	38.187032993897304	40.0	35.0	41.0	35.0	41.0
36	38.190312409008655	40.0	35.0	41.0	35.0	41.0
37	38.09973396987112	40.0	35.0	41.0	35.0	41.0
38	38.05898972151803	40.0	35.0	41.0	35.0	41.0
39	38.01581671845824	40.0	35.0	41.0	35.0	41.0
40	37.968651772822	40.0	35.0	41.0	35.0	41.0
41	37.92878601021389	40.0	35.0	41.0	35.0	41.0
42	37.87698019531401	40.0	35.0	41.0	35.0	41.0
43	37.789847129567704	40.0	35.0	41.0	35.0	41.0
44	37.67195613136343	40.0	35.0	41.0	35.0	41.0
45	37.66075146306633	40.0	35.0	41.0	35.0	41.0
46	37.614545497909624	39.0	35.0	41.0	35.0	41.0
47	37.53105556422337	39.0	35.0	41.0	35.0	41.0
48	37.48560585206658	39.0	35.0	41.0	35.0	41.0
49	37.43267311088299	39.0	35.0	41.0	35.0	41.0
50	37.35183055957753	39.0	35.0	41.0	35.0	41.0
51	37.25961936921941	39.0	35.0	41.0	34.0	41.0
52	36.835678446391995	38.0	35.0	40.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	2.0
12	2.0
13	6.0
14	10.0
15	11.0
16	15.0
17	31.0
18	58.0
19	122.0
20	200.0
21	335.0
22	554.0
23	889.0
24	1479.0
25	2434.0
26	3507.0
27	4211.0
28	4473.0
29	4901.0
30	5778.0
31	7420.0
32	9898.0
33	14149.0
34	45169.0
35	192916.0
36	35904.0
37	56706.0
38	100455.0
39	509961.0
40	4680.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.973800454546808	21.061496983434978	23.564187594469516	23.4005149675487
2	27.759056402958628	24.25028098624931	25.988470371478233	22.00219223931383
3	27.478020465537817	24.412761098584188	25.59116426192788	22.518054173950112
4	26.472830045802496	26.205905530982026	23.511418824041492	23.809845599173983
5	24.17038250899106	34.483447400666016	20.835416093183092	20.510753997159828
6	83.72684658399227	3.0019567176831035	9.256596344744041	4.014600353580574
7	84.38819529811374	2.8633268970671097	7.008110092946575	5.740367711872576
8	72.0443774427916	5.795819640118973	13.413503438913937	8.746299478175493
9	48.62388785592834	19.797729650980795	16.178249130209675	15.400133362881194
10	39.404855720641535	19.555351061387668	26.895576466519657	14.144216751451141
11	37.424188369603996	17.13792524324813	25.111872774593873	20.326013612553997
12	20.334858095733082	29.95358136974213	28.82198440389674	20.889576130628047
13	14.215668250392287	32.990816643926074	31.743446387028623	21.050068718653016
14	15.149605923617454	24.75829219986147	39.38816051643831	20.703941360082762
15	16.140684920752438	17.932139957486857	44.21774521329614	21.709429908464568
16	15.436902562614469	23.361559491074523	28.005410041171565	33.19612790513944
17	19.124753919646377	23.82614329851522	31.05715424281783	25.991948539020566
18	22.406156555302367	28.768420623744756	27.521845376571264	21.303577444381617
19	26.762014832893925	22.32556244453565	25.589971747341934	25.322450975228488
20	20.519797232769903	30.87440138252191	27.062429132336323	21.54337225237186
21	19.349940423958813	24.222455645910618	27.154749636531495	29.272854293599078
22	19.78312134730298	28.848219724787512	23.265065185828554	28.103593742080957
23	19.59539967623229	22.060227949163103	35.759139878979646	22.585232495624965
24	19.898397757277568	21.913250526445502	31.202939150949486	26.985412565327437
25	16.651876173260444	30.31709956602407	28.467608819440372	24.563415441275115
26	17.169825008422134	28.66069680614781	27.00280340303912	27.16667478239093
27	20.0788649646171	26.422645056977352	32.86411196916952	20.634378009236027
28	17.556299110483494	24.021119433317068	31.43985204869037	26.982729407509066
29	18.686107304449965	28.338220986865448	29.139988293481817	23.835683415202773
30	25.61501455364676	23.033916108586403	24.48848577479163	26.862583562975207
31	26.942680792664447	25.075799208369066	25.775407765456233	22.206112233510254
32	23.65223492139838	25.470422160101048	26.03895348894986	24.838389429550713
33	18.02465921411301	23.435197266756568	30.724045168477467	27.81609835065295
34	22.372964899326924	21.87936323696159	30.75972122984029	24.98795063387119
35	23.56041129828069	21.56215435710048	29.468824190555882	25.40861015406295
36	25.536904848267426	25.046284472366953	28.246397363747754	21.170413315617868
37	21.814569944458633	25.179249848699715	29.70941400827009	23.296766198571568
38	22.693651946730373	23.737797842939866	26.34433659916703	27.224213611162735
39	28.53369400274477	20.47318978770259	27.25571587147475	23.737400338077887
40	18.855941256731494	19.929999393805087	31.584345066020585	29.62971428344283
41	21.966019296873522	23.83270212873791	23.99637475565873	30.204903818729832
42	21.85958737007802	19.964482940581966	28.90675231571426	29.269177373625748
43	23.57283332521761	23.97292196880183	29.29392205128409	23.16032265469647
44	19.01673197340295	26.320287555017156	28.503980514311667	26.158999957268225
45	20.414756572991333	24.208642351956765	27.985634174287995	27.390966900763903
46	24.336539541299267	19.892534560563345	28.935273289561426	26.835652608575966
47	20.797156250217384	18.09243379308083	34.939882358436094	26.170527598265686
48	20.674526000296144	17.991566934353067	30.117055244231956	31.216851821118833
49	20.31706975315942	21.014690785936676	33.44138840498193	25.22685105592198
50	19.780736318131094	22.195478978452254	30.755448052573993	27.268336650842663
51	20.476667955244928	18.330936710269636	30.92726952916543	30.265125805320007
52	21.25965315713268	17.502238449254033	35.9901895800063	25.24791881360699
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	189.0
1	308.0
2	427.0
3	1587.5
4	2748.0
5	1930.5
6	1113.0
7	1191.0
8	1269.0
9	1290.5
10	1312.0
11	1266.5
12	1221.0
13	1218.0
14	1207.5
15	1200.0
16	1120.5
17	1041.0
18	1156.5
19	1272.0
20	1512.5
21	1753.0
22	1971.0
23	2189.0
24	2568.0
25	2947.0
26	3430.5
27	3914.0
28	5343.5
29	6773.0
30	7607.0
31	8441.0
32	8871.0
33	9301.0
34	11370.5
35	13440.0
36	14650.5
37	15861.0
38	18264.0
39	23522.0
40	26377.0
41	34733.5
42	43090.0
43	57767.0
44	72444.0
45	84060.0
46	95676.0
47	112184.5
48	128693.0
49	130909.0
50	133125.0
51	116032.5
52	98940.0
53	83429.0
54	67918.0
55	58054.5
56	48191.0
57	42656.5
58	37122.0
59	36245.5
60	35369.0
61	35554.0
62	35739.0
63	33997.5
64	25509.0
65	18762.0
66	15291.0
67	11820.0
68	9519.0
69	7218.0
70	6325.0
71	5432.0
72	4749.5
73	4067.0
74	3337.0
75	2607.0
76	2115.5
77	1624.0
78	1313.0
79	1002.0
80	684.5
81	367.0
82	240.5
83	114.0
84	67.5
85	21.0
86	13.0
87	5.0
88	3.5
89	1.5
90	1.0
91	1.0
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1006277.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.727656770183387
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.28358621834063	16.637654262741684
2	13.262076108313384	6.558801321328454
3	5.494756721968262	4.076173747694675
4	2.684569624031843	2.655324649548787
5	1.5447356835509394	1.9098846891751124
6	0.9982863361129299	1.4811169126658676
7	0.708867030516895	1.2270034438424624
8	0.49308989618475796	0.9754366167761643
9	0.3828107387084689	0.8519411299251036
>10	6.438101077036637	41.736960165866634
>50	0.5285145348517561	8.521318532270532
>100	0.16407735349571306	6.978268013933779
>500	0.010481599977613108	1.838122966614166
>1k	0.005240799988806554	2.836584902381154
>5k	8.062769213548545E-4	1.715408645235456
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTGCT	8959	0.8903115146227133	No Hit
CTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTGCTT	8249	0.8197544016210248	Illumina Single End Adapter 1 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTGCT	4998	0.4966823250456882	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA	4244	0.421752658562205	No Hit
TGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTGCTTG	3844	0.3820021723640707	Illumina Single End Adapter 1 (95% over 22bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	1988	0.1975599164047275	No Hit
TCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTGCT	1986	0.19736116397373688	No Hit
ACCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTGC	1915	0.19030545267356802	No Hit
CGCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTGC	1726	0.17152334794494956	No Hit
GCCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTGC	1610	0.1599957069474906	No Hit
AGCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTGC	1502	0.14926307567399433	No Hit
ACTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTGCT	1307	0.12988471365240387	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1284	0.12759906069601112	No Hit
CGTTCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCT	1050	0.10434502627010257	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.8308845377565024	0.0
2	0.0	0.0	0.0	2.56887517055443	0.0
3	0.0	0.0	0.0	3.7946807886893965	0.0
4	0.0	0.0	0.0	4.597143728814233	0.0
5	0.0	0.0	0.0	8.266014228686535	0.0
6	0.0	0.0	0.0	8.88761245660986	0.0
7	0.0	0.0	0.0	11.290231218640592	0.0
8	0.0	0.0	0.0	14.059349463418124	0.0
9	0.0	0.0	0.0	16.165727727057263	0.0
10	0.0	0.0	0.0	17.59545333938866	0.0
11	0.0	0.0	0.0	18.922722073544364	0.0
12	0.0	0.0	0.0	20.18559502005909	0.0
13	0.0	0.0	0.0	20.687146779664047	0.0
14	9.937621549533577E-5	0.0	0.0	21.032479128510342	0.0
15	9.937621549533577E-5	0.0	0.0	22.165864866234646	0.0
16	9.937621549533577E-5	0.0	0.0	23.349634345215083	0.0
17	9.937621549533577E-5	0.0	0.0	24.861742840192115	0.0
18	9.937621549533577E-5	0.0	0.0	25.667882700290278	0.0
19	9.937621549533577E-5	0.0	0.0	26.515263689819005	0.0
20	9.937621549533577E-5	0.0	0.0	27.534168027292683	0.0
21	9.937621549533577E-5	0.0	0.0	28.44445416122996	0.0
22	9.937621549533577E-5	0.0	0.0	29.431955614607112	0.0
23	9.937621549533577E-5	0.0	0.0	30.150942533715867	0.0
24	1.9875243099067155E-4	0.0	0.0	30.760913744426237	0.0
25	1.9875243099067155E-4	0.0	0.0	31.36104670980257	0.0
26	1.9875243099067155E-4	0.0	0.0	31.881479950351643	0.0
27	1.9875243099067155E-4	0.0	0.0	32.43818550955651	0.0
28	1.9875243099067155E-4	0.0	0.0	32.95355056311533	0.0
29	1.9875243099067155E-4	0.0	0.0	33.62702317552721	0.0
30	1.9875243099067155E-4	0.0	0.0	34.28767625614021	0.0
31	1.9875243099067155E-4	0.0	0.0	34.88055475778538	0.0
32	1.9875243099067155E-4	0.0	0.0	35.42374515168289	0.0
33	1.9875243099067155E-4	0.0	0.0	35.93394264203594	0.0
34	1.9875243099067155E-4	0.0	0.0	36.64090503906976	0.0
35	1.9875243099067155E-4	0.0	0.0	37.17038151522891	0.0
36	1.9875243099067155E-4	0.0	0.0	37.72251576852099	0.0
37	1.9875243099067155E-4	0.0	0.0	38.276836298553974	0.0
38	1.9875243099067155E-4	0.0	0.0	38.80601464606664	0.0
39	1.9875243099067155E-4	0.0	0.0	39.45822074836253	0.0
40	1.9875243099067155E-4	0.0	0.0	40.033112155003046	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCGGGT	35	1.01939804E-7	46.000004	3
GTTGACG	25	3.41685E-5	46.0	31
ATAGGCG	25	3.41685E-5	46.0	9
GGATCGT	25	3.41685E-5	46.0	7
ATTGTGC	30	1.86117E-6	46.0	26
AGGCGTA	20	6.311079E-4	46.0	2
CACGACG	45	3.092282E-10	46.0	25
GTTAACG	30	1.86117E-6	46.0	26
CGAGGGT	65	0.0	46.0	3
CAAATAG	20	6.311079E-4	46.0	25
GCGATCG	20	6.311079E-4	46.0	8
ACGGGAT	160	0.0	44.5625	4
GCTACGA	155	0.0	44.516132	10
CATGCGG	270	0.0	44.2963	1
AACGGGA	130	0.0	44.23077	3
AGTACGG	105	0.0	43.809525	1
ATCGCGG	205	0.0	43.756096	1
CTTAAGG	90	0.0	43.444447	1
GTCCCCC	720	0.0	43.444447	9
ACGGGAC	175	0.0	43.37143	4
>>END_MODULE