FastQCFastQC Report
Thu 26 May 2016
SRR1544767_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544767_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences448764
Sequences flagged as poor quality0
Sequence length52
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTGCT39250.8746245242488256TruSeq Adapter, Index 14 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTGCTT31810.7088358246205132Illumina PCR Primer Index 8 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTGCT22790.5078393097485538TruSeq Adapter, Index 14 (95% over 23bp)
TGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTGCTTG15160.33781675892005597TruSeq Adapter, Index 14 (96% over 25bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA10930.24355786114750735No Hit
TCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTGCT9750.21726341685161912TruSeq Adapter, Index 14 (95% over 23bp)
ACCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTGC9340.2081272116301664No Hit
CGCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTGC8680.1934201495663645No Hit
GCCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTGC7860.1751477391234591TruSeq Adapter, Index 14 (95% over 22bp)
AGCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTGC6310.14060842670089402No Hit
ACTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTGCT6000.133700564216381Illumina PCR Primer Index 8 (95% over 21bp)
CGTTCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCT4990.11119430257329019No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATGCGG750.046.0000041
TGTCACA206.3073216E-446.023
CGGGTAT501.6370905E-1146.05
CGTGGGA253.4138036E-546.03
GATACGT253.4138036E-546.035
ATCTCGG301.8588489E-646.01
CCCCTGA206.3073216E-446.039
ACCGGTC253.4138036E-546.030
ACCGGTA301.8588489E-646.041
ACCGGGT301.8588489E-646.03
TCCGCGC206.3073216E-446.043
CGTGCCC405.5988494E-946.039
GGCACGG253.4138036E-546.01
GACCGGT253.4138036E-546.029
GACCGAG206.3073216E-446.023
GATCCCA253.4138036E-546.08
ATAGCGG800.046.01
CAACCGG253.4138036E-546.01
CGACCGA206.3073216E-446.022
CGACCCT206.3073216E-446.07
Produced by FastQC (version 0.11.5)