FastQCFastQC Report
Thu 26 May 2016
SRR1544761_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544761_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences375815
Sequences flagged as poor quality0
Sequence length52
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTGCT37540.99889573327302Illumina Single End Adapter 2 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTGCTT29590.7873554807551588Illumina Single End Adapter 2 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTGCT21370.5686308423027286Illumina Single End Adapter 2 (95% over 21bp)
TGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTGCTTG15310.4073812913268497Illumina Single End Adapter 2 (95% over 23bp)
ACCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTGC8400.22351422907547597No Hit
GCCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTGC8140.21659593150885412No Hit
CGCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTGC8080.21499940130117212No Hit
TCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTGCT7560.20116280616792837Illumina Single End Adapter 2 (95% over 21bp)
AGCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTGC6280.16710349507071298No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA5830.1551295185130982No Hit
ACTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTGCT5750.15300081156952222Illumina Single End Adapter 2 (95% over 21bp)
CGTTCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCT4900.13038330029402764No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4360.11601452842488989No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCG4230.11255537964157897No Hit
GGCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTGC4180.11122493780184399No Hit
AGGCCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCT4180.11122493780184399No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGACGG351.0171061E-746.0000041
TCACGAC405.5933924E-946.024
CGGGTCT405.5933924E-946.05
ATGGGCC253.412737E-546.04
AGCAACG206.306006E-446.09
CTGTCGG206.306006E-446.01
CAAACGG301.8580358E-646.01
ACCGGGT206.306006E-446.03
CACGGGT405.5933924E-946.03
CGACGGT405.5933924E-946.027
GACACGA453.092282E-1046.024
GCGCGAG206.306006E-446.016
GGCACCG850.046.07
CCGTGGG253.412737E-546.02
CGCGAGT206.306006E-446.017
ACTTAAC206.306006E-446.034
ACGGGTG206.306006E-446.04
ACGGGTC405.5933924E-946.04
ACGGGTA206.306006E-446.04
GTAATCC405.5933924E-946.08
Produced by FastQC (version 0.11.5)