FastQCFastQC Report
Thu 26 May 2016
SRR1544764_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544764_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences780953
Sequences flagged as poor quality0
Sequence length52
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTGCT39760.5091215476475537No Hit
CTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTGCTT35580.45559719983148794Illumina Single End Adapter 2 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTGCT28640.3667314166153405No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17550.22472543162008468No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC11590.14840841894454596No Hit
CGCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTGC11260.14418281253801443No Hit
ACCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTGC10620.13598769708292305No Hit
GCCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTGC9770.12510355936912976No Hit
TCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTGCT8740.11191454543359204No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA8030.1028230892256No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGGTT301.8606315E-646.013
AGCAAGT206.3102064E-446.028
CGACGGT206.3102064E-446.027
GCGAAAA206.3102064E-446.06
TAGGTTG301.8606315E-646.030
TTCACGG405.6079443E-946.01
TAAACGG253.4161432E-546.01
ATTCGGT206.3102064E-446.08
TAACGTG206.3102064E-446.044
TCTAGTA206.3102064E-446.023
TCTAGAT301.8606315E-646.011
AATGCGG650.046.01
CTAACGT206.3102064E-446.043
TACGCGG850.046.01
GTTCCGA206.3102064E-446.045
TACTCGC253.4161432E-546.044
CGTCGGA206.3102064E-446.041
ATCGGTC253.4161432E-546.025
GACGATT206.3102064E-446.014
CCACGAA253.4161432E-546.041
Produced by FastQC (version 0.11.5)