##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544765_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1070544
Sequences flagged as poor quality	0
Sequence length	52
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.94190150054552	34.0	33.0	34.0	31.0	34.0
2	33.28842906036557	34.0	34.0	34.0	31.0	34.0
3	33.386353106458024	34.0	34.0	34.0	31.0	34.0
4	36.614845349654004	37.0	37.0	37.0	35.0	37.0
5	36.6129883498483	37.0	37.0	37.0	35.0	37.0
6	36.557962120193096	37.0	37.0	37.0	35.0	37.0
7	36.464378857851706	37.0	37.0	37.0	35.0	37.0
8	36.486127613624475	37.0	37.0	37.0	35.0	37.0
9	38.31373114977058	39.0	39.0	39.0	37.0	39.0
10	38.19808433842981	39.0	39.0	39.0	35.0	39.0
11	37.7048892899311	39.0	37.0	39.0	35.0	39.0
12	37.68681156496137	39.0	37.0	39.0	35.0	39.0
13	37.672666420063074	39.0	37.0	39.0	35.0	39.0
14	38.99132590533411	41.0	38.0	41.0	35.0	41.0
15	39.016134787547266	41.0	38.0	41.0	35.0	41.0
16	39.00127692089256	41.0	38.0	41.0	35.0	41.0
17	39.05278998341031	41.0	37.0	41.0	35.0	41.0
18	39.06198250609036	41.0	37.0	41.0	35.0	41.0
19	39.01709598110867	41.0	37.0	41.0	35.0	41.0
20	38.95332746715688	41.0	37.0	41.0	35.0	41.0
21	38.89714014557085	41.0	36.0	41.0	35.0	41.0
22	38.88567588067375	41.0	36.0	41.0	35.0	41.0
23	38.85592651960125	41.0	36.0	41.0	35.0	41.0
24	38.78753885874845	41.0	36.0	41.0	35.0	41.0
25	38.736895447548164	41.0	36.0	41.0	35.0	41.0
26	38.671113004229625	41.0	35.0	41.0	35.0	41.0
27	38.646457315159395	41.0	36.0	41.0	35.0	41.0
28	38.698810137649645	41.0	36.0	41.0	35.0	41.0
29	38.729184414652735	41.0	36.0	41.0	35.0	41.0
30	38.69424703702043	41.0	36.0	41.0	35.0	41.0
31	38.676512128413215	41.0	35.0	41.0	35.0	41.0
32	38.62837585377154	41.0	36.0	41.0	35.0	41.0
33	38.56639241357665	41.0	36.0	41.0	35.0	41.0
34	38.50524219462255	41.0	35.0	41.0	35.0	41.0
35	38.43269122987939	40.0	35.0	41.0	35.0	41.0
36	38.40339677771301	40.0	35.0	41.0	35.0	41.0
37	38.36239239115814	40.0	35.0	41.0	35.0	41.0
38	38.293100517120266	40.0	35.0	41.0	35.0	41.0
39	38.243177300512635	40.0	35.0	41.0	35.0	41.0
40	38.1748942593672	40.0	35.0	41.0	35.0	41.0
41	38.143879186656505	40.0	35.0	41.0	35.0	41.0
42	38.08610575557847	40.0	35.0	41.0	35.0	41.0
43	38.010136902359925	40.0	35.0	41.0	35.0	41.0
44	37.86899837839454	40.0	35.0	41.0	35.0	41.0
45	37.828871115993365	40.0	35.0	41.0	35.0	41.0
46	37.76377337129534	40.0	35.0	41.0	35.0	41.0
47	37.66829761317611	39.0	35.0	41.0	35.0	41.0
48	37.58668957090974	39.0	35.0	41.0	35.0	41.0
49	37.51443378319807	39.0	35.0	41.0	35.0	41.0
50	37.32793887967239	39.0	35.0	41.0	35.0	41.0
51	37.153759210270664	39.0	35.0	40.0	34.0	41.0
52	36.72199274387601	37.0	35.0	40.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	0.0
13	4.0
14	7.0
15	8.0
16	30.0
17	44.0
18	73.0
19	140.0
20	209.0
21	350.0
22	566.0
23	900.0
24	1481.0
25	2334.0
26	3352.0
27	4112.0
28	4342.0
29	4603.0
30	5652.0
31	7131.0
32	9369.0
33	13394.0
34	44731.0
35	177254.0
36	36990.0
37	59091.0
38	116151.0
39	574871.0
40	3353.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.463536295565618	22.30921849078599	23.742228250310124	22.485016963338264
2	25.955588934224096	24.50333662138128	27.050359443423154	22.490715000971466
3	26.458604223647043	24.64662825628839	25.75924016201109	23.135527358053476
4	26.937052563930113	26.384623144868403	23.260043491906917	23.418280799294564
5	25.08612443766907	34.49321092827572	20.2214014557085	20.199263178346712
6	86.66575124422722	2.6675223064161773	7.394091228384821	3.2726352209717673
7	88.47445784573077	2.150028396777713	5.333736866490308	4.041776891001211
8	77.98138142850738	4.561792882870765	10.4932632381294	6.96356245049246
9	44.33278781628779	19.72735356977387	13.642036198418747	22.297822415519587
10	35.23414264149815	19.694006038051683	27.4299795244287	17.641871796021462
11	33.78347830635639	19.71810593492654	24.933958809726644	21.564456948990422
12	21.326633935643933	29.36236156570865	28.502424935359965	20.80857956328745
13	16.96165687725119	32.14094890074579	30.190632052489203	20.70676216951382
14	16.59333946105905	25.389428178570896	37.215378349698845	20.80185401067121
15	18.531699771331212	20.931227488080825	40.13034494612085	20.406727794467113
16	18.910198926900716	23.143373836105756	27.203832817707635	30.742594419285897
17	20.024865862589486	24.8907097699861	30.48151220314158	24.60291216428283
18	23.25920279782989	27.873118713476515	27.447073637328312	21.420604851365287
19	26.674756011896754	23.875992019010894	25.364954639884026	24.08429732920833
20	22.052152924120822	28.65692582462748	27.792412082081636	21.498509169170067
21	21.43723191199988	23.87309629496779	27.843133958062445	26.846537834969887
22	19.739216697305295	27.725156555919234	25.83817199479891	26.69745475197656
23	20.656694166704032	22.42065716121897	35.02873305534383	21.893915616733175
24	21.997320988207864	23.067898190079063	30.681223751662706	24.253557070050366
25	19.401724730604254	29.60784423620141	26.96021835627494	24.030212676919398
26	19.37248725881421	27.442776756490154	27.270060828886994	25.914675155808638
27	21.493745236066896	25.461541048289465	31.3957203066852	21.648993408958436
28	19.25591101346605	24.7337802089405	29.86388228788354	26.146426489709906
29	23.9517478963966	24.668392891838167	28.0352792598903	23.344579951874938
30	25.359723654515836	24.422536579533396	23.43696288989523	26.78077687605554
31	28.372117353420318	25.10433947600472	24.338186940471388	22.185356230103572
32	24.571619662526714	25.78950514878417	26.677931967298868	22.960943221390245
33	19.888766832563633	25.804544231717706	26.38098013720127	27.92570879851739
34	21.07367842891091	23.671983589651617	31.69902404758702	23.555313933850453
35	24.924524353973307	25.725705809382895	26.400783153237978	22.94898668340582
36	22.442982259486765	29.00983051607407	25.94083008264957	22.606357141789594
37	21.642548087701204	28.0101518480324	27.894976759479295	22.4523233047871
38	20.61148350745042	27.681627284819683	26.004909653409857	25.701979554320047
39	25.315914152057275	24.666618093231104	26.747055702521333	23.270412052190288
40	18.36636326951531	22.949173504311826	30.698878327280337	27.985584898892522
41	21.59985951067868	26.74518749346127	23.986683405819846	27.668269590040207
42	22.03561927393923	23.070513682763156	28.01173982573346	26.882127217564154
43	23.608931533874365	25.011489485719412	27.19421154104829	24.185367439357933
44	20.430080407717945	26.528755473852545	26.394711473792764	26.64645264463675
45	21.417989358681194	25.293028591071455	26.703900073233793	26.58508197701356
46	23.40968703761826	23.647883692776755	27.831551061890032	25.110878207714954
47	20.813530317296628	21.974902479487067	32.8054708634115	24.406096339804808
48	20.401777040457937	21.085074504177314	29.238592715479232	29.274555739885518
49	19.94135691760451	23.969962934732248	31.172842965819246	24.915837181843997
50	19.483085235170154	24.591329268110417	29.500702446606585	26.42488305011284
51	20.76178092633278	22.014975563825494	29.24541167854848	27.977831831293248
52	21.90717990105965	21.155599396194834	33.166875906081394	23.770344796664126
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	161.0
1	309.5
2	458.0
3	1596.0
4	2734.0
5	1897.0
6	1060.0
7	1093.0
8	1126.0
9	1114.5
10	1103.0
11	1132.0
12	1161.0
13	1108.5
14	1122.0
15	1188.0
16	1093.5
17	999.0
18	1171.5
19	1344.0
20	1553.0
21	1762.0
22	2082.0
23	2402.0
24	2716.5
25	3031.0
26	4187.5
27	5344.0
28	6451.0
29	7558.0
30	9085.0
31	10612.0
32	12797.0
33	14982.0
34	17548.0
35	20114.0
36	21689.5
37	23265.0
38	25139.5
39	30019.0
40	33024.0
41	42258.0
42	51492.0
43	62825.0
44	74158.0
45	83524.5
46	92891.0
47	104509.0
48	116127.0
49	118066.0
50	120005.0
51	107510.5
52	95016.0
53	84487.5
54	73959.0
55	65727.0
56	57495.0
57	52495.0
58	47495.0
59	46678.5
60	45862.0
61	43472.5
62	41083.0
63	38147.5
64	28221.0
65	21230.0
66	17174.5
67	13119.0
68	10676.5
69	8234.0
70	7120.5
71	6007.0
72	5063.0
73	4119.0
74	3443.5
75	2768.0
76	2232.5
77	1697.0
78	1184.5
79	672.0
80	459.0
81	246.0
82	191.0
83	136.0
84	75.5
85	15.0
86	9.0
87	3.0
88	1.5
89	2.5
90	5.0
91	2.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1070544.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.85228973084415
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.12195796628372	17.426912158721578
2	12.140365883734713	6.034317807620618
3	4.515002929714839	3.366244828346501
4	2.119851303091389	2.1073263508293882
5	1.230490361118905	1.5290251482769035
6	0.756901591882271	1.128644259551721
7	0.548859932070078	0.9548298237419912
8	0.4299609260233945	0.854841080518036
9	0.3401272579067718	0.760764704297098
>10	7.025754194467518	46.91201794845311
>50	0.6480003308791874	9.910145680897713
>100	0.11064340181038791	4.795155675322946
>500	0.007552450635521259	1.3134944482203628
>1k	0.004153847849536692	2.27956417171139
>5k	3.77622531776063E-4	0.6267159134906819
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTCTGCT	6678	0.623795005156257	No Hit
CTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTCTGCTT	4644	0.4337981437474779	Illumina Single End Adapter 2 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTCTGCT	4019	0.3754166106203949	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA	3448	0.32207924195549176	No Hit
TGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTCTGCTTG	2340	0.21858046002779896	Illumina Single End Adapter 2 (95% over 22bp)
ACCTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTCTGC	1605	0.1499237770703493	No Hit
CGCTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTCTGC	1554	0.1451598439671793	No Hit
TCTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTCTGCT	1502	0.140302500411006	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	1454	0.135818798666846	No Hit
GCCTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTCTGC	1296	0.12105994709231943	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1293	0.12077971573330942	No Hit
AGCTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTCTGC	1135	0.10602086415878283	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.4442601144838512	0.0
2	0.0	0.0	0.0	1.6969876997115485	0.0
3	0.0	0.0	0.0	2.6515491189526075	0.0
4	0.0	0.0	0.0	3.2043521798263312	0.0
5	0.0	0.0	0.0	6.164903077313964	0.0
6	0.0	0.0	0.0	6.574414503280575	0.0
7	0.0	0.0	0.0	7.9540868941398015	0.0
8	0.0	0.0	0.0	9.578868313679774	0.0
9	0.0	0.0	0.0	10.611147229819606	0.0
10	0.0	0.0	0.0	11.704142940411604	0.0
11	0.0	0.0	0.0	12.63226920145272	0.0
12	0.0	0.0	0.0	13.428593313306132	0.0
13	0.0	0.0	0.0	13.795322751797217	0.0
14	0.0	0.0	0.0	14.062009595121733	0.0
15	1.8682090600666577E-4	0.0	0.0	14.993685453376974	0.0
16	1.8682090600666577E-4	0.0	0.0	16.015782630139444	0.0
17	1.8682090600666577E-4	0.0	0.0	17.125965864084055	0.0
18	1.8682090600666577E-4	0.0	0.0	17.757980989104606	0.0
19	1.8682090600666577E-4	0.0	0.0	18.386633337817035	0.0
20	1.8682090600666577E-4	0.0	0.0	19.24834476677278	0.0
21	2.8023135900999863E-4	0.0	0.0	19.98965012180723	0.0
22	2.8023135900999863E-4	0.0	0.0	20.821563616254913	0.0
23	2.8023135900999863E-4	0.0	0.0	21.44451792733414	0.0
24	2.8023135900999863E-4	0.0	0.0	21.984150114334394	0.0
25	2.8023135900999863E-4	0.0	0.0	22.505660673452002	0.0
26	2.8023135900999863E-4	0.0	0.0	22.980839647879957	0.0
27	2.8023135900999863E-4	0.0	0.0	23.46573330942026	0.0
28	2.8023135900999863E-4	0.0	0.0	23.961929635773963	0.0
29	2.8023135900999863E-4	0.0	0.0	24.51632067434874	0.0
30	2.8023135900999863E-4	0.0	0.0	25.0616508989822	0.0
31	2.8023135900999863E-4	0.0	0.0	25.536362821145136	0.0
32	2.8023135900999863E-4	0.0	0.0	26.013596825539164	0.0
33	2.8023135900999863E-4	0.0	0.0	26.488402158155107	0.0
34	2.8023135900999863E-4	0.0	0.0	27.050266032970153	0.0
35	2.8023135900999863E-4	0.0	0.0	27.51348846941368	0.0
36	2.8023135900999863E-4	0.0	0.0	28.006882482177286	0.0
37	2.8023135900999863E-4	0.0	0.0	28.464313470534606	0.0
38	2.8023135900999863E-4	0.0	0.0	28.94360250489471	0.0
39	2.8023135900999863E-4	0.0	0.0	29.458480921849077	0.0
40	2.8023135900999863E-4	0.0	0.0	29.973265928350447	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGATACG	35	1.019489E-7	46.000004	9
CGAAACG	25	3.416997E-5	46.0	34
CCTGTCG	20	6.311259E-4	46.0	39
ACGGGTA	30	1.8612827E-6	46.0	4
TCATCGG	20	6.311259E-4	46.0	1
ATAACGG	40	5.6115823E-9	46.0	1
CGGTAAG	20	6.311259E-4	46.0	30
CAGACGA	20	6.311259E-4	46.0	41
TACGGGA	240	0.0	44.083332	3
TTACGGG	325	0.0	43.876923	2
GTCCCCC	420	0.0	43.809525	9
TCGAAGG	105	0.0	43.809525	1
CGTAGGG	395	0.0	43.670883	2
TACGGGT	75	0.0	42.93333	3
CGAATAT	145	0.0	42.827583	14
ATTGCGG	210	0.0	42.714287	1
TAACGGG	260	0.0	42.46154	2
CGAGGGT	130	0.0	42.46154	3
ATAGCGG	195	0.0	42.461536	1
TATGCGG	115	0.0	42.0	1
>>END_MODULE