FastQCFastQC Report
Thu 26 May 2016
SRR1544772_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544772_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences576516
Sequences flagged as poor quality0
Sequence length52
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGCT28550.49521609114057547TruSeq Adapter, Index 13 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGCTT25060.4346800435720778TruSeq Adapter, Index 8 (96% over 25bp)
GCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGCT18700.32436220330398463TruSeq Adapter, Index 13 (95% over 24bp)
ACCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGC9350.16218110165199232TruSeq Adapter, Index 8 (95% over 23bp)
TCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGCT8600.14917192237509452TruSeq Adapter, Index 13 (95% over 24bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7710.13373436296650917No Hit
CGCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGC7140.12384738671606685TruSeq Adapter, Index 8 (95% over 23bp)
GCCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGC6650.11534805625516031TruSeq Adapter, Index 13 (95% over 23bp)
ACTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGCT5800.10060431974134283TruSeq Adapter, Index 8 (95% over 24bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGAGG351.0183794E-746.0000041
ATAGCGG551.8189894E-1246.0000041
TAAACGG351.0183794E-746.0000041
ATAGAGG1400.046.0000041
GTAGGGT351.0183794E-746.0000043
TCAGGGT351.0183794E-746.0000043
GTTACGG351.0183794E-746.0000041
CATAGAC351.0183794E-746.00000416
GGTATGC700.046.0000047
CCGACGG351.0183794E-746.0000041
TTACGGG1450.046.0000042
TCTTTAC551.8189894E-1246.00000410
ACGTAGG351.0183794E-746.0000041
TCATAGA351.0183794E-746.00000415
CGTCAGA351.0183794E-746.00000430
TAGTAGG1150.046.0000041
CTTGTAG206.308824E-446.014
TTCTAGA253.415021E-546.039
TTAGGGT600.046.03
AGCCGTG206.308824E-446.030
Produced by FastQC (version 0.11.5)