FastQCFastQC Report
Thu 26 May 2016
SRR1544760_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1544760_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences657178
Sequences flagged as poor quality0
Sequence length52
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCTGCT34220.5207112837009151Illumina Single End Adapter 1 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCTGCTT25820.3928920322956642Illumina Single End Adapter 1 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCTGCT18380.2796806953367276Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8930.13588403750582034No Hit
CGCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCTGC8650.13162339579231197No Hit
TCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCTGCT8220.12508026744656697Illumina Single End Adapter 1 (95% over 21bp)
ACCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCTGC7840.1192979679782342No Hit
GCCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCTGC6830.1039292246545076No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGGA1050.046.0000043
TACGAGA351.01867045E-746.00000434
ATCGGGT351.01867045E-746.0000043
CGGTAGA351.01867045E-746.00000424
TCAACGG700.046.0000041
TCACGAC600.046.024
ACAACGT206.309472E-446.037
CGGGTGC501.6370905E-1146.05
AACCGAT206.309472E-446.027
GGGTGCG253.4155484E-546.06
CTCACGG206.309472E-446.01
CAACGTA206.309472E-446.038
CGACGGT600.046.027
CAACGAC453.092282E-1046.011
ACGCATG206.309472E-446.019
CCGATGT206.309472E-446.029
CGACGCG453.092282E-1046.014
CTATCGG301.8601786E-646.01
GAGTCGT206.309472E-446.031
CCAGGTA206.309472E-446.042
Produced by FastQC (version 0.11.5)