##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1544761_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	375815
Sequences flagged as poor quality	0
Sequence length	52
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.881968521746074	34.0	33.0	34.0	31.0	34.0
2	33.21272966752258	34.0	33.0	34.0	31.0	34.0
3	33.29795245000865	34.0	34.0	34.0	31.0	34.0
4	36.56644625680188	37.0	37.0	37.0	35.0	37.0
5	36.55848223194923	37.0	37.0	37.0	35.0	37.0
6	36.51162939212112	37.0	37.0	37.0	35.0	37.0
7	36.34828306480582	37.0	37.0	37.0	35.0	37.0
8	36.41028431542115	37.0	37.0	37.0	35.0	37.0
9	38.25010443968442	39.0	39.0	39.0	37.0	39.0
10	38.113382914465895	39.0	39.0	39.0	35.0	39.0
11	37.41538256855102	39.0	35.0	39.0	35.0	39.0
12	37.3344305043705	39.0	35.0	39.0	35.0	39.0
13	37.30624642443755	39.0	35.0	39.0	35.0	39.0
14	38.56735627902026	41.0	35.0	41.0	35.0	41.0
15	38.65965435121004	41.0	35.0	41.0	35.0	41.0
16	38.68247408964517	41.0	35.0	41.0	35.0	41.0
17	38.7601452842489	41.0	36.0	41.0	35.0	41.0
18	38.738919415137765	41.0	36.0	41.0	35.0	41.0
19	38.64826842994559	41.0	35.0	41.0	35.0	41.0
20	38.567635671806606	40.0	35.0	41.0	35.0	41.0
21	38.51641366097681	40.0	35.0	41.0	35.0	41.0
22	38.4977901361042	40.0	35.0	41.0	35.0	41.0
23	38.47014089379083	40.0	35.0	41.0	35.0	41.0
24	38.39365645330815	40.0	35.0	41.0	35.0	41.0
25	38.37454332583851	40.0	35.0	41.0	35.0	41.0
26	38.329526495749235	40.0	35.0	41.0	35.0	41.0
27	38.32061785719037	40.0	35.0	41.0	35.0	41.0
28	38.38087090722829	40.0	36.0	41.0	35.0	41.0
29	38.38457485731011	40.0	36.0	41.0	35.0	41.0
30	38.32650107100568	40.0	35.0	41.0	35.0	41.0
31	38.29169405159453	40.0	35.0	41.0	35.0	41.0
32	38.24101752191903	40.0	35.0	41.0	35.0	41.0
33	38.16394502614318	40.0	35.0	41.0	35.0	41.0
34	38.06314276971382	40.0	35.0	41.0	35.0	41.0
35	38.01169724465495	40.0	35.0	41.0	35.0	41.0
36	37.995396671234516	40.0	35.0	41.0	35.0	41.0
37	37.886675624975055	40.0	35.0	41.0	35.0	41.0
38	37.89323470324495	40.0	35.0	41.0	35.0	41.0
39	37.83624921836542	40.0	35.0	41.0	35.0	41.0
40	37.801897210063466	40.0	35.0	41.0	35.0	41.0
41	37.76720194776685	40.0	35.0	41.0	35.0	41.0
42	37.712491518433275	40.0	35.0	41.0	35.0	41.0
43	37.65556989476205	40.0	35.0	41.0	35.0	41.0
44	37.54595745246996	40.0	35.0	41.0	35.0	41.0
45	37.564682090922396	40.0	35.0	41.0	35.0	41.0
46	37.548602370847355	40.0	35.0	41.0	35.0	41.0
47	37.47804637920253	39.0	35.0	41.0	35.0	41.0
48	37.44761917432779	39.0	35.0	41.0	35.0	41.0
49	37.39658076447188	39.0	35.0	41.0	35.0	41.0
50	37.33684658675146	39.0	35.0	41.0	35.0	41.0
51	37.279299655415564	39.0	35.0	41.0	34.0	41.0
52	36.87538283463938	38.0	35.0	40.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	0.0
14	3.0
15	5.0
16	2.0
17	17.0
18	21.0
19	54.0
20	98.0
21	150.0
22	234.0
23	396.0
24	670.0
25	1121.0
26	1588.0
27	1800.0
28	1778.0
29	1823.0
30	2155.0
31	2840.0
32	3661.0
33	5649.0
34	17308.0
35	80129.0
36	11555.0
37	17566.0
38	34766.0
39	189316.0
40	1109.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.512579327594693	21.845855008448307	23.324508069129756	23.317057594827244
2	26.579034897489457	24.087915596769687	26.265848888415842	23.067200617325014
3	26.27303327435041	24.5075369530221	26.66870667748759	22.550723095139897
4	25.812434309434163	26.353391961470408	24.941793169511595	22.892380559583838
5	23.917352952915664	33.652994159360325	21.38498995516411	21.044662932559905
6	82.36792038636031	2.6678019770365737	10.452749358061812	4.511528278541303
7	83.37080744515254	2.596756382794726	7.621303034737837	6.411133137314902
8	71.60198501922488	4.534145789816798	15.002594361587482	8.861274829370833
9	47.90149408618603	18.200710455942417	16.758511501669705	17.139283956201854
10	40.9653685989117	19.55563242552852	27.60028205367002	11.87871692188976
11	38.78371007011429	17.32448146029296	24.726527679842476	19.165280789750273
12	19.280763141439273	34.15297420273273	29.17579128028418	17.390471375543818
13	12.653034072615515	37.497438899458515	30.144086851243294	19.705440176682675
14	11.406410068783842	24.71029628939771	44.92343307212325	18.959860569695195
15	11.937788539573994	18.351582560568364	48.81470936498011	20.89591953487753
16	12.947061719196945	22.260154597341778	28.761491691390713	36.03129199207057
17	17.211127815547545	22.8117557840959	33.67507949389992	26.30203690645663
18	22.124183441320863	28.635897981719726	28.8689913920413	20.370927184918113
19	26.37228423559464	21.397762196825564	26.05244601732235	26.177507550257438
20	17.600947274589892	31.534664662134293	28.327235474901215	22.5371525883746
21	18.206298311669304	24.42850870774184	27.01675026276226	30.34844271782659
22	16.13400210209811	30.860928914492504	23.60523129731384	29.39983768609555
23	17.057860915610075	22.24658409057648	38.57828985005921	22.117265143754242
24	18.502720753562258	22.449077338584146	33.60988784375291	25.438314064100688
25	14.630336734829639	31.850777643255324	29.423519550842837	24.095366071072203
26	15.864986762103694	28.549419262136954	28.569109801365034	27.016484174394318
27	19.005627768982077	24.895759881856762	35.98233173236832	20.116280616792835
28	14.384471082846614	23.83539773558799	31.10173888748453	30.678392294080865
29	17.25928981014595	25.58492875483948	30.289637188510305	26.866144246504263
30	23.48549153173769	25.219855514016203	21.709617764059445	29.58503519018666
31	24.57911472399984	24.25608344531219	25.286111517635007	25.878690313052964
32	22.914998070859333	24.227878078309807	24.65894123438394	28.19818261644692
33	17.04854782273193	26.151430890198636	27.022870295225044	29.777150991844394
34	17.786676955416894	19.613639689740964	31.565264824448196	31.034418530393943
35	27.351223341271634	21.20697683700757	24.647499434562217	26.794300387158575
36	22.334659340366937	26.038609422189108	27.339249364714018	24.287481872729934
37	20.32595825073507	26.51943110306933	28.901188084562886	24.253422561632718
38	21.852773306014928	24.810611604113724	26.33290315713849	27.00371193273286
39	29.65049292870162	20.44356930936764	27.08167582454133	22.824261937389405
40	18.412782885196176	19.08864733978154	32.67458723041922	29.823982544603062
41	23.00733073453694	22.78674347750888	24.744355600494924	29.46157018745926
42	22.83197850005987	19.33690778707609	27.800114417998216	30.030999294865822
43	23.88409190692229	25.02907015419821	25.782898500592044	25.303939438287454
44	16.509718877639266	27.98504583372138	27.142343972433245	28.362891316206113
45	20.78017109482059	25.348376195734602	26.60963505980336	27.261817649641447
46	24.548780650053885	20.02847145537033	28.829078136849244	26.59366975772654
47	22.19070553330761	17.45060734669984	33.194523901387655	27.164163218604898
48	20.422814416667777	16.66138924736905	28.8067267139417	34.10906962202147
49	20.20967763394223	21.370089006559077	32.81800886074265	25.602224498756037
50	19.804957226294853	22.757739845402657	29.483123345262964	27.95417958303953
51	20.62717028325107	18.51948432074292	30.89631866742945	29.957026728576558
52	21.977834838950017	16.464749943456223	36.20637813817969	25.351037079414073
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	119.0
1	127.5
2	136.0
3	506.0
4	876.0
5	674.5
6	473.0
7	504.0
8	535.0
9	594.5
10	654.0
11	646.0
12	638.0
13	594.0
14	523.0
15	496.0
16	471.0
17	446.0
18	452.5
19	459.0
20	583.0
21	707.0
22	697.5
23	688.0
24	709.0
25	730.0
26	1056.0
27	1382.0
28	1651.5
29	1921.0
30	2143.5
31	2366.0
32	2701.5
33	3037.0
34	3575.5
35	4114.0
36	4683.0
37	5252.0
38	5841.0
39	7710.0
40	8990.0
41	12992.5
42	16995.0
43	22326.5
44	27658.0
45	30837.0
46	34016.0
47	35921.0
48	37826.0
49	44378.5
50	50931.0
51	52616.0
52	54301.0
53	46302.0
54	38303.0
55	31207.5
56	24112.0
57	19191.0
58	14270.0
59	12375.0
60	10480.0
61	9706.5
62	8933.0
63	8045.0
64	5449.5
65	3742.0
66	2912.5
67	2083.0
68	1728.0
69	1373.0
70	1104.0
71	835.0
72	762.5
73	690.0
74	560.5
75	431.0
76	404.0
77	377.0
78	275.5
79	174.0
80	108.5
81	43.0
82	42.0
83	41.0
84	32.5
85	24.0
86	18.5
87	13.0
88	7.5
89	1.5
90	1.0
91	2.0
92	3.0
93	2.5
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	375815.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.128291718856936
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.7287382673647	22.40265244241716
2	12.178824849911019	7.825696751416104
3	4.619324895854642	4.45233053394589
4	2.225332383481971	2.8598451195171184
5	1.3163551293413254	2.1146120800545876
6	0.9191271140970472	1.771799042905262
7	0.6469573317366566	1.4549943718582021
8	0.5789267735484792	1.4879942611537733
9	0.4959825463748103	1.4341564743652253
>10	7.020046145647332	41.665596106666754
>50	0.18661511225292923	4.0991420341941565
>100	0.0746460449011717	4.331505802887994
>500	0.005805803492313354	1.333426947871518
>1k	0.003317601995607631	2.7662480307462487
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTGCT	3754	0.99889573327302	Illumina Single End Adapter 2 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTGCTT	2959	0.7873554807551588	Illumina Single End Adapter 2 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTGCT	2137	0.5686308423027286	Illumina Single End Adapter 2 (95% over 21bp)
TGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTGCTTG	1531	0.4073812913268497	Illumina Single End Adapter 2 (95% over 23bp)
ACCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTGC	840	0.22351422907547597	No Hit
GCCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTGC	814	0.21659593150885412	No Hit
CGCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTGC	808	0.21499940130117212	No Hit
TCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTGCT	756	0.20116280616792837	Illumina Single End Adapter 2 (95% over 21bp)
AGCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTGC	628	0.16710349507071298	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA	583	0.1551295185130982	No Hit
ACTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTGCT	575	0.15300081156952222	Illumina Single End Adapter 2 (95% over 21bp)
CGTTCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCT	490	0.13038330029402764	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	436	0.11601452842488989	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCG	423	0.11255537964157897	No Hit
GGCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTGC	418	0.11122493780184399	No Hit
AGGCCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCT	418	0.11122493780184399	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.7995955456807206	0.0
2	0.0	0.0	0.0	2.7518859013078245	0.0
3	0.0	0.0	0.0	4.234264199140535	0.0
4	0.0	0.0	0.0	5.166105663690912	0.0
5	0.0	0.0	0.0	9.614304910660831	0.0
6	0.0	0.0	0.0	10.336202652901028	0.0
7	0.0	0.0	0.0	13.251998988864202	0.0
8	0.0	0.0	0.0	16.430690632359006	0.0
9	0.0	0.0	0.0	19.147186780729882	0.0
10	0.0	0.0	0.0	20.770059736838604	0.0
11	0.0	0.0	0.0	22.264944187964822	0.0
12	0.0	0.0	0.0	23.69862831446323	0.0
13	0.0	0.0	0.0	24.269121775341592	0.0
14	0.0	0.0	0.0	24.696193605896518	0.0
15	0.0	0.0	0.0	26.11923419767705	0.0
16	0.0	0.0	0.0	27.58804198874446	0.0
17	0.0	0.0	0.0	29.669917379561753	0.0
18	0.0	0.0	0.0	30.487074757526976	0.0
19	0.0	0.0	0.0	31.452443356438675	0.0
20	0.0	0.0	0.0	32.64824448199247	0.0
21	0.0	0.0	0.0	33.603767811290126	0.0
22	0.0	0.0	0.0	34.76817050942618	0.0
23	0.0	0.0	0.0	35.48314995409976	0.0
24	0.0	0.0	0.0	36.114045474502085	0.0
25	0.0	0.0	0.0	36.78299163152083	0.0
26	0.0	0.0	0.0	37.36386253874912	0.0
27	0.0	0.0	0.0	37.94499953434536	0.0
28	0.0	0.0	0.0	38.496600721099476	0.0
29	0.0	0.0	0.0	39.267192634673975	0.0
30	0.0	0.0	0.0	40.01197397655761	0.0
31	0.0	0.0	0.0	40.615196306693456	0.0
32	0.0	0.0	0.0	41.16440269813605	0.0
33	0.0	0.0	0.0	41.69445072708646	0.0
34	0.0	0.0	0.0	42.47994358926599	0.0
35	0.0	0.0	0.0	43.03473783643548	0.0
36	0.0	0.0	0.0	43.61161741814457	0.0
37	0.0	0.0	0.0	44.12676449848995	0.0
38	0.0	0.0	0.0	44.62461583491878	0.0
39	0.0	0.0	0.0	45.173023961257535	0.0
40	0.0	0.0	0.0	45.74165480356026	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATGACGG	35	1.0171061E-7	46.000004	1
TCACGAC	40	5.5933924E-9	46.0	24
CGGGTCT	40	5.5933924E-9	46.0	5
ATGGGCC	25	3.412737E-5	46.0	4
AGCAACG	20	6.306006E-4	46.0	9
CTGTCGG	20	6.306006E-4	46.0	1
CAAACGG	30	1.8580358E-6	46.0	1
ACCGGGT	20	6.306006E-4	46.0	3
CACGGGT	40	5.5933924E-9	46.0	3
CGACGGT	40	5.5933924E-9	46.0	27
GACACGA	45	3.092282E-10	46.0	24
GCGCGAG	20	6.306006E-4	46.0	16
GGCACCG	85	0.0	46.0	7
CCGTGGG	25	3.412737E-5	46.0	2
CGCGAGT	20	6.306006E-4	46.0	17
ACTTAAC	20	6.306006E-4	46.0	34
ACGGGTG	20	6.306006E-4	46.0	4
ACGGGTC	40	5.5933924E-9	46.0	4
ACGGGTA	20	6.306006E-4	46.0	4
GTAATCC	40	5.5933924E-9	46.0	8
>>END_MODULE