##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544772_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	576516
Sequences flagged as poor quality	0
Sequence length	52
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.88071623337427	34.0	31.0	34.0	31.0	34.0
2	33.17884325846984	34.0	33.0	34.0	31.0	34.0
3	33.21611022070506	34.0	33.0	34.0	31.0	34.0
4	36.52039839310618	37.0	37.0	37.0	35.0	37.0
5	36.50618196199238	37.0	37.0	37.0	35.0	37.0
6	36.65638594592345	37.0	37.0	37.0	35.0	37.0
7	36.6353596430975	37.0	37.0	37.0	35.0	37.0
8	36.54990494626342	37.0	37.0	37.0	35.0	37.0
9	38.3325094186458	39.0	39.0	39.0	37.0	39.0
10	38.04318527152759	39.0	39.0	39.0	35.0	39.0
11	36.881104774195336	39.0	35.0	39.0	35.0	39.0
12	36.69230689174282	39.0	35.0	39.0	35.0	39.0
13	36.64764030833489	39.0	35.0	39.0	35.0	39.0
14	37.77896190218485	40.0	35.0	41.0	35.0	41.0
15	37.896433056498	40.0	35.0	41.0	35.0	41.0
16	37.929349749183025	40.0	35.0	41.0	35.0	41.0
17	38.042432473686766	40.0	35.0	41.0	35.0	41.0
18	38.0280668706506	39.0	35.0	41.0	35.0	41.0
19	37.885645845041594	39.0	35.0	41.0	35.0	41.0
20	37.780627077132294	39.0	35.0	41.0	35.0	41.0
21	37.71707810364327	39.0	35.0	41.0	35.0	41.0
22	37.697916449847014	39.0	35.0	41.0	35.0	41.0
23	37.65948386514858	39.0	35.0	41.0	35.0	41.0
24	37.60404394674215	39.0	35.0	41.0	35.0	41.0
25	37.56105988385405	38.0	35.0	41.0	35.0	41.0
26	37.52619007972025	38.0	35.0	41.0	35.0	41.0
27	37.4971952209479	38.0	35.0	41.0	35.0	41.0
28	37.53732767173851	38.0	35.0	41.0	35.0	41.0
29	37.51055824990113	38.0	35.0	41.0	35.0	41.0
30	37.389812251524674	38.0	35.0	41.0	35.0	41.0
31	37.31671280588917	38.0	35.0	41.0	35.0	41.0
32	37.26286174191176	38.0	35.0	41.0	35.0	41.0
33	37.18583178957739	38.0	35.0	41.0	35.0	41.0
34	37.12412665043121	38.0	35.0	41.0	34.0	41.0
35	37.05421879011163	38.0	35.0	41.0	34.0	41.0
36	36.95888405525606	38.0	35.0	41.0	34.0	41.0
37	36.893472167294576	37.0	35.0	41.0	34.0	41.0
38	36.94343782306129	37.0	35.0	41.0	34.0	41.0
39	36.807271610848616	37.0	35.0	41.0	34.0	41.0
40	36.81099743979352	37.0	35.0	41.0	34.0	41.0
41	36.81312053785151	37.0	35.0	41.0	34.0	41.0
42	36.677145126934896	36.0	35.0	41.0	34.0	41.0
43	36.67731337898688	36.0	35.0	41.0	34.0	41.0
44	36.62356985755816	36.0	35.0	41.0	34.0	41.0
45	36.61773827612764	36.0	35.0	41.0	34.0	41.0
46	36.61553191932227	36.0	35.0	41.0	34.0	41.0
47	36.558303672404584	36.0	35.0	41.0	34.0	41.0
48	36.6018896266539	36.0	35.0	41.0	34.0	41.0
49	36.49208521532793	36.0	35.0	41.0	34.0	41.0
50	36.39622837874404	35.0	35.0	40.0	34.0	41.0
51	36.318171568525415	36.0	35.0	40.0	33.0	41.0
52	36.10000069382289	35.0	35.0	40.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	0.0
13	0.0
14	1.0
15	4.0
16	14.0
17	20.0
18	37.0
19	102.0
20	170.0
21	304.0
22	492.0
23	790.0
24	1365.0
25	2223.0
26	2958.0
27	3539.0
28	3586.0
29	3916.0
30	4778.0
31	5841.0
32	7736.0
33	11514.0
34	37785.0
35	175423.0
36	18716.0
37	26658.0
38	49092.0
39	217891.0
40	1560.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.378168168793234	21.47954263194777	25.64872440660797	22.49356479265103
2	24.86158233249381	22.641175613512896	29.05452754129981	23.44271451269349
3	25.552109568511543	25.55193611278785	27.223528922007368	21.67242539669324
4	25.75071637213885	27.82594065038958	24.711543131500253	21.711799845971317
5	22.826252870692226	34.906056380048426	20.978429046201665	21.289261703057676
6	87.44388707338565	2.470876783992118	7.36284855927676	2.722387583345475
7	88.5446370959349	1.7536373665258207	4.972455231077715	4.729270306461573
8	79.40247972302589	3.481776741668922	10.721818648571766	6.393924886733412
9	59.779607157476974	15.482657896745277	12.306510140221608	12.431224805556134
10	53.096705035072745	15.947172324792374	21.107827016075873	9.848295624059004
11	47.345953971789164	15.764696903468419	21.099674597062354	15.789674527680065
12	18.684477100375357	43.131847164692736	24.026913390088044	14.156762344843854
13	11.012530441479509	45.839317555800704	26.41904127552401	16.729110727195774
14	9.527575990952549	21.021446065677274	54.03787579182537	15.413102151544797
15	10.014119295908525	15.762962346231502	54.33084250914112	19.892075848718857
16	11.40540765564182	17.821534875007806	27.306093846484746	43.46696362286563
17	18.07998390330884	21.03410833350679	36.32249581971706	24.56341194346731
18	24.703564168210423	25.34951328323932	27.471917518334273	22.475005030215986
19	27.92966717315738	20.804973322509696	25.29296671731574	25.972392787017185
20	16.92390150490186	31.81351428234429	27.83738872815325	23.4251954846006
21	19.845936626216794	24.050503368510153	24.2763427207571	31.827217284515953
22	15.671551179845833	31.801892748856925	21.616399197940734	30.91015687335651
23	16.665452476600823	21.461850148131187	40.814825607615404	21.05787176765259
24	15.12742057462412	21.686995677483363	38.01559713867439	25.16998660921813
25	12.792879989453892	33.78570586072199	29.518174690728443	23.903239459095673
26	15.390899818912224	32.657202922382034	27.71163332847657	24.24026393022917
27	16.188449236447905	27.475039721360726	40.26219567193278	16.07431537025859
28	14.575311006112578	23.303949933739915	35.335012384738675	26.78572667540883
29	17.803842391191225	28.554107778448472	28.96589166649321	24.67615816386709
30	28.935363459123426	27.205316071019713	20.289809823144545	23.56951064671232
31	31.1155284502078	24.329073260759458	21.899478939006027	22.655919350026714
32	26.41401105953694	28.486460046208606	21.326901595098835	23.77262729915562
33	17.77435491816359	29.034580133075234	24.660720604458504	28.530344344302673
34	19.711161528908132	23.71295853020558	27.709898771239654	28.865981169646638
35	32.67680341915922	20.073163624253272	26.86291447245176	20.387118484135737
36	21.813618355778505	27.71596972156887	26.22373013064685	24.246681792005774
37	19.08169070762997	29.654337433826644	25.464340972323406	25.799630886219983
38	18.023090425937873	26.816948705673386	27.717530823082097	27.442430045306637
39	24.088490171998693	22.057844014736798	25.729901685295815	28.12376412796869
40	13.608989169424612	19.48011850495043	30.55856212143288	36.35233020419208
41	22.4337225679773	22.241360170402903	23.23231271985513	32.09260454176467
42	23.3042968451873	22.473964295873834	26.460497193486393	27.761241665452474
43	23.408890646573553	28.404588944625996	25.452025615941277	22.734494792859174
44	16.318020662045807	35.08402195255639	22.605270278708655	25.992687106689143
45	22.69269196344941	29.44063998223813	24.915873974009394	22.950794080303062
46	23.922319588701786	22.39538885304137	26.02130730109832	27.660984257158518
47	22.946457687210764	18.42412005911371	31.96060473603508	26.66881751764045
48	20.158850751757107	16.934655759770763	28.57683047825212	34.32966301022001
49	20.21002019024624	25.00763205184245	30.043051710620343	24.739296047290967
50	18.13722429212719	28.129835078297916	25.771010691810808	27.961929937764086
51	20.246966259392625	20.03326880780412	30.405053805965487	29.31471112683776
52	22.994331466949745	18.399315890625758	32.59250393744493	26.013848704979566
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	192.0
1	166.5
2	141.0
3	757.5
4	1374.0
5	1024.5
6	675.0
7	797.5
8	920.0
9	976.5
10	1033.0
11	1041.0
12	1049.0
13	1013.0
14	910.0
15	843.0
16	764.5
17	686.0
18	688.5
19	691.0
20	673.0
21	655.0
22	783.0
23	911.0
24	870.0
25	829.0
26	981.5
27	1134.0
28	1610.0
29	2086.0
30	2486.5
31	2887.0
32	3203.0
33	3519.0
34	4271.0
35	5023.0
36	6336.0
37	7649.0
38	7833.5
39	9993.5
40	11969.0
41	21369.0
42	30769.0
43	43681.5
44	56594.0
45	62402.5
46	68211.0
47	66024.5
48	63838.0
49	67172.0
50	70506.0
51	70369.0
52	70232.0
53	60740.0
54	51248.0
55	41752.5
56	32257.0
57	26843.5
58	21430.0
59	19217.5
60	17005.0
61	14733.0
62	12461.0
63	11650.0
64	8598.0
65	6357.0
66	5365.5
67	4374.0
68	3441.0
69	2508.0
70	2214.0
71	1920.0
72	1548.0
73	1176.0
74	944.5
75	713.0
76	545.5
77	378.0
78	316.0
79	254.0
80	196.0
81	138.0
82	90.5
83	43.0
84	23.0
85	3.0
86	1.5
87	0.0
88	0.5
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	576516.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.354336348204875
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.98693774166121	15.198028735615587
2	12.851669564849447	5.745810881772646
3	5.438709355469727	3.6473621469689648
4	2.714520065578259	2.4272517827955022
5	1.6234564309696335	1.8145645552275718
6	0.9429824847236182	1.2647848580386636
7	0.6745985929920212	1.0556142693438586
8	0.5114828754915535	0.9147088188068145
9	0.3647697952083178	0.7338768022577292
>10	4.766724961326344	29.425639019891047
>50	1.855622280952202	26.75338184657619
>100	0.2599889017645285	8.788082054357211
>500	0.0062086901913917265	0.976395731949904
>1k	0.002328258821771897	1.254498496398322
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGCT	2855	0.49521609114057547	TruSeq Adapter, Index 13 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGCTT	2506	0.4346800435720778	TruSeq Adapter, Index 8 (96% over 25bp)
GCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGCT	1870	0.32436220330398463	TruSeq Adapter, Index 13 (95% over 24bp)
ACCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGC	935	0.16218110165199232	TruSeq Adapter, Index 8 (95% over 23bp)
TCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGCT	860	0.14917192237509452	TruSeq Adapter, Index 13 (95% over 24bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	771	0.13373436296650917	No Hit
CGCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGC	714	0.12384738671606685	TruSeq Adapter, Index 8 (95% over 23bp)
GCCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGC	665	0.11534805625516031	TruSeq Adapter, Index 13 (95% over 23bp)
ACTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGCT	580	0.10060431974134283	TruSeq Adapter, Index 8 (95% over 24bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.44491393126990403	0.0
2	0.0	0.0	0.0	1.534562787502862	0.0
3	0.0	0.0	0.0	2.454398490241381	0.0
4	0.0	0.0	0.0	3.018823415135053	0.0
5	0.0	0.0	0.0	5.843376419735098	0.0
6	0.0	0.0	0.0	6.381609530351282	0.0
7	0.0	0.0	0.0	8.995240374941892	0.0
8	0.0	0.0	0.0	11.911724913098682	0.0
9	0.0	0.0	0.0	14.612603986706354	0.0
10	0.0	0.0	0.0	15.760360510376122	0.0
11	0.0	0.0	0.0	16.933094658257534	0.0
12	0.0	0.0	0.0	18.02343733738526	0.0
13	0.0	0.0	0.0	18.3790215709538	0.0
14	0.0	0.0	0.0	18.643021182412976	0.0
15	0.0	0.0	0.0	19.608475740482483	0.0
16	0.0	0.0	0.0	20.67921792283302	0.0
17	0.0	0.0	0.0	22.59677094824775	0.0
18	0.0	0.0	0.0	23.18825496603737	0.0
19	0.0	0.0	0.0	24.002976500218555	0.0
20	0.0	0.0	0.0	24.931484989141673	0.0
21	0.0	0.0	0.0	25.56078235469614	0.0
22	0.0	0.0	0.0	26.442631253946118	0.0
23	0.0	0.0	0.0	26.971671211206626	0.0
24	0.0	0.0	0.0	27.406177799055012	0.0
25	0.0	0.0	0.0	27.917525272498942	0.0
26	0.0	0.0	0.0	28.321156741530157	0.0
27	0.0	0.0	0.0	28.721666007534917	0.0
28	0.0	0.0	0.0	29.120093804855372	0.0
29	0.0	0.0	0.0	29.788592163964225	0.0
30	0.0	0.0	0.0	30.450325749849092	0.0
31	0.0	0.0	0.0	30.874424994275962	0.0
32	0.0	0.0	0.0	31.300952618834515	0.0
33	0.0	0.0	0.0	31.728520977735222	0.0
34	0.0	0.0	0.0	32.44159745783291	0.0
35	0.0	0.0	0.0	32.86205413206225	0.0
36	0.0	0.0	0.0	33.312865557937684	0.0
37	0.0	0.0	0.0	33.708691519402755	0.0
38	0.0	0.0	0.0	34.08942683290663	0.0
39	0.0	0.0	0.0	34.559838755559255	0.0
40	0.0	0.0	0.0	34.99590644492087	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCGAGG	35	1.0183794E-7	46.000004	1
ATAGCGG	55	1.8189894E-12	46.000004	1
TAAACGG	35	1.0183794E-7	46.000004	1
ATAGAGG	140	0.0	46.000004	1
GTAGGGT	35	1.0183794E-7	46.000004	3
TCAGGGT	35	1.0183794E-7	46.000004	3
GTTACGG	35	1.0183794E-7	46.000004	1
CATAGAC	35	1.0183794E-7	46.000004	16
GGTATGC	70	0.0	46.000004	7
CCGACGG	35	1.0183794E-7	46.000004	1
TTACGGG	145	0.0	46.000004	2
TCTTTAC	55	1.8189894E-12	46.000004	10
ACGTAGG	35	1.0183794E-7	46.000004	1
TCATAGA	35	1.0183794E-7	46.000004	15
CGTCAGA	35	1.0183794E-7	46.000004	30
TAGTAGG	115	0.0	46.000004	1
CTTGTAG	20	6.308824E-4	46.0	14
TTCTAGA	25	3.415021E-5	46.0	39
TTAGGGT	60	0.0	46.0	3
AGCCGTG	20	6.308824E-4	46.0	30
>>END_MODULE