FastQCFastQC Report
Thu 26 May 2016
SRR1544763_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544763_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences602393
Sequences flagged as poor quality0
Sequence length52
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTGCT55340.9186693736481002No Hit
CTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTGCTT41750.6930691425697179Illumina Single End Adapter 2 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTGCT31140.5169382778352338No Hit
TGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTGCTTG21780.3615579862315797Illumina Single End Adapter 2 (95% over 22bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA18310.30395439522039597No Hit
TCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTGCT11620.1928973278241945No Hit
ACCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTGC11490.1907392682185882No Hit
CGCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTGC11130.1847631031569092No Hit
GCCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTGC11110.18443109398681592No Hit
AGCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTGC8860.14708006235132215No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC8360.1387798330989902No Hit
ACTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTGCT7920.13147563135693807No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7120.11819526455320696No Hit
CGTTCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCT6730.11172108573638805No Hit
GGCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTGC6140.10192681521863634No Hit
CTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTGCTTGAAA6070.10076478312330986Illumina Single End Adapter 2 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCACGA301.8599167E-646.023
AGTTTCG206.309051E-446.012
CAAGTAA206.309051E-446.013
CGACGGT301.8599167E-646.027
CATGCGG1350.046.01
CGTGAAC253.4152054E-546.033
TGAACGG301.8599167E-646.01
GTTAGCG206.309051E-446.010
CCTCGCT206.309051E-446.026
TCAGCGA301.8599167E-646.025
AACATCG206.309051E-446.037
ACTACGG301.8599167E-646.01
TCCACGG206.309051E-446.01
CGGGATA405.6043064E-946.05
AAATTAG206.309051E-446.033
GCGATGT453.092282E-1046.08
ATGACCA206.309051E-446.043
TACTCGC206.309051E-446.044
CCGACGG453.092282E-1046.01
ATGCGCA206.309051E-446.011
Produced by FastQC (version 0.11.5)