FastQCFastQC Report
Thu 26 May 2016
SRR1544765_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544765_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1070544
Sequences flagged as poor quality0
Sequence length52
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTCTGCT66780.623795005156257No Hit
CTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTCTGCTT46440.4337981437474779Illumina Single End Adapter 2 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTCTGCT40190.3754166106203949No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA34480.32207924195549176No Hit
TGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTCTGCTTG23400.21858046002779896Illumina Single End Adapter 2 (95% over 22bp)
ACCTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTCTGC16050.1499237770703493No Hit
CGCTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTCTGC15540.1451598439671793No Hit
TCTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTCTGCT15020.140302500411006No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC14540.135818798666846No Hit
GCCTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTCTGC12960.12105994709231943No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12930.12077971573330942No Hit
AGCTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTCTGC11350.10602086415878283No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATACG351.019489E-746.0000049
CGAAACG253.416997E-546.034
CCTGTCG206.311259E-446.039
ACGGGTA301.8612827E-646.04
TCATCGG206.311259E-446.01
ATAACGG405.6115823E-946.01
CGGTAAG206.311259E-446.030
CAGACGA206.311259E-446.041
TACGGGA2400.044.0833323
TTACGGG3250.043.8769232
GTCCCCC4200.043.8095259
TCGAAGG1050.043.8095251
CGTAGGG3950.043.6708832
TACGGGT750.042.933333
CGAATAT1450.042.82758314
ATTGCGG2100.042.7142871
TAACGGG2600.042.461542
CGAGGGT1300.042.461543
ATAGCGG1950.042.4615361
TATGCGG1150.042.01