FastQCFastQC Report
Thu 26 May 2016
SRR1544768_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544768_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences586282
Sequences flagged as poor quality0
Sequence length52
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTGCT33290.5678154881098175TruSeq Adapter, Index 14 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTGCTT24770.4224929300234358Illumina PCR Primer Index 8 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTGCT17040.2906451161727633TruSeq Adapter, Index 14 (95% over 23bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10290.17551280782967923No Hit
ACCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTGC8960.1528274789265234No Hit
CGCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTGC8680.14805162021006957No Hit
TCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTGCT8180.13952330107354483TruSeq Adapter, Index 14 (95% over 23bp)
GCCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTGC6550.11172098068847414TruSeq Adapter, Index 14 (95% over 22bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC6240.1064334228238288No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTACGG351.0184158E-746.0000041
ACGGGTC351.0184158E-746.0000044
GCGTAGG1050.046.0000041
GCGAGAC253.4150922E-546.020
CCTTCGC206.308912E-446.015
TCGCCCG206.308912E-446.043
CTTCGAA206.308912E-446.012
CTATGCA206.308912E-446.041
TAGAGGT253.4150922E-546.038
ATCTCGG206.308912E-446.01
AATCCAC206.308912E-446.010
GCCTTCG206.308912E-446.014
CAAACGG301.8598312E-646.01
TGCCCGT206.308912E-446.042
TGCCCAA206.308912E-446.030
ACGCCCG206.308912E-446.09
GTCGAGG405.6024874E-946.01
ATCTACG206.308912E-446.013
GTTTCGA206.308912E-446.030
GAAGACG253.4150922E-546.013
Produced by FastQC (version 0.11.5)