FastQCFastQC Report
Thu 26 May 2016
SRR1544770_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544770_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2524000
Sequences flagged as poor quality0
Sequence length52
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTGCT102590.40645800316957215No Hit
CTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTGCTT93800.3716323296354992Illumina Single End Adapter 1 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTGCT70580.279635499207607No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC59610.23617274167987323No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48270.19124405705229794No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA30950.12262282091917592No Hit
CGCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTGC30150.11945324881141045No Hit
ACCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTGC28220.1118066561014263No Hit
TCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTGCT27760.10998415213946118No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA26660.10562599049128368No Hit
GCCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTGC25410.10067353407290015No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTAT3100.046.05
GGTCGAC206.312897E-446.026
GCGATCG301.8622959E-646.08
CCAACGA206.312897E-446.014
CGATCGA206.312897E-446.09
TCGAATA206.312897E-446.029
TAATCGA253.4183257E-546.020
GCGATAG2100.044.9047628
GGCGATA3850.044.2077947
CGTTTTT38350.042.4615361
TTACGGG6200.042.290322
TCGTCCC7300.041.2739737
TATACGG950.041.1578941
CTCGTCC7550.041.1258336
GGGCGAT24500.041.1183666
GCGAGAC2700.040.8888920
CGCACTT19050.040.68766434
AAGCTTA21250.040.58823822
AACGCAA21600.040.56944717
ATCAACG22050.040.47165314
Produced by FastQC (version 0.11.5)