FastQCFastQC Report
Thu 26 May 2016
SRR1544766_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544766_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1519983
Sequences flagged as poor quality0
Sequence length52
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTCTGCT56720.37316206825997394No Hit
CTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTCTGCTT40670.2675687820192726Illumina Single End Adapter 2 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTCTGCT37710.24809488000852642No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28740.189081062090826No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC22050.14506741193815983No Hit
ACCTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTCTGC16840.11079071279086673No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA16400.10789594357305313No Hit
CGCTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTCTGC15740.1035537897463327No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCATA206.3120987E-446.00000414
ACGGGTA405.6134013E-946.0000044
CGCTAGC206.3120987E-446.00000446
AACGCCT206.3120987E-446.00000416
GCGATCG206.3120987E-446.0000048
GTACGAC206.3120987E-446.00000429
ACGTTAT253.4176795E-546.09
CTAACGG1900.046.01
GTGTCGA301.861803E-646.030
CCAACGA253.4176795E-546.028
CGTGACG351.0198528E-745.99999618
CGTAGGG6750.043.955562
TTCACGG1050.043.8095251
TTACGGG5400.043.4444432
CATGCGG3300.043.212121
TAACGGG3900.043.0512852
CGTTTTT20650.042.9927371
GTCGAGG1450.042.8275831
AATGCGG3200.042.4062541
TACGGGT1250.042.323