##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544770_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2524000
Sequences flagged as poor quality	0
Sequence length	52
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.857332805071316	34.0	31.0	34.0	31.0	34.0
2	33.198906893819334	34.0	33.0	34.0	31.0	34.0
3	33.27003169572108	34.0	34.0	34.0	31.0	34.0
4	36.559002773375596	37.0	37.0	37.0	35.0	37.0
5	36.55642393026941	37.0	37.0	37.0	35.0	37.0
6	36.6550618066561	37.0	37.0	37.0	35.0	37.0
7	36.62343858954041	37.0	37.0	37.0	35.0	37.0
8	36.50990491283677	37.0	37.0	37.0	35.0	37.0
9	38.310982171156894	39.0	39.0	39.0	37.0	39.0
10	38.14030348652932	39.0	39.0	39.0	35.0	39.0
11	37.63331893819334	39.0	37.0	39.0	35.0	39.0
12	37.55883676703645	39.0	37.0	39.0	35.0	39.0
13	37.5574500792393	39.0	37.0	39.0	35.0	39.0
14	38.91434746434231	41.0	37.0	41.0	35.0	41.0
15	38.96578803486529	41.0	37.0	41.0	35.0	41.0
16	38.93749762282092	41.0	37.0	41.0	35.0	41.0
17	38.977772979397784	41.0	37.0	41.0	35.0	41.0
18	38.96429397781299	41.0	37.0	41.0	35.0	41.0
19	38.91479318541997	41.0	37.0	41.0	35.0	41.0
20	38.844255942947704	41.0	36.0	41.0	35.0	41.0
21	38.78631378763867	41.0	36.0	41.0	35.0	41.0
22	38.75429318541997	41.0	36.0	41.0	35.0	41.0
23	38.703686608557845	41.0	35.0	41.0	35.0	41.0
24	38.62686370839937	41.0	35.0	41.0	35.0	41.0
25	38.58991402535658	40.0	35.0	41.0	35.0	41.0
26	38.5100087163233	40.0	35.0	41.0	35.0	41.0
27	38.48692076069731	40.0	36.0	41.0	35.0	41.0
28	38.50212995245642	40.0	36.0	41.0	35.0	41.0
29	38.47976267828843	40.0	36.0	41.0	35.0	41.0
30	38.419610142630745	40.0	35.0	41.0	35.0	41.0
31	38.35843026941363	40.0	35.0	41.0	35.0	41.0
32	38.273886687797145	40.0	35.0	41.0	35.0	41.0
33	38.18902099841522	40.0	35.0	41.0	35.0	41.0
34	38.05240530903328	40.0	35.0	41.0	35.0	41.0
35	38.016764263074485	40.0	35.0	41.0	35.0	41.0
36	37.94880824088748	40.0	35.0	41.0	35.0	41.0
37	37.87549564183835	40.0	35.0	41.0	35.0	41.0
38	37.80960736925515	40.0	35.0	41.0	35.0	41.0
39	37.70743026941363	40.0	35.0	41.0	34.0	41.0
40	37.595400158478604	40.0	35.0	41.0	34.0	41.0
41	37.557819334389855	40.0	35.0	41.0	34.0	41.0
42	37.50073415213946	40.0	35.0	41.0	34.0	41.0
43	37.51165213946117	39.0	35.0	41.0	34.0	41.0
44	37.46953843106181	39.0	35.0	41.0	34.0	41.0
45	37.43426664025357	39.0	35.0	41.0	34.0	41.0
46	37.370621236133125	39.0	35.0	41.0	34.0	41.0
47	37.27920443740095	39.0	35.0	41.0	34.0	41.0
48	37.24025118858954	39.0	35.0	41.0	34.0	41.0
49	37.167653724247224	39.0	35.0	41.0	34.0	41.0
50	37.07906735340729	39.0	35.0	41.0	34.0	41.0
51	36.99018423137876	38.0	35.0	41.0	34.0	41.0
52	36.71179358161648	37.0	35.0	40.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	2.0
13	6.0
14	12.0
15	28.0
16	47.0
17	95.0
18	182.0
19	336.0
20	660.0
21	999.0
22	1667.0
23	2699.0
24	4570.0
25	7460.0
26	10852.0
27	12908.0
28	13911.0
29	15410.0
30	18042.0
31	22101.0
32	29250.0
33	41286.0
34	123508.0
35	393012.0
36	109614.0
37	172580.0
38	289744.0
39	1243548.0
40	9469.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.96576862123613	21.733993660855784	23.664500792393024	20.635736925515054
2	28.095839936608556	23.45657686212361	25.958755942947704	22.488827258320125
3	28.16283676703645	24.373851030110934	24.766640253565768	22.696671949286845
4	26.713034865293185	26.807012678288434	22.229912836767035	24.250039619651346
5	25.291125198098257	35.446156893819335	19.75174326465927	19.510974643423136
6	87.08748019017433	3.0776941362916004	7.123771790808241	2.711053882725832
7	87.41965134706815	3.0862519809825675	5.448969889064976	4.04512678288431
8	77.01957210776546	5.739421553090333	10.583438985736926	6.6575673534072894
9	49.16786846275753	22.823573692551506	14.023058637083993	13.985499207606972
10	37.934786053882725	23.43125990491284	23.56255942947702	15.071394611727417
11	37.55419968304279	18.29583993660856	24.80677496038035	19.343185419968304
12	23.23755942947702	27.963708399366084	27.364896988906494	21.433835182250398
13	17.261133122028525	30.576782884310617	28.913193343898573	23.248890649762284
14	16.628724247226625	26.05542789223455	36.79968304278922	20.516164817749605
15	18.941996830427893	19.014342313787637	40.92606973058637	21.1175911251981
16	19.066600633914423	22.464778129952457	28.181735340729002	30.28688589540412
17	21.07618858954041	23.93300316957211	29.04421553090333	25.946592709984152
18	24.437638668779716	28.4047147385103	25.73026941362916	21.427377179080825
19	29.243026941362913	22.735776545166402	24.722068145800318	23.299128367670367
20	23.868423137876388	27.667947702060218	26.04286846275753	22.420760697305862
21	21.85	24.771434231378763	25.995839936608554	27.382725832012678
22	22.37282091917591	27.285736925515053	23.960142630744848	26.381299524564184
23	22.789263074484943	22.025198098256734	32.52238510301109	22.663153724247227
24	22.37610935023772	21.894770206022187	29.40526941362916	26.323851030110934
25	20.540372424722662	29.407171156893817	26.586885895404123	23.465570522979398
26	19.86584786053883	28.297741679873216	26.975594294770204	24.860816164817752
27	22.400713153724247	26.06537242472266	29.96133122028526	21.57258320126783
28	20.577773375594294	26.04136291600634	27.538906497622822	25.841957210776545
29	22.37923930269414	27.631101426307445	26.28851030110935	23.701148969889065
30	26.31541204437401	25.134469096671953	22.48553882725832	26.064580031695723
31	28.548256735340725	26.91973058637084	23.098335974643422	21.43367670364501
32	24.36295562599049	28.568660855784465	23.4364500792393	23.631933438985737
33	22.42444532488114	25.55146592709984	25.72963549920761	26.29445324881141
34	22.610419968304278	23.992234548335976	28.301901743264658	25.095443740095085
35	25.686489698890654	25.627377179080824	23.307527733755943	25.378605388272586
36	24.003605388272582	27.344057052297938	26.71747226624406	21.93486529318542
37	23.14619651347068	26.241561014263077	26.872226624405705	23.740015847860537
38	22.62052297939778	24.724405705229792	25.83866877971474	26.816402535657684
39	26.314223454833595	22.664896988906495	28.440966719492867	22.579912836767036
40	18.490055467511887	21.864778129952455	32.619809825673535	27.025356576862126
41	21.701941362916006	25.60903328050713	24.354793977812996	28.334231378763867
42	22.953050713153726	23.214817749603803	26.95954833597464	26.87258320126783
43	23.837916006339142	26.527812995245643	26.69013470681458	22.944136291600632
44	21.288431061806655	27.294413629160065	25.747305863708398	25.669849445324882
45	22.57527733755943	25.41053882725832	25.18728209191759	26.82690174326466
46	23.616402535657684	23.709191759112517	27.45824088748019	25.216164817749604
47	22.358557844690967	21.376862123613314	31.010459587955623	25.254120443740096
48	21.149009508716325	21.63022979397781	28.451862123613314	28.768898573692553
49	20.998692551505545	24.049247226624406	30.400237717908084	24.551822503961965
50	19.764025356576862	24.57618858954041	28.63795562599049	27.021830427892233
51	21.426505546751187	22.050237717908082	28.670047543581617	27.853209191759117
52	22.126347068145797	20.210538827258322	31.590213946117274	26.072900158478607
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	364.0
1	966.0
2	1568.0
3	5517.0
4	9466.0
5	6455.0
6	3444.0
7	3558.0
8	3672.0
9	3687.0
10	3702.0
11	3517.0
12	3332.0
13	3241.5
14	3171.5
15	3192.0
16	3100.0
17	3008.0
18	3345.0
19	3682.0
20	3723.0
21	3764.0
22	4239.5
23	4715.0
24	6383.5
25	8052.0
26	9462.0
27	10872.0
28	15312.0
29	19752.0
30	22239.5
31	24727.0
32	26754.5
33	28782.0
34	33874.0
35	38966.0
36	42292.5
37	45619.0
38	50650.0
39	65412.5
40	75144.0
41	95208.5
42	115273.0
43	140977.0
44	166681.0
45	185570.5
46	204460.0
47	222928.5
48	241397.0
49	251127.5
50	260858.0
51	237689.0
52	214520.0
53	191569.0
54	168618.0
55	151993.0
56	135368.0
57	124916.0
58	114464.0
59	115042.0
60	115620.0
61	120558.0
62	125496.0
63	116531.5
64	88151.0
65	68735.0
66	55475.5
67	42216.0
68	35963.0
69	29710.0
70	25158.5
71	20607.0
72	18223.5
73	15840.0
74	12195.5
75	8551.0
76	7613.0
77	6675.0
78	4954.0
79	3233.0
80	2462.0
81	1691.0
82	1366.5
83	1042.0
84	763.5
85	485.0
86	287.5
87	90.0
88	104.5
89	71.0
90	23.0
91	12.5
92	2.0
93	3.0
94	4.0
95	2.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	2524000.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.43596920036333
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.53119904140308	13.12534304632316
2	13.054638779499689	5.074591144804494
3	5.523757546890908	3.2207874465493864
4	2.816949587225738	2.190005816651826
5	1.6730535632526455	1.6258708762968272
6	1.073438468951125	1.251799021261154
7	0.7283188815303283	0.9908908344626574
8	0.5303863774492446	0.824685863711664
9	0.3981784715304844	0.6965086058022819
>10	3.804244324932059	18.244849509140757
>50	2.3185662909488403	32.66617280860635
>100	0.5286095285619228	14.863105161620691
>500	0.010662364470856138	1.3969921469180868
>1k	0.007176591470768555	2.5268923394616367
>5k	6.151364117801618E-4	0.8926578164779547
>10k+	2.0504547059338726E-4	0.4088475619111272
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTGCT	10259	0.40645800316957215	No Hit
CTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTGCTT	9380	0.3716323296354992	Illumina Single End Adapter 1 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTGCT	7058	0.279635499207607	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	5961	0.23617274167987323	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4827	0.19124405705229794	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA	3095	0.12262282091917592	No Hit
CGCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTGC	3015	0.11945324881141045	No Hit
ACCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTGC	2822	0.1118066561014263	No Hit
TCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTGCT	2776	0.10998415213946118	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA	2666	0.10562599049128368	No Hit
GCCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTGC	2541	0.10067353407290015	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.3809429477020602	0.0
2	0.0	0.0	0.0	1.2771394611727416	0.0
3	0.0	0.0	0.0	2.0194136291600633	0.0
4	0.0	0.0	0.0	2.4984548335974646	0.0
5	0.0	0.0	0.0	4.802892234548336	0.0
6	0.0	0.0	0.0	5.181101426307449	0.0
7	0.0	0.0	0.0	6.494374009508716	0.0
8	0.0	0.0	0.0	8.18906497622821	0.0
9	0.0	0.0	0.0	9.346077654516641	0.0
10	0.0	0.0	0.0	10.269572107765452	0.0
11	0.0	0.0	0.0	11.150633914421553	0.0
12	0.0	0.0	0.0	11.9270206022187	0.0
13	0.0	0.0	0.0	12.280744849445325	0.0
14	0.0	0.0	0.0	12.536133122028525	0.0
15	0.0	0.0	0.0	13.320206022187005	0.0
16	3.961965134706815E-5	0.0	0.0	14.18637083993661	0.0
17	3.961965134706815E-5	0.0	0.0	15.174841521394612	0.0
18	3.961965134706815E-5	0.0	0.0	15.74988114104596	0.0
19	3.961965134706815E-5	0.0	0.0	16.37686212361331	0.0
20	7.92393026941363E-5	0.0	0.0	17.16715530903328	0.0
21	7.92393026941363E-5	0.0	0.0	17.8973058637084	0.0
22	1.1885895404120444E-4	0.0	0.0	18.692551505546753	0.0
23	1.1885895404120444E-4	0.0	0.0	19.27809033280507	0.0
24	1.9809825673534074E-4	0.0	0.0	19.779358161648176	0.0
25	1.9809825673534074E-4	0.0	0.0	20.256576862123612	0.0
26	1.9809825673534074E-4	0.0	0.0	20.712599049128368	0.0
27	1.9809825673534074E-4	0.0	0.0	21.183042789223453	0.0
28	1.9809825673534074E-4	0.0	0.0	21.65958795562599	0.0
29	1.9809825673534074E-4	0.0	0.0	22.20919175911252	0.0
30	1.9809825673534074E-4	0.0	0.0	22.776109350237718	0.0
31	2.3771790808240888E-4	0.0	0.0	23.311450079239304	0.0
32	2.7733755942947703E-4	0.0	0.0	23.786251980982566	0.0
33	2.7733755942947703E-4	0.0	0.0	24.251782884310618	0.0
34	2.7733755942947703E-4	0.0	0.0	24.836925515055466	0.0
35	2.7733755942947703E-4	0.0	0.0	25.354397781299525	0.0
36	2.7733755942947703E-4	0.0	0.0	25.91255942947702	0.0
37	2.7733755942947703E-4	0.0	0.0	26.39837559429477	0.0
38	2.7733755942947703E-4	0.0	0.0	26.881933438985737	0.0
39	3.565768621236133E-4	0.0	0.0	27.407725832012677	0.0
40	3.565768621236133E-4	0.0	0.0	27.97717908082409	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTAT	310	0.0	46.0	5
GGTCGAC	20	6.312897E-4	46.0	26
GCGATCG	30	1.8622959E-6	46.0	8
CCAACGA	20	6.312897E-4	46.0	14
CGATCGA	20	6.312897E-4	46.0	9
TCGAATA	20	6.312897E-4	46.0	29
TAATCGA	25	3.4183257E-5	46.0	20
GCGATAG	210	0.0	44.904762	8
GGCGATA	385	0.0	44.207794	7
CGTTTTT	3835	0.0	42.461536	1
TTACGGG	620	0.0	42.29032	2
TCGTCCC	730	0.0	41.27397	37
TATACGG	95	0.0	41.157894	1
CTCGTCC	755	0.0	41.12583	36
GGGCGAT	2450	0.0	41.118366	6
GCGAGAC	270	0.0	40.88889	20
CGCACTT	1905	0.0	40.687664	34
AAGCTTA	2125	0.0	40.588238	22
AACGCAA	2160	0.0	40.569447	17
ATCAACG	2205	0.0	40.471653	14
>>END_MODULE