##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544768_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	586282
Sequences flagged as poor quality	0
Sequence length	52
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.91380086715949	34.0	33.0	34.0	31.0	34.0
2	33.212520595890716	34.0	33.0	34.0	31.0	34.0
3	33.24569405166797	34.0	33.0	34.0	31.0	34.0
4	36.54607339130316	37.0	37.0	37.0	35.0	37.0
5	36.53797319378729	37.0	37.0	37.0	35.0	37.0
6	36.670315650147884	37.0	37.0	37.0	35.0	37.0
7	36.64560228695406	37.0	37.0	37.0	35.0	37.0
8	36.55629202329254	37.0	37.0	37.0	35.0	37.0
9	38.34547538556531	39.0	39.0	39.0	37.0	39.0
10	38.04385432266384	39.0	39.0	39.0	35.0	39.0
11	36.978494990465336	39.0	35.0	39.0	35.0	39.0
12	36.855482515240105	39.0	35.0	39.0	35.0	39.0
13	36.83920195400848	39.0	35.0	39.0	35.0	39.0
14	37.98391900143617	40.0	35.0	41.0	35.0	41.0
15	38.076441030084496	40.0	35.0	41.0	35.0	41.0
16	38.078310437639225	40.0	35.0	41.0	35.0	41.0
17	38.18312006849946	40.0	35.0	41.0	35.0	41.0
18	38.156189342330144	40.0	36.0	41.0	35.0	41.0
19	38.010356790759396	40.0	35.0	41.0	35.0	41.0
20	37.91691711497197	40.0	35.0	41.0	35.0	41.0
21	37.85741503235644	40.0	35.0	41.0	35.0	41.0
22	37.84858822205014	40.0	35.0	41.0	35.0	41.0
23	37.82890486148304	39.0	35.0	41.0	35.0	41.0
24	37.773177412917335	39.0	35.0	41.0	35.0	41.0
25	37.74602836177812	39.0	35.0	41.0	35.0	41.0
26	37.690096233553135	39.0	35.0	41.0	35.0	41.0
27	37.71558226246073	39.0	35.0	41.0	35.0	41.0
28	37.73999372315711	39.0	35.0	41.0	35.0	41.0
29	37.741051234730044	39.0	35.0	41.0	35.0	41.0
30	37.60609229005837	39.0	35.0	41.0	35.0	41.0
31	37.5296035013867	39.0	35.0	41.0	35.0	41.0
32	37.40203519807874	39.0	35.0	41.0	35.0	41.0
33	37.3219559870506	39.0	35.0	41.0	35.0	41.0
34	37.22342490473868	38.0	35.0	41.0	34.0	41.0
35	37.0926823610481	38.0	35.0	41.0	34.0	41.0
36	37.05792093224762	38.0	35.0	41.0	34.0	41.0
37	36.96819278094842	38.0	35.0	41.0	34.0	41.0
38	36.92780266151784	37.0	35.0	41.0	34.0	41.0
39	36.79926724681979	37.0	35.0	41.0	34.0	41.0
40	36.76045145510181	37.0	35.0	41.0	34.0	41.0
41	36.72360058811289	37.0	35.0	41.0	34.0	41.0
42	36.58231021931425	36.0	35.0	41.0	34.0	41.0
43	36.585955222913206	36.0	35.0	41.0	34.0	41.0
44	36.50022173629755	36.0	35.0	41.0	34.0	41.0
45	36.40906935570255	36.0	35.0	40.0	34.0	41.0
46	36.28417894460345	36.0	35.0	40.0	34.0	41.0
47	36.11930265640085	36.0	35.0	40.0	33.0	41.0
48	36.04580901340993	36.0	35.0	39.0	34.0	41.0
49	35.86558004509775	35.0	35.0	39.0	33.0	41.0
50	35.700065838623736	35.0	35.0	39.0	33.0	41.0
51	35.576582600182164	35.0	35.0	39.0	33.0	41.0
52	35.30681822058327	35.0	35.0	37.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	2.0
15	2.0
16	12.0
17	27.0
18	40.0
19	96.0
20	183.0
21	288.0
22	501.0
23	794.0
24	1429.0
25	2342.0
26	3244.0
27	3669.0
28	3792.0
29	4150.0
30	4745.0
31	5915.0
32	7699.0
33	10962.0
34	34773.0
35	171483.0
36	20681.0
37	32145.0
38	66410.0
39	209299.0
40	1598.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.621131128023716	21.720605442432138	25.24484804240962	21.413415387134517
2	26.686304542865035	23.648517266434922	27.34997833806939	22.315199852630645
3	25.947069840111077	24.83685325491828	27.001340651768263	22.214736253202382
4	25.84234208111455	26.686304542865035	24.011653095268148	23.459700280752266
5	24.169768132059318	34.775244677476024	21.249842226095975	19.805144964368683
6	87.37911107623975	2.4651959296038424	7.451874695112591	2.703818299043805
7	88.01719991403453	2.2345901801522134	5.402178473840234	4.346031431973009
8	79.2662916480465	4.029801358390673	10.77723689282632	5.926670100736506
9	57.770492698053154	15.235501004635996	13.511074875230692	13.48293142208016
10	51.292552048331686	16.40166336336439	20.573717084952293	11.73206750335163
11	45.528261143954616	16.505026591299067	22.22838156382082	15.738330700925493
12	19.1390832398061	41.526262105949016	24.503566543062895	14.831088111181993
13	12.388918643246766	44.448917074036046	27.357312692526804	15.804851590190387
14	10.61246976710866	21.925285101708734	52.11502314585814	15.347221985324468
15	12.732609904448713	15.534674439945281	52.69426658161089	19.03844907399511
16	13.633029838882996	18.12250759873235	26.47753129040291	41.76693127198175
17	19.564987497484147	21.131639722863742	35.63899283962325	23.66437994002886
18	27.29386199815106	24.617675453109598	26.6066500421299	21.481812506609447
19	29.814321435759584	20.5111192224902	25.04647933929406	24.62808000245616
20	18.698851405978694	30.673805438338547	27.847691042876978	22.77965211280578
21	20.119157674975526	23.549418198068505	25.937688689060895	30.393735437895074
22	16.666382389362116	31.34379018970393	23.0129869243811	28.976840496552853
23	17.762271398405545	21.92409113702962	40.317458151538	19.996179313026836
24	16.584169392886018	21.033734619176435	38.548514196240035	23.83358179169751
25	14.250650710750119	34.053919444908765	27.997618893297084	23.697810951044037
26	16.620329466024884	32.379980964791685	27.69366959927134	23.30601996991209
27	17.345407159012215	26.92373294762589	38.77826711377801	16.952592779583885
28	14.844562855417701	24.254710190659104	35.42100900249368	25.479717951429514
29	21.692120856516148	27.934168198921338	28.179272090905062	22.194438853657456
30	30.645661985188017	26.301847916190503	20.73831364428722	22.314176454334262
31	34.24836512122153	24.210704063914633	22.06924312873327	19.471687686130565
32	29.711811039738556	27.433726431990067	22.819052947216527	20.03540958105485
33	20.132802985593965	27.330704336820848	25.231373298173914	27.305119379411273
34	23.269007064859572	24.052759593506195	29.06297651983175	23.615256821802475
35	33.29438734260987	19.892645518709426	26.558038623051704	20.254928515628997
36	24.11228726107914	28.981275222503843	26.092392398197457	20.81404511821956
37	22.226675899993516	29.357715229190052	25.079910350309238	23.335698520507194
38	20.912632487437786	28.597330294977503	26.862499616225637	23.627537601359073
39	27.72948853964474	23.477098051790776	24.917531153949806	23.875882254614673
40	16.677469204239596	21.456909814730796	29.62328708710143	32.242333893928176
41	24.813997359632396	24.720185849130623	22.526361034450996	27.939455756785986
42	24.21974408219935	23.53713741851191	25.891465199340928	26.35165329994781
43	23.928928399643855	27.297785024953864	27.19425123063645	21.579035344765828
44	19.139936071719752	33.08527295738229	22.52294970679639	25.251841264101575
45	24.236289021324207	27.76513691363542	25.1842969765403	22.814277088500074
46	25.981865382188097	23.596665086084855	26.04002851869919	24.38144101302786
47	23.826588570005562	19.933581450564745	32.360195264394946	23.879634715034744
48	21.56931306095019	19.130554920669578	29.91239710582962	29.387734912550616
49	21.91999754384409	26.27677465792912	29.455449766494624	22.347778031732172
50	19.843181267717583	28.545307548244704	25.488416836948772	26.123094347088944
51	22.02012001050689	21.79991881040182	29.715222367393167	26.464738811698123
52	25.461979047625544	19.39407998198819	31.771570677592013	23.372370292794255
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	166.0
1	179.5
2	193.0
3	999.0
4	1805.0
5	1284.5
6	764.0
7	853.5
8	943.0
9	1013.5
10	1084.0
11	1081.0
12	1078.0
13	1094.5
14	1049.0
15	987.0
16	928.0
17	869.0
18	831.5
19	794.0
20	901.0
21	1008.0
22	1035.5
23	1063.0
24	1185.0
25	1307.0
26	1442.5
27	1578.0
28	1911.0
29	2244.0
30	2622.5
31	3001.0
32	3545.0
33	4089.0
34	4972.5
35	5856.0
36	7196.0
37	8536.0
38	9093.0
39	11836.0
40	14022.0
41	23038.5
42	32055.0
43	44762.5
44	57470.0
45	61973.5
46	66477.0
47	63042.0
48	59607.0
49	62713.5
50	65820.0
51	66429.0
52	67038.0
53	58937.5
54	50837.0
55	42333.5
56	33830.0
57	29009.0
58	24188.0
59	21499.0
60	18810.0
61	17267.0
62	15724.0
63	13999.5
64	9361.0
65	6447.0
66	5624.5
67	4802.0
68	4231.5
69	3661.0
70	2724.5
71	1788.0
72	1480.5
73	1173.0
74	958.0
75	743.0
76	844.0
77	945.0
78	570.5
79	196.0
80	163.0
81	130.0
82	119.0
83	108.0
84	57.0
85	6.0
86	4.0
87	2.0
88	1.0
89	1.0
90	2.0
91	1.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	586282.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.930873999911046
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.02661410823056	16.518672048645477
2	12.788769702655802	6.120928727362718
3	5.011586751312281	3.5979495325583324
4	2.535659326330628	2.4272217538047034
5	1.4057725812371091	1.682068325705749
6	0.8985238729811839	1.2901476954134874
7	0.5868954459249671	0.9831444677286398
8	0.45589415661840005	0.8727956495464515
9	0.3366513615374835	0.7250725183366817
>10	5.434693309102016	36.36838954082619
>50	1.3092829620430424	19.77603078209016
>100	0.20181213412754956	7.328102808986077
>500	0.00499181957205953	0.8522518205928243
>1k	0.00285246832689116	1.4572243284025084
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTGCT	3329	0.5678154881098175	TruSeq Adapter, Index 14 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTGCTT	2477	0.4224929300234358	Illumina PCR Primer Index 8 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTGCT	1704	0.2906451161727633	TruSeq Adapter, Index 14 (95% over 23bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1029	0.17551280782967923	No Hit
ACCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTGC	896	0.1528274789265234	No Hit
CGCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTGC	868	0.14805162021006957	No Hit
TCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTGCT	818	0.13952330107354483	TruSeq Adapter, Index 14 (95% over 23bp)
GCCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTGC	655	0.11172098068847414	TruSeq Adapter, Index 14 (95% over 22bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	624	0.1064334228238288	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.4330680457527265	0.0
2	0.0	0.0	0.0	1.5422612326491347	0.0
3	0.0	0.0	0.0	2.449503822392637	0.0
4	0.0	0.0	0.0	2.9867879279936957	0.0
5	0.0	0.0	0.0	5.699646245322217	0.0
6	0.0	0.0	0.0	6.146871300841575	0.0
7	0.0	0.0	0.0	8.09218089588287	0.0
8	0.0	0.0	0.0	10.222896148952211	0.0
9	0.0	0.0	0.0	12.045739081193009	0.0
10	0.0	0.0	0.0	13.01745576360864	0.0
11	0.0	0.0	0.0	14.06592731825299	0.0
12	0.0	0.0	0.0	15.017176034740961	0.0
13	0.0	0.0	0.0	15.352850675954574	0.0
14	0.0	0.0	0.0	15.617228569186842	0.0
15	0.0	0.0	0.0	16.5611429312174	0.0
16	0.0	0.0	0.0	17.574989510167462	0.0
17	0.0	0.0	0.0	19.285770328954325	0.0
18	0.0	0.0	0.0	19.850345055792264	0.0
19	0.0	0.0	0.0	20.64842516058825	0.0
20	0.0	0.0	0.0	21.57221268945661	0.0
21	0.0	0.0	0.0	22.23759214848827	0.0
22	0.0	0.0	0.0	23.072855724719503	0.0
23	0.0	0.0	0.0	23.60570510436957	0.0
24	0.0	0.0	0.0	24.03672635352953	0.0
25	1.7056638273049487E-4	0.0	0.0	24.538532651522647	0.0
26	1.7056638273049487E-4	0.0	0.0	24.978423352584592	0.0
27	1.7056638273049487E-4	0.0	0.0	25.445092975735225	0.0
28	1.7056638273049487E-4	0.0	0.0	25.876114224895186	0.0
29	1.7056638273049487E-4	0.0	0.0	26.526483842246563	0.0
30	1.7056638273049487E-4	0.0	0.0	27.146151510706453	0.0
31	1.7056638273049487E-4	0.0	0.0	27.60821584152336	0.0
32	1.7056638273049487E-4	0.0	0.0	28.064480915327437	0.0
33	1.7056638273049487E-4	0.0	0.0	28.484244783227183	0.0
34	3.4113276546098975E-4	0.0	0.0	29.19925905963342	0.0
35	3.4113276546098975E-4	0.0	0.0	29.64136712367086	0.0
36	3.4113276546098975E-4	0.0	0.0	30.107695614056034	0.0
37	3.4113276546098975E-4	0.0	0.0	30.521319092177485	0.0
38	3.4113276546098975E-4	0.0	0.0	30.942106358373614	0.0
39	3.4113276546098975E-4	0.0	0.0	31.450735311675952	0.0
40	3.4113276546098975E-4	0.0	0.0	31.924398156518535	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTTACGG	35	1.0184158E-7	46.000004	1
ACGGGTC	35	1.0184158E-7	46.000004	4
GCGTAGG	105	0.0	46.000004	1
GCGAGAC	25	3.4150922E-5	46.0	20
CCTTCGC	20	6.308912E-4	46.0	15
TCGCCCG	20	6.308912E-4	46.0	43
CTTCGAA	20	6.308912E-4	46.0	12
CTATGCA	20	6.308912E-4	46.0	41
TAGAGGT	25	3.4150922E-5	46.0	38
ATCTCGG	20	6.308912E-4	46.0	1
AATCCAC	20	6.308912E-4	46.0	10
GCCTTCG	20	6.308912E-4	46.0	14
CAAACGG	30	1.8598312E-6	46.0	1
TGCCCGT	20	6.308912E-4	46.0	42
TGCCCAA	20	6.308912E-4	46.0	30
ACGCCCG	20	6.308912E-4	46.0	9
GTCGAGG	40	5.6024874E-9	46.0	1
ATCTACG	20	6.308912E-4	46.0	13
GTTTCGA	20	6.308912E-4	46.0	30
GAAGACG	25	3.4150922E-5	46.0	13
>>END_MODULE