##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544769_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1730772
Sequences flagged as poor quality	0
Sequence length	52
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.854889609954405	34.0	33.0	34.0	31.0	34.0
2	33.226313460120686	34.0	33.0	34.0	31.0	34.0
3	33.32959742819967	34.0	34.0	34.0	31.0	34.0
4	36.56338269858768	37.0	37.0	37.0	35.0	37.0
5	36.57445694753555	37.0	37.0	37.0	35.0	37.0
6	36.50157906414017	37.0	37.0	37.0	35.0	37.0
7	36.37018278548532	37.0	37.0	37.0	35.0	37.0
8	36.40673814921896	37.0	37.0	37.0	35.0	37.0
9	38.26197558083907	39.0	39.0	39.0	37.0	39.0
10	38.15890250131155	39.0	39.0	39.0	35.0	39.0
11	37.72215288899982	39.0	37.0	39.0	35.0	39.0
12	37.68888623111536	39.0	37.0	39.0	35.0	39.0
13	37.68942471914267	39.0	37.0	39.0	35.0	39.0
14	39.01983565715184	41.0	38.0	41.0	35.0	41.0
15	39.07040037624829	41.0	38.0	41.0	35.0	41.0
16	39.05928972735866	41.0	38.0	41.0	35.0	41.0
17	39.11154155486685	41.0	38.0	41.0	35.0	41.0
18	39.111131911077834	41.0	38.0	41.0	35.0	41.0
19	39.072081129114636	41.0	38.0	41.0	35.0	41.0
20	39.011840958832245	41.0	38.0	41.0	35.0	41.0
21	38.95927828737696	41.0	37.0	41.0	35.0	41.0
22	38.932585574529746	41.0	37.0	41.0	35.0	41.0
23	38.891989817260736	41.0	37.0	41.0	35.0	41.0
24	38.797329746494626	41.0	36.0	41.0	35.0	41.0
25	38.75352559435905	41.0	36.0	41.0	35.0	41.0
26	38.661697207951136	41.0	36.0	41.0	35.0	41.0
27	38.65391397595986	40.0	36.0	41.0	35.0	41.0
28	38.71179508335009	41.0	36.0	41.0	35.0	41.0
29	38.70750277910666	41.0	36.0	41.0	35.0	41.0
30	38.69052422849457	41.0	36.0	41.0	35.0	41.0
31	38.659351433926595	41.0	36.0	41.0	35.0	41.0
32	38.59476927059139	40.0	36.0	41.0	35.0	41.0
33	38.54724076885921	41.0	36.0	41.0	35.0	41.0
34	38.432235441756625	40.0	36.0	41.0	35.0	41.0
35	38.37449242303435	40.0	35.0	41.0	35.0	41.0
36	38.343955760781895	40.0	35.0	41.0	35.0	41.0
37	38.28169337151283	40.0	35.0	41.0	35.0	41.0
38	38.200006124434644	40.0	35.0	41.0	35.0	41.0
39	38.16576475699861	40.0	35.0	41.0	35.0	41.0
40	38.09929210779929	40.0	35.0	41.0	35.0	41.0
41	38.06012114825061	40.0	35.0	41.0	35.0	41.0
42	38.009746517738904	40.0	35.0	41.0	35.0	41.0
43	37.93748801113029	40.0	35.0	41.0	35.0	41.0
44	37.79743027966711	40.0	35.0	41.0	35.0	41.0
45	37.77106458851888	40.0	35.0	41.0	35.0	41.0
46	37.706906513394024	40.0	35.0	41.0	35.0	41.0
47	37.62451842299274	39.0	35.0	41.0	35.0	41.0
48	37.55087671859725	39.0	35.0	41.0	35.0	41.0
49	37.496758094075936	39.0	35.0	41.0	35.0	41.0
50	37.41615533415146	39.0	35.0	41.0	35.0	41.0
51	37.326928676914115	39.0	35.0	41.0	34.0	41.0
52	36.88403787442829	38.0	35.0	40.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	3.0
13	7.0
14	11.0
15	22.0
16	38.0
17	74.0
18	122.0
19	247.0
20	363.0
21	632.0
22	979.0
23	1562.0
24	2488.0
25	4006.0
26	5725.0
27	6785.0
28	7277.0
29	8053.0
30	9507.0
31	11948.0
32	16140.0
33	23844.0
34	75385.0
35	258393.0
36	69992.0
37	114621.0
38	198810.0
39	907905.0
40	5831.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.994178320425796	21.305117022923874	22.593501628175172	23.107203028475155
2	27.643155770950766	23.79175304430624	25.870074163436897	22.6950170213061
3	28.375950154035305	24.130965834899108	24.75583150178071	22.737252509284875
4	26.60685520680945	26.126318197890885	22.892732260517274	24.3740943347824
5	25.02825328812807	34.500962576237654	19.98148802961915	20.48929610601512
6	84.82006873233448	3.0889106132985744	8.30854670632527	3.7824739480416834
7	85.36809007772254	3.067012870557185	6.46896298299256	5.095934068727712
8	73.69982874694068	5.729813054521335	12.348015798730277	8.222342399807715
9	47.84206123047981	21.802062894477146	15.126775797158725	15.22910007788432
10	36.326275211292995	22.665954845583357	25.62180344955893	15.385966493564721
11	35.48341433764817	18.145255412035784	25.416692666625067	20.954637583690978
12	22.4206307936574	26.675668430041622	28.74058512617491	22.16311565012607
13	16.865306348843177	29.490366148747494	29.67583251866797	23.96849498374136
14	16.08553870758251	25.82304312757544	36.02565791450289	22.065760250339157
15	18.11694434622238	19.083218355739522	40.69189933740551	22.107937960632597
16	18.257575232324072	23.450749145468034	28.150443848178732	30.14123177402916
17	20.041923488477977	24.29378335216886	29.23608655559484	26.428206603758326
18	23.3209226865237	28.429567846024778	26.364246706094157	21.88526276135736
19	27.70578678185226	22.449750747065472	25.25179515268331	24.59266731839896
20	22.745514718287563	28.227692613469596	26.54780641239863	22.478986255844212
21	21.31002812617722	24.603587300926986	26.08414048759744	28.00224408529835
22	21.460192330358936	27.376627308507416	23.966241654013352	27.19693870712029
23	21.79518734992246	22.11082684489927	32.790569757310614	23.303416047867657
24	22.4741329302762	22.21638667600354	28.64132306277199	26.668157330948272
25	20.214216546142417	29.069686821834416	26.31715789254737	24.398938739475796
26	19.667004088349014	27.84364433905795	26.47546875036111	26.013882822231928
27	23.064678652069713	25.637172313857633	28.992553611914225	22.305595422158436
28	21.063490742859255	25.56518131793211	26.697450617412343	26.673877321796287
29	22.743550276986223	27.781071105841786	25.430328200363768	24.045050416808223
30	26.00787394295725	25.054079913472137	21.734636335692976	27.203409807877645
31	28.62300753652127	26.146887053869605	22.765159131300948	22.464946278308176
32	23.363273729873143	28.784900610825687	23.1901140069287	24.661711652372468
33	22.70784366745013	24.713769346857934	25.662825606145695	26.915561379546237
34	22.81750571421308	23.211491750502088	28.812460566729758	25.15854196855507
35	25.523408051436007	24.885600183039706	23.388638133734542	26.202353631789745
36	24.496005250836042	26.454957672067724	26.905161396186212	22.14387568091002
37	23.198896215099392	25.459852597569178	27.58618697321195	23.75506421411948
38	22.270697700217013	23.979703854696055	25.85586085284486	27.893737592242076
39	26.680579533294967	21.74832964711701	28.77825617701234	22.79283464257568
40	18.633187964677035	20.757442343647806	32.95003616883102	27.659333522844143
41	21.595854335521953	25.129710903573667	24.447414217470588	28.827020543433797
42	22.539479492388367	22.055649155405796	27.58035142699327	27.824519925212567
43	24.745142629994014	25.48047923123323	26.43716214498501	23.337215993787744
44	21.38889466665742	25.88942968802361	26.472002089241105	26.24967355607787
45	22.336968705294517	24.047650412648228	25.567839091457458	28.0475417905998
46	23.96421943502668	22.134977917368666	27.783093324828457	26.117709322776193
47	21.540965534455143	20.502353862900485	32.050090942076714	25.906589660567654
48	21.04280633150987	20.24778538132117	28.40767010328339	30.301738183885575
49	21.079437384011296	22.327088721102488	31.16158569701844	25.431888197867774
50	20.013265756552567	22.706341447631463	29.542308287862294	27.738084507953676
51	21.287205940470496	20.518704947849862	29.026642446260976	29.167446665418666
52	21.6668631107968	19.367311234524248	33.150755847679534	25.815069806999418
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	189.0
1	466.0
2	743.0
3	2564.5
4	4386.0
5	3195.5
6	2005.0
7	2000.5
8	1996.0
9	2062.0
10	2128.0
11	2013.5
12	1899.0
13	1839.5
14	1816.0
15	1852.0
16	1848.0
17	1844.0
18	2120.5
19	2397.0
20	2388.0
21	2379.0
22	2715.0
23	3051.0
24	3860.5
25	4670.0
26	5606.0
27	6542.0
28	8895.0
29	11248.0
30	12756.0
31	14264.0
32	15737.0
33	17210.0
34	20099.5
35	22989.0
36	25520.5
37	28052.0
38	31231.5
39	40363.5
40	46316.0
41	59098.0
42	71880.0
43	89830.5
44	107781.0
45	123017.0
46	138253.0
47	161755.0
48	185257.0
49	194500.0
50	203743.0
51	182332.5
52	160922.0
53	141207.5
54	121493.0
55	107345.0
56	93197.0
57	84462.5
58	75728.0
59	75149.5
60	74571.0
61	77273.5
62	79976.0
63	77585.0
64	59885.0
65	44576.0
66	36233.5
67	27891.0
68	23891.0
69	19891.0
70	16694.5
71	13498.0
72	11931.5
73	10365.0
74	8004.5
75	5644.0
76	4970.0
77	4296.0
78	3159.5
79	2023.0
80	1555.5
81	1088.0
82	899.0
83	710.0
84	508.0
85	306.0
86	176.0
87	46.0
88	55.5
89	44.5
90	24.0
91	13.5
92	3.0
93	1.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1730772.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.824789108800843
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.33540657654952	15.597412437739047
2	12.753115405343138	5.821743392143126
3	5.2855259734611435	3.6192304702001965
4	2.5690803985279334	2.345548731998164
5	1.4821314779568833	1.6914669207940582
6	0.923216725581228	1.264333623786551
7	0.6623178266877092	1.0582085302002386
8	0.46766251888053373	0.8539438694031019
9	0.38650077204344985	0.7939618751052405
>10	5.6736775100611405	35.41109713001943
>50	1.2506660406313759	17.584263050068007
>100	0.19316172353238442	7.392814141136158
>500	0.009912246072476287	1.5884134164742654
>1k	0.006354003892613005	2.4180941689089805
>5k	7.624804671135606E-4	1.201476581154761
>10k+	5.083203114090404E-4	1.3579916608687075
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTGCT	12696	0.7335454929938778	No Hit
CTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTGCTT	10713	0.6189723429775845	Illumina Single End Adapter 1 (95% over 21bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA	8353	0.48261700559056886	No Hit
GCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTGCT	7057	0.40773712539837714	No Hit
TGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTGCTTG	5301	0.3062795099527841	Illumina Single End Adapter 1 (95% over 22bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	3791	0.2190352050992274	No Hit
CGCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTGC	2812	0.16247085115774926	No Hit
TCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTGCT	2533	0.146350876949708	No Hit
ACCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTGC	2468	0.1425953274030317	No Hit
GCCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTGC	2379	0.1374531134083519	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1966	0.11359092936562414	No Hit
AGCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTGC	1889	0.10914204759494607	No Hit
ACTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTGCT	1793	0.10359538980293187	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.6313945453242831	0.0
2	0.0	0.0	0.0	2.0506456078559165	0.0
3	0.0	0.0	0.0	3.0508928963491435	0.0
4	5.777768533348124E-5	0.0	0.0	3.719322937972188	0.0
5	5.777768533348124E-5	0.0	0.0	6.807944662844095	0.0
6	5.777768533348124E-5	0.0	0.0	7.323206060648081	0.0
7	5.777768533348124E-5	0.0	0.0	9.12910539343137	0.0
8	5.777768533348124E-5	0.0	0.0	11.282421948124883	0.0
9	5.777768533348124E-5	0.0	0.0	12.797930634422096	0.0
10	5.777768533348124E-5	0.0	0.0	14.028017555171912	0.0
11	1.1555537066696248E-4	0.0	0.0	15.139371332561424	0.0
12	1.1555537066696248E-4	0.0	0.0	16.151405268862682	0.0
13	1.1555537066696248E-4	0.0	0.0	16.604844543359842	0.0
14	1.1555537066696248E-4	0.0	0.0	16.92579958538733	0.0
15	1.1555537066696248E-4	0.0	0.0	17.87127362818442	0.0
16	1.1555537066696248E-4	0.0	0.0	18.89503643460837	0.0
17	1.1555537066696248E-4	0.0	0.0	20.024185739080597	0.0
18	1.1555537066696248E-4	0.0	0.0	20.70740686814901	0.0
19	1.1555537066696248E-4	0.0	0.0	21.417841287009498	0.0
20	1.1555537066696248E-4	0.0	0.0	22.318075402190466	0.0
21	1.1555537066696248E-4	0.0	0.0	23.16908292946731	0.0
22	1.7333305600044375E-4	0.0	0.0	24.050654852285568	0.0
23	1.7333305600044375E-4	0.0	0.0	24.707124913044584	0.0
24	1.7333305600044375E-4	0.0	0.0	25.268781792171353	0.0
25	1.7333305600044375E-4	0.0	0.0	25.79565650472737	0.0
26	1.7333305600044375E-4	0.0	0.0	26.28878904904863	0.0
27	1.7333305600044375E-4	0.0	0.0	26.790414913113917	0.0
28	1.7333305600044375E-4	0.0	0.0	27.30775630758991	0.0
29	1.7333305600044375E-4	0.0	0.0	27.88455094027405	0.0
30	2.888884266674062E-4	0.0	0.0	28.471630000947552	0.0
31	2.888884266674062E-4	0.0	0.0	29.028433554506314	0.0
32	2.888884266674062E-4	0.0	0.0	29.534681633398275	0.0
33	2.888884266674062E-4	0.0	0.0	30.02024530094085	0.0
34	2.888884266674062E-4	0.0	0.0	30.62650655314507	0.0
35	3.466661120008875E-4	0.0	0.0	31.137145736122378	0.0
36	3.466661120008875E-4	0.0	0.0	31.689384852539792	0.0
37	3.466661120008875E-4	0.0	0.0	32.17772184897837	0.0
38	3.466661120008875E-4	0.0	0.0	32.669121062739634	0.0
39	4.044437973343687E-4	0.0	0.0	33.203449096703665	0.0
40	4.044437973343687E-4	0.0	0.0	33.77527484844913	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATACGG	75	0.0	46.000004	1
AATCCGT	20	6.312345E-4	46.0	10
CGACCGA	20	6.312345E-4	46.0	12
TATCGAA	20	6.312345E-4	46.0	31
GGCGATA	245	0.0	45.061226	7
ATCGAGG	150	0.0	44.46667	1
GCGATAG	110	0.0	43.909092	8
CGTTTTT	2020	0.0	43.267326	1
CGGGTAT	295	0.0	42.881355	5
TCGGCGT	1230	0.0	42.260162	4
TTAATCG	300	0.0	42.166668	19
TAATCGT	300	0.0	42.166668	20
GTCCCCC	1220	0.0	42.04098	9
ATAGCGG	290	0.0	42.034485	1
TACGGGA	355	0.0	41.46479	3
GGTATGC	340	0.0	41.264706	7
GTCGGCG	1270	0.0	40.929134	3
ATTACGG	90	0.0	40.88889	1
ATTCGAT	45	1.589251E-8	40.88889	13
GCGTCCC	1285	0.0	40.809338	7
>>END_MODULE