FastQCFastQC Report
Thu 26 May 2016
SRR1544762_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544762_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences532649
Sequences flagged as poor quality0
Sequence length52
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTGCT34330.6445144926583923Illumina Single End Adapter 2 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTGCTT28090.5273641741559639Illumina Single End Adapter 2 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTGCT21820.40965063296842763Illumina Single End Adapter 2 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9840.18473704071536792No Hit
GCCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTGC9580.17985577744443337No Hit
CGCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTGC9530.17891707296925366No Hit
TCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTGCT9330.17516225506853483Illumina Single End Adapter 2 (95% over 21bp)
ACCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTGC8870.16652617389688146No Hit
CGTTCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCT6580.12353350893365049No Hit
ACTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTGCT6360.11940320924285974Illumina Single End Adapter 2 (95% over 21bp)
AGCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTGC5970.11208131433645797No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCG5840.1096406827009907No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGAGG351.0181793E-746.0000041
TACGCGG750.046.0000041
TACGAGA351.0181793E-746.00000434
GCGATGA351.0181793E-746.0000048
TGATCGG206.3083903E-446.01
CGGGTTG253.4146702E-546.05
GTTCTCG253.4146702E-546.032
CACGGGA950.046.03
CGACGGC206.3083903E-446.02
GACACGA850.046.024
TAGCGCT206.3083903E-446.02
TTCACGG1050.046.01
ATAGCGG1000.046.01
CGACCAA800.046.028
ACTTATG206.3083903E-446.031
GGATACT206.3083903E-446.08
GCGTTGT253.4146702E-546.046
TAACCTA206.3083903E-446.031
GAGCGTT253.4146702E-546.044
TGCGATG253.4146702E-546.015
Produced by FastQC (version 0.11.5)