##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544766_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1519983
Sequences flagged as poor quality	0
Sequence length	52
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.909076614672664	34.0	33.0	34.0	31.0	34.0
2	33.248541595531	34.0	33.0	34.0	31.0	34.0
3	33.314444306285004	34.0	34.0	34.0	31.0	34.0
4	36.59431519957789	37.0	37.0	37.0	35.0	37.0
5	36.5884552656181	37.0	37.0	37.0	35.0	37.0
6	36.684506997775635	37.0	37.0	37.0	35.0	37.0
7	36.66966604231758	37.0	37.0	37.0	35.0	37.0
8	36.57938147992444	37.0	37.0	37.0	35.0	37.0
9	38.35536581659137	39.0	39.0	39.0	37.0	39.0
10	38.173306543560024	39.0	39.0	39.0	35.0	39.0
11	37.620801022116694	39.0	35.0	39.0	35.0	39.0
12	37.56025100280727	39.0	35.0	39.0	35.0	39.0
13	37.54596729042364	39.0	35.0	39.0	35.0	39.0
14	38.88180986234715	41.0	36.0	41.0	35.0	41.0
15	38.91179440822693	41.0	36.0	41.0	35.0	41.0
16	38.88121248724492	41.0	36.0	41.0	35.0	41.0
17	38.92667812732116	41.0	36.0	41.0	35.0	41.0
18	38.921202408184826	41.0	36.0	41.0	35.0	41.0
19	38.865650471090795	41.0	36.0	41.0	35.0	41.0
20	38.789188431712724	41.0	35.0	41.0	35.0	41.0
21	38.73101804428076	41.0	35.0	41.0	35.0	41.0
22	38.71273165555141	41.0	35.0	41.0	35.0	41.0
23	38.67163514328779	41.0	35.0	41.0	35.0	41.0
24	38.619412190794236	41.0	35.0	41.0	35.0	41.0
25	38.5823624343167	41.0	35.0	41.0	35.0	41.0
26	38.513726798260244	40.0	35.0	41.0	35.0	41.0
27	38.483967912799024	40.0	36.0	41.0	35.0	41.0
28	38.52122359263229	40.0	36.0	41.0	35.0	41.0
29	38.51288928889336	40.0	36.0	41.0	35.0	41.0
30	38.454202448316856	40.0	35.0	41.0	35.0	41.0
31	38.40643546671246	40.0	35.0	41.0	35.0	41.0
32	38.32344638065031	40.0	35.0	41.0	35.0	41.0
33	38.24827975049721	40.0	35.0	41.0	35.0	41.0
34	38.159084673973325	40.0	35.0	41.0	35.0	41.0
35	38.09461684768843	40.0	35.0	41.0	35.0	41.0
36	38.024916068140236	40.0	35.0	41.0	35.0	41.0
37	37.98104781435055	40.0	35.0	41.0	35.0	41.0
38	37.92506034606966	40.0	35.0	41.0	35.0	41.0
39	37.81812822906572	40.0	35.0	41.0	35.0	41.0
40	37.73066146134529	40.0	35.0	41.0	34.0	41.0
41	37.69186563270773	40.0	35.0	41.0	34.0	41.0
42	37.60985089964822	40.0	35.0	41.0	34.0	41.0
43	37.606519941341446	40.0	35.0	41.0	34.0	41.0
44	37.56427473202003	40.0	35.0	41.0	34.0	41.0
45	37.52635457107086	39.0	35.0	41.0	34.0	41.0
46	37.471797381944405	39.0	35.0	41.0	34.0	41.0
47	37.36586264451642	39.0	35.0	41.0	34.0	41.0
48	37.317693684732	39.0	35.0	41.0	34.0	41.0
49	37.241110591368454	39.0	35.0	41.0	34.0	41.0
50	37.08708913191792	39.0	35.0	41.0	34.0	41.0
51	36.944675697030824	38.0	35.0	41.0	34.0	41.0
52	36.65749419565877	37.0	35.0	40.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	2.0
14	7.0
15	16.0
16	23.0
17	39.0
18	88.0
19	191.0
20	361.0
21	639.0
22	1003.0
23	1611.0
24	2739.0
25	4239.0
26	6149.0
27	7308.0
28	7891.0
29	8678.0
30	10229.0
31	12641.0
32	16224.0
33	22520.0
34	72213.0
35	262037.0
36	56192.0
37	86398.0
38	161930.0
39	771170.0
40	7444.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.391217533353995	22.536633633402477	24.529945400705138	20.54220343253839
2	26.447532636878172	24.019873906484477	27.20537006005988	22.327223396577462
3	26.308254763375643	24.72264492431823	26.00002763188799	22.969072680418137
4	26.942998704590774	26.891287599927104	22.967427925180743	23.19828577030138
5	25.204623999084202	35.383619422059326	20.112067042855085	19.299689536001388
6	88.75987428806769	2.637792659523166	6.223951188927771	2.378381863481368
7	90.28739137214035	2.1189052772300743	4.497681881968417	3.0960214686611627
8	81.129131049492	4.406957183073758	8.803519513047185	5.660392254387055
9	45.5587332226742	20.57549327854325	12.652641509806358	21.21313198897619
10	36.80087211501707	20.130554091723393	25.67147132566614	17.39710246759339
11	35.50783133758733	20.093711574405766	24.263166101199815	20.13529098680709
12	21.80149383249681	30.87935851914133	27.20958063346761	20.109567014894246
13	17.200652902039035	33.45083464749277	29.258287757165707	20.090224693302492
14	16.825517127494187	25.343572921539252	38.14161079433126	19.689299156635304
15	19.04606827839522	21.06280136027837	40.39446493809471	19.496665423231708
16	19.589890150087204	22.375776571185334	27.195106787378542	30.839226491348914
17	20.978524101914296	24.853699021633794	30.381392423467897	23.786384452984013
18	24.094611584471668	27.84564037887266	26.91786684456339	21.141881192092278
19	28.069063930320276	24.030202969375317	25.025016727160764	22.875716373143646
20	23.05617891779053	28.14847271318166	27.51945252019266	21.27589584883515
21	21.94965338428127	24.012176451973477	27.6757042677451	26.362465896000153
22	20.137659434348937	28.00215528726308	25.98035635924876	25.879828919139225
23	21.464779540297492	22.463738081281175	34.8621662215959	21.209316156825437
24	21.501029945729655	23.222364986976828	31.490681145775973	23.785923921517544
25	19.505547101513635	30.038033320109502	27.30885805959672	23.147561518780144
26	19.038041872836736	28.514529438816094	27.75109984782724	24.696328840519925
27	20.343122258604208	26.33549191010689	32.436218036649095	20.88516779463981
28	18.768762545370574	25.396534040183344	30.92508271474089	24.909620699705194
29	23.029731253573228	25.58482561976022	28.682426053449284	22.703017073217264
30	26.15871361719177	24.844948923770858	23.86862221485372	25.12771524418365
31	28.085774643532197	26.046607100210988	24.85310690974833	21.01451134650848
32	25.255677201652915	26.284965029214142	26.250556749647856	22.208801019485087
33	20.092658931053833	26.522138734446372	26.889313893642232	26.495888440857563
34	20.673323320063446	24.88435725925882	31.096992532153322	23.34532688852441
35	25.522653871786723	25.856341814349236	26.361347462438722	22.259656851425312
36	22.301104683407644	29.43500025987133	26.457401168302543	21.806493888418487
37	21.86103397209048	28.702557857554982	26.98017017295588	22.456237997398656
38	20.93253674547676	27.910904266692455	26.50759909814781	24.648959889682974
39	24.893567888588226	25.280348530213825	26.623653027698335	23.20243055349961
40	18.384613512124805	23.720002131602786	30.404287416372423	27.491096939899982
41	21.72603246220517	26.783194285725564	24.265337178113175	27.22543607395609
42	22.487883088166118	23.93704403272931	27.505833946827035	26.069238932277532
43	22.938085491745632	26.036212247110658	27.415569779398847	23.610132481744863
44	20.2485159373493	28.22985520232792	25.71509023456183	25.806538625760943
45	21.77866462980178	26.64082427237673	26.49253314017328	25.08797795764821
46	22.917361575754466	24.97929253156121	27.692414980957025	24.410930911727302
47	21.763664462036747	22.64722697556486	31.81969798346429	23.769410578934107
48	20.676481250119245	22.18261651610577	29.352696707792127	27.788205525982857
49	19.948578372258112	25.67633980116883	30.379352926973525	23.995728899599538
50	19.399164332758982	26.387466175608544	28.470581578872924	25.742787912759553
51	20.800890536275734	23.358879671680537	29.29986716956703	26.5403626224767
52	22.50597539577745	21.90406076910071	31.644761816415052	23.945202018706787
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	236.0
1	529.5
2	823.0
3	3230.5
4	5638.0
5	3773.5
6	1909.0
7	2040.5
8	2172.0
9	2078.5
10	1985.0
11	1962.0
12	1939.0
13	1871.0
14	1863.0
15	1923.0
16	1831.5
17	1740.0
18	1895.5
19	2051.0
20	2363.0
21	2675.0
22	3313.5
23	3952.0
24	4388.0
25	4824.0
26	6715.0
27	8606.0
28	10272.5
29	11939.0
30	14454.5
31	16970.0
32	20364.0
33	23758.0
34	27988.0
35	32218.0
36	34786.0
37	37354.0
38	39774.5
39	46902.5
40	51610.0
41	66109.0
42	80608.0
43	95893.5
44	111179.0
45	122550.5
46	133922.0
47	142067.0
48	150212.0
49	150202.5
50	150193.0
51	136662.0
52	123131.0
53	111594.0
54	100057.0
55	90427.0
56	80797.0
57	74675.0
58	68553.0
59	67796.5
60	67040.0
61	64555.5
62	62071.0
63	55210.0
64	39838.0
65	31327.0
66	25211.0
67	19095.0
68	15720.5
69	12346.0
70	10476.0
71	8606.0
72	7302.5
73	5999.0
74	5055.5
75	4112.0
76	3277.0
77	2442.0
78	1725.0
79	1008.0
80	679.5
81	351.0
82	284.0
83	217.0
84	122.5
85	28.0
86	17.5
87	7.0
88	6.5
89	4.5
90	3.0
91	2.5
92	2.0
93	1.5
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1519983.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.250268909336757
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.04704398335504	14.885185209504339
2	12.340071398818186	5.244596711706036
3	4.754631217053162	3.0311157578132035
4	2.351548677957966	1.9988416704000846
5	1.306495958624952	1.388169522487097
6	0.7818127896159743	0.9968239209658907
7	0.5331649986601786	0.7930929716202477
8	0.3901514892955432	0.6632659250326812
9	0.3175012420995435	0.6072288096297359
>10	4.587757538076788	25.900531495630958
>50	2.3585873245416953	34.17473411166646
>100	0.22037006865790162	7.347374616457825
>500	0.0065179879459124745	0.9381016469618256
>1k	0.0040349449188981975	1.6568314259777162
>5k	3.1038037837678446E-4	0.3741062041459238
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTCTGCT	5672	0.37316206825997394	No Hit
CTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTCTGCTT	4067	0.2675687820192726	Illumina Single End Adapter 2 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTCTGCT	3771	0.24809488000852642	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2874	0.189081062090826	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	2205	0.14506741193815983	No Hit
ACCTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTCTGC	1684	0.11079071279086673	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA	1640	0.10789594357305313	No Hit
CGCTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTCTGC	1574	0.1035537897463327	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.2744767540163278	0.0
2	0.0	0.0	0.0	1.0767225686076751	0.0
3	0.0	0.0	0.0	1.740808943257918	0.0
4	0.0	0.0	0.0	2.112589417118481	0.0
5	0.0	0.0	0.0	4.217481379725958	0.0
6	0.0	0.0	0.0	4.502550357471104	0.0
7	0.0	0.0	0.0	5.468284842659425	0.0
8	6.57902094957641E-5	0.0	0.0	6.675140445649721	0.0
9	6.57902094957641E-5	0.0	0.0	7.420543519236729	0.0
10	6.57902094957641E-5	0.0	0.0	8.20469702621674	0.0
11	6.57902094957641E-5	0.0	0.0	8.90595486923209	0.0
12	6.57902094957641E-5	0.0	0.0	9.4789218037307	0.0
13	1.315804189915282E-4	0.0	0.0	9.747740599730392	0.0
14	1.315804189915282E-4	0.0	0.0	9.95386132608062	0.0
15	1.315804189915282E-4	0.0	0.0	10.667948259947645	0.0
16	1.315804189915282E-4	0.0	0.0	11.460193962695636	0.0
17	1.9737062848729228E-4	0.0	0.0	12.403296615817414	0.0
18	1.9737062848729228E-4	0.0	0.0	12.918499746378743	0.0
19	1.9737062848729228E-4	0.0	0.0	13.431597590236207	0.0
20	1.9737062848729228E-4	0.0	0.0	14.145026622008272	0.0
21	1.9737062848729228E-4	0.0	0.0	14.782336381393739	0.0
22	1.9737062848729228E-4	0.0	0.0	15.488331119492784	0.0
23	1.9737062848729228E-4	0.0	0.0	16.03991623590527	0.0
24	2.631608379830564E-4	0.0	0.0	16.505776709344776	0.0
25	3.289510474788205E-4	0.0	0.0	16.9573607073237	0.0
26	3.289510474788205E-4	0.0	0.0	17.36769424394878	0.0
27	3.289510474788205E-4	0.0	0.0	17.79967275949797	0.0
28	3.289510474788205E-4	0.0	0.0	18.24369088338488	0.0
29	3.289510474788205E-4	0.0	0.0	18.757841370594278	0.0
30	3.289510474788205E-4	0.0	0.0	19.23942570410327	0.0
31	3.289510474788205E-4	0.0	0.0	19.676404275574136	0.0
32	3.289510474788205E-4	0.0	0.0	20.111474930969624	0.0
33	3.289510474788205E-4	0.0	0.0	20.541808691281414	0.0
34	3.9474125697458456E-4	0.0	0.0	21.077538367205424	0.0
35	3.9474125697458456E-4	0.0	0.0	21.51635906454217	0.0
36	3.9474125697458456E-4	0.0	0.0	21.98057478274428	0.0
37	3.9474125697458456E-4	0.0	0.0	22.419000738824053	0.0
38	3.9474125697458456E-4	0.0	0.0	22.86663732423323	0.0
39	3.9474125697458456E-4	0.0	0.0	23.35762965770012	0.0
40	3.9474125697458456E-4	0.0	0.0	23.86178003306616	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCATA	20	6.3120987E-4	46.000004	14
ACGGGTA	40	5.6134013E-9	46.000004	4
CGCTAGC	20	6.3120987E-4	46.000004	46
AACGCCT	20	6.3120987E-4	46.000004	16
GCGATCG	20	6.3120987E-4	46.000004	8
GTACGAC	20	6.3120987E-4	46.000004	29
ACGTTAT	25	3.4176795E-5	46.0	9
CTAACGG	190	0.0	46.0	1
GTGTCGA	30	1.861803E-6	46.0	30
CCAACGA	25	3.4176795E-5	46.0	28
CGTGACG	35	1.0198528E-7	45.999996	18
CGTAGGG	675	0.0	43.95556	2
TTCACGG	105	0.0	43.809525	1
TTACGGG	540	0.0	43.444443	2
CATGCGG	330	0.0	43.21212	1
TAACGGG	390	0.0	43.051285	2
CGTTTTT	2065	0.0	42.992737	1
GTCGAGG	145	0.0	42.827583	1
AATGCGG	320	0.0	42.406254	1
TACGGGT	125	0.0	42.32	3
>>END_MODULE