FastQCFastQC Report
Thu 26 May 2016
SRR1544771_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544771_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences415657
Sequences flagged as poor quality0
Sequence length52
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGCT36120.8689857262117563TruSeq Adapter, Index 13 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGCTT30270.7282446825146696TruSeq Adapter, Index 8 (96% over 25bp)
GCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGCT18630.44820609300456865TruSeq Adapter, Index 13 (95% over 24bp)
TGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGCTTG13360.3214188621868512TruSeq Adapter, Index 13 (96% over 26bp)
TCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGCT8450.20329261867356982TruSeq Adapter, Index 13 (95% over 24bp)
ACCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGC8390.2018491207894971TruSeq Adapter, Index 8 (95% over 23bp)
GCCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGC7180.17273858012736462TruSeq Adapter, Index 13 (95% over 23bp)
CGCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGC6830.1643181758036073TruSeq Adapter, Index 8 (95% over 23bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA6490.15613835446052876No Hit
AGCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGC5360.12895247764382653TruSeq Adapter, Index 8 (95% over 23bp)
ACTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGCT5220.1255843159143236TruSeq Adapter, Index 8 (95% over 24bp)
CTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGCTTGAAA4690.1128334179383482TruSeq Adapter, Index 8 (96% over 26bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGAGG351.0174699E-746.0000041
AAATAGG351.0174699E-746.0000041
GGGTATA351.0174699E-746.0000046
CTATAGG351.0174699E-746.0000041
ATAGCGG351.0174699E-746.0000041
ATCACTA351.0174699E-746.00000433
CACTATC351.0174699E-746.00000435
TCATGTG351.0174699E-746.00000421
GCATAGG1400.046.0000041
CTTAAGG405.5970304E-946.01
ATGGGTT206.3067826E-446.04
CGGGTGG301.858516E-646.05
AACCGTT206.3067826E-446.040
AGCGTAT301.858516E-646.029
ACTATGC206.3067826E-446.027
TGATAAT206.3067826E-446.045
AATCCGG253.4133664E-546.01
CTGTCGG253.4133664E-546.01
GTACCGG206.3067826E-446.01
AGATCGA253.4133664E-546.012
Produced by FastQC (version 0.11.5)